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缺氧微环境中HIF-1α基因沉默的人绒毛膜癌细胞系JEG-3侵袭和迁移能力观察 预览 被引量:1

Effects of silencing HIF-1α on invasion and migration of human choriocarcinoma JEG-3 cells under hypoxia
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摘要 目的观察缺氧微环境中缺氧诱导因子1α(HIF-1α)沉默的人绒毛膜癌细胞系JEG-3的侵袭、迁移能力。方法取对数生长期JEG-3细胞分为A、B、C、D组,其中A、B、C组置于体外缺氧微环境培养,D组不处理。当细胞融合到60%~70%时A、C组分别用HIF-1αsiRNA和无关序列转染,B、D组不做处理。采用Transwell细胞侵袭试验及划痕试验检测各组细胞侵袭能力和迁移能力。采用实时荧光定量PCR法和蛋白印迹法检测各组细胞HIF-1α、CXCR4的mRNA和蛋白表达。结果 A组细胞的侵袭和迁移能力明显低于其余3组(P均〈0.05),B、C组细胞侵袭和迁移能力显著高于D组(P均〈0.05)。A组细胞中HIF-1α、CXCR4的mRNA和蛋白表达均低于其余3组(P均〈0.05),B、C组细胞HIF-1α蛋白、CXCR4 mRNA和蛋白表达均高于D组(P均〈0.05)。结果缺氧微环境中HIF-1α沉默的JEG-3细胞侵袭、迁移能力下降,其机制可能与HIF-1α下调细胞CXCR4表达有关。 Objective To investigate the effects of hypoxia inducible factor 1α( HIF-1α) on the invasion and migration of human choriocarcinoma JEG-3 cells under hypoxia. Methods JEG-3 cells in the logarithmic phase were divided into groups A,B,C,and D; and cells in the groups A,B,and C were cultured under hypoxia in vitro,but the group D was not treated. When JEG-3 cells were fused to 60% to 70%,cells in the groups A and C were transfected with HIF-1αsiRNA and NC siRNA,respectively,and we did not do the treatment on the cells in the groups B and D. Real-time PCR and Western blotting were used to detect the mRNA and protein expression levels of HIF-1α and CXCR4 in each group,respectively. The invasion and migration abilities of JEG-3 cells were separately analyzed by Transwell invasion assay and Scratch test. Results The invasion and migration abilities of JEG-3 cells in the group A were significantly lower than those in the other 3 groups( all P〈0. 05),the invasion and migration abilities of JEG-3 cells in the groups B and C were higher than those in the group D( all P〈0. 05). The mRNA and protein expression levels of HIF-1α and CXCR4 in the group A were significantly lower than those in the other 3 groups( all P〈0. 05),while the mRNA and protein expression levels of HIF-1α and CXCR4 in the groups B and C were significantly higher than those in the group D( all P〈0. 05).Conclusion Silencing HIF-1α can inhibit the invasion and migration abilities of of JEG-3 cells under hypoxia,and its molecular mechanism may be related to the down-regulation of CXCR4.
作者 张展 李鹏云 闫欢 王艳 余洁 ZHANG Zhan, LI Pengyun, YAN Huan, WANG Yan, YU Jie ( The Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China)
出处 《山东医药》 2018年第3期15-18,共4页 Shandong Medical Journal
基金 郑州市科技创新项目(131PCXTD624)
关键词 缺氧诱导因子1Α 趋化因子受体4 滋养细胞 细胞迁移 细胞侵袭 子痫 缺氧微环境 hypoxia-inducible factor 1α chemokine receptor 4 trophoblast cells cell migration cell invasion eclampsia hypoxia microenvironment
作者简介 张展(1959-),女,博士生导师,教授,主要研究方向为子痫前期、生殖医学、多囊卵巢综合征等。E-mail:zhmagzhanmd@126.com
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