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Wnt3a通过整合素连接激酶调节血管平滑肌细胞迁移和黏附 预览 被引量:1

Wnt3a regulates migration and adhesion of vascular smooth muscular cells via integrin-linked kinase
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摘要 目的探讨重组Wnt3a蛋白对大鼠血管平滑肌细胞(VSMCs)迁移和黏附的影响及相关机制。方法原代培养大鼠VSMCs,实验分为Wnt3a组和对照组,通过Transwell实验检测VSMCs的迁移能力,细胞基质黏附实验检测VSMCs黏附细胞外基质的能力,Western blot检测VSMCs中β-连环蛋白(β-catenin)、磷酸化β-catenin(Ser675)、糖原合成激酶3β(GSK-3β),磷酸化GSK-3β(Ser9)、整合素连接激酶(ILK)的蛋白表达水平。结果Wnt3a组中VSMCs迁移的数量明显高于对照组(P〈0.05)。与对照组相比,Wnt3a组中VSMCs黏附于胶原Ⅰ的数量和光密度值均显著增加(P〈0.05),且Wnt3a组中磷酸化β-catenin(Ser675)、磷酸化GSK-3β(Ser9)和ILK蛋白的表达水平均显著上调(P〈0.05)。结论 Wnt3a可以通过ILK调节VSMCs迁移和黏附。 Objective To investigate the effects and related mechanism of recombinant Wnt3 aprotein on vascular smooth muscle cells(VSMCs)migration and adhesion.Methods Primary rat VSMCs were cultured.The experiment was divided into the Wnt3 agroup and control group.The VSMC migration ability was detected by Transwell experiment.And the ability of VSMC adhesion to extracelluar matrix was detected by the celluar matrix adhesion assay.The protein expression levels ofβ-catenin,phosphor-β-catenin(Ser675),GSK-3β,phosphor-GSK-3β(Ser9)and integrin-linked kinase(ILK)in VSMCs were detected by Western blot and analyzed.Results The number of VSMCs migration in the Wnt3 agroup was significantly higher than that in the control group(P〈0.05).Compared with the control group,the number of VSMCs adhesion to collagenⅠand the optical density value in the Wnt3 agroup were significantly increased(P〈0.05),and the protein expression levels ofβ-catenin,Ser675,GSK-3β,Ser9 and ILK in the Wnt3 agroup were significantly up-regulated(P〈0.05).Conclusion Wnt3 acan regulate VSMCs migration and adhesion by ILK.
作者 吴校林 朱锐 李彬 刘文卫 周青 赵玉勤 Wu Xiaolin , Zhu RuiA , Li Bin , Liu Wenwei , Zhou Qing , Zhao Yuqin (Department of Cardiology,Xiangyang Municipal Central Hospital Affiliated to Hubei University of Arts and Science , Xiangyang , Hubii 441000 , China)
出处 《重庆医学》 北大核心 2017年第36期5049-5051,共3页 Chongqing Medical Journal
基金 湖北省自然科学基金青年项目(2016CFB344)
关键词 WNT蛋白质类 平滑 血管 细胞运动 细胞黏附 蛋白质丝氨酸苏氨酸激酶 Wnt proteins muscle, smooth, vascular cell movement cell adhesion protein-serine-threonine kinases
作者简介 吴校林(1982-),博士,主治医师,主要从事冠心病相关机制的研究.;通信作者,E-mail:zhurui@medmail.com.cn
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