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动物源性食品中56种兽药残留物的UPLC-MS/MS高通量分析

UPLC-MS/MS high-throughput detection of 56 veterinary drug residues in food products of animal origin
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摘要 建立了超高效液相色谱串联质谱法同时测定56种动物源性兽药残留物的分析方法。以乙腈-0.5%乙酸水溶液(85:15,V/V)为提取溶剂,采用固相萃取柱(Oasis PRi ME HLB)进行样品净化,通过UPLC-MS/MS分析测定,基质曲线外标法定量。方法定量限为1.0μg/kg(喹诺酮类)、1.5μg/kg(糖皮质类)、10μg/kg(磺胺类)、1.0μg/kg(β-受体激动剂类)。56种兽药残留物在牛肉、猪肉、鸡肉和奶等基质样品中回收率范围为63.2%-101.5%,基质标准工作曲线线性相关系数大于0.937,精密度RSD〈15%(n=3)。方法适用于动物源性食品中兽药残留物的分析。 A method for simultaneous determination of 56 veterinary drug residues in food products of animal originwas established by UPLC-MS/MS.After extraction by the mixed solvent of acetonitrile and0.5% acetic acid in water(85: 15),the sample extracts were subjected to a cleanup procedure by using Oasis PRi ME HLB column.The residue contentswas analyzed by UPLC-MS/MS,and the quantitative information was obtained bymatrix-matched calibration with external standard.The LOQs of 4-quinolones,glucocorticoid,sulfonamides and β-agonist were determined to be 1.0,1.5,10 and1.0 μg/kg,respectively.56 veterinary drugs exhibited good linear relationship with correlation coefficient of 0.937 and RSD less than 15%(n = 3).The recoveries of veterinary drugs in matrix including beef,pork,chicken and milk ranged from 63% to 101%.The method was quick and simple,and would be suitable for the detection of veterinary drug residues in food products of animal origin.
作者 王嘉琦 马立利 曹英华 王建凤 刘喆 范筱京 贾丽 WANG Jia-qi , MA Li-li, CAO Ying-hua, WANG Jian-feng, LIU Zhe, FAN Xiao-jing , JIA Li (Beijing Center For Physical & Chemical Analysis, Beijing 100089)
出处 《分析试验室》 CSCD 北大核心 2018年第3期306-310,共5页 Chinese Journal of Analysis Laboratory
基金 北京市科学技术研究院2016年青年骨干计划项目(201616)资助
关键词 兽药 超高效液相色谱串联质谱 固相萃取柱Oasis PRiME HLB Veterinary drug UPLC-MS/MS Oasis PRIME HLB Animal origin
作者简介 通信作者,E-mail:28255291@qq.com
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