期刊文献+

海马Wnt/β-catenin信号通路在氯化锂预处理改善老龄大鼠术后认知功能中的作用

Role of Wnt/β-catenin signaling pathway in lithium chloride preconditioning-induced improvement in postoperative cognitive function of aged rats
分享 导出
摘要 目的评价海马Wnt/β-catenin信号通路在氯化锂预处理改善老龄大鼠术后认知功能中的作用。方法清洁级健康雄性老龄SD大鼠100只,18月龄,体重540~650 g,采用随机数字表法分为5组(n=20):对照组(C组)、手术组(S组)、氯化锂预处理组(L组)、sFRP-1组(F组)及氯化锂预处理+sFRP-1组(L+F组)。L组和L+F组术前连续5 d腹腔注射氯化锂2 mmol/kg,1次/d;F组和L+F组术前1 d脑室注射sFRP-1(浓度10 μg/ml)蛋白液10 μl。于术后1 d每组随机处死10只大鼠,取海马组织,采用Western blot法检测海马组织磷酸化糖原合成酶激酶-3β(p-GSK-3β)、β-catenin及Wnt的表达。术后3~7 d行Morris水迷宫,术后第7天采用ELISA法检测脑脊液β-淀粉样蛋白42(Aβ-42)和磷酸化tau-181蛋白(p-tau-181)浓度。结果与C组比较,其余4组术后3~7 d逃避潜伏期延长,脑脊液Aβ-42浓度升高,S组海马Wnt、p-GSK-3β和β-catenin表达下调,L组和L+F组海马Wnt、p-GSK-3β和β-catenin表达上调,S组和L+F组脑脊液p-tau-181浓度升高(P〈0.05)。与S组比较,L组术后3~7 d和L+F组术后5~7 d逃避潜伏期缩短,L组和L+F组海马Wnt、p-GSK-3β和β-catenin表达上调,脑脊液Aβ-42和p-tau-181浓度降低(P〈0.05)。与L组比较,L+F组术后3~6 d逃避潜伏期延长,海马Wnt、p-GSK-3β和β-catenin表达下调,脑脊液p-tau-181浓度升高(P〈0.05)。与F组比较,L+F组术后3~7 d逃避潜伏期缩短,海马Wnt、p-GSK-3β和β-catenin表达上调,脑脊液Aβ-42和p-tau-181浓度降低(P〈0.05)。结论氯化锂预处理改善老龄大鼠术后认知功能的部分机制与激活Wnt/β-catenin信号通路有关。 Objective To evaluate the role of Wnt/β-catenin signaling pathway in lithium chloride preconditioning-induced improvement in postoperative cognitive function of aged rats.Methods A total of 100 pathogen-free healthy male Sprague-Dawley rats, aged 18 months, weighing 540-650 g, were divided into 5 groups(n=20 each)using a random number table: control group(group C), surgery group(group S), lithium chloride preconditioning group(group L), secreted frizzled-related protein 1(sFRP-1) group(group F)and lithium chloride preconditioning plus sFRP-1 group(group L+ F). Lithium chloride 2 mmol/kg was intraperitoneally injected once a day for 5 consecutive days before operation in L and L+ F groups.In F and L + F groups, sFRP-1 10 μl(concentration 10 μg/ml)was injected into the ventricle at 1 day before operation.Ten rats in each group were randomly sacrificed at 1 day after operation, and the hippocampi were removed for determination of the expression of phosphorylated glycogen synthase kinase-3 beta(p-GSK-3β), β-catenin and Wnt in hippocampal tissues(by Western blot). The rest rats underwent Morris water maze test at day 3-7 after operation, and the concentrations of amyloid beta 42(Aβ-42)and phosphorylated tau-181 protein(p-tau-181)in cerebrospinal fluid(CSF)were detected by enzyme-linked immunosorbent assay at day 7 after operation.Results Compared with group C, the escape latency was significantly prolonged at 3-7 days after operation, and the concentration of Aβ-42 in CSF was increased in the other four groups, and the expression of Wnt, p-GSK-3β and β-catenin in hippocampal tissues was down-regulated in group S, the expression of Wnt, p-GSK-3β and β-catenin in hippocampal tissues was significantly up-regulated in L and L+ F groups, and the concentration of p-tau-181 in CSF was significantly increased in S and L+ F groups(P〈0.05). Compared with group S, the escape latency was significantly shortened at 3-7 days after operation in group L
作者 陈英 周志东 张静 张列亮 魏根 徐国海 华福洲 Chen Ying, Zhou Zhidong, Zhaag Jing, Zhang Lieliang, Wei Gen, Xu Guohai, Hua Fuzou(Department of Anesthesiology, Second Affiliated Hospital, Nanchang University Key Laboratory of Anesthesiology in Jiangxi Province, Nanchang 330006, China)
出处 《中华麻醉学杂志》 CSCD 北大核心 2017年第12期1493-1497,共5页 Chinese Journal of Anesthesilolgy
基金 国家自然科学基金(81560193) 南昌大学第二附属医院青年科学基金(2016YNQN12019)
关键词 氯化锂 WNT蛋白质类 老年人 认知障碍 预处理 Lithium chloride Wnt proteins Aged Cognition disorders Preconditioning
作者简介 通信作者:华福洲,Email:huafuzhou@126.com
  • 相关文献

参考文献4

二级参考文献52

  • 1李大江,刘焱斌,刘凯.生物信息学在未来感染病学教学中的地位和作用[J].华西医学,2006,21(3):474-476. 被引量:6
  • 2杜怡峰,王蓉,姬志娟,盛树力.APP17肽对APP转基因小鼠学习记忆能力和海马神经细胞凋亡的影响[J].中国神经免疫学和神经病学杂志,2007,14(1):7-10. 被引量:7
  • 3Kawano Y,Kypta R. Secreted antagonists of the Wnt signaling pathway. J Cell Sei, 2003,116 (13) : 2627-2634. 被引量:1
  • 4Ko J, Ryu KS, Lee YDI, etal. Human secreted frizzled-related protein is downregulated and induces apoptosis in human cervical cancer. Exp Cell Res,2002,280(2) :280 -287. 被引量:1
  • 5Cook D, Fry MJ, Hughes K, et al. Wingless inactivates glycogen synthase kinase-3 via an intracellular signaling pathway which involves a protein kinase C. EMBO J, 1996, 15 (17): 4526 -4536. 被引量:1
  • 6Behrens J, Von Kries JP, Kuhl M, et al. Functional interaction of β-catenin with the transcriptional factor LEF-1. Nature, 1996,382 (6592) : 638-642. 被引量:1
  • 7Hay E, Faucheu C, Suc-Royer I, et al. Interaction between LRP5 and Fratl mediates the activation of the Wnt canonical pathway. J Biol Chem,2005,280 (14) :13616-13623. 被引量:1
  • 8Kleber M, Sommer L. Wnt signaling and the regulation of stem cell function. Curr ()pin Cell Biol,2004,16(6) :681 687. 被引量:1
  • 9De Almeida VI, Miao I., Ernst JA, et al. The soluble wnt receptor FrizzledSCRD-hFc inhibits the growth of teratocarcinomas in vivo. Cancer Res,2007.67(11 ) :5371-5379. 被引量:1
  • 10Yoshino K,Rubin JS, Higinbotham KG,et al. Secreted Frizzled related proteins can regulate metanephric development. Mech Dev,2001,102(1 2) :45-55. 被引量:1

共引文献6

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈