目的：探讨叉状头/翅膀状螺旋转录因子（Foxp3）过表达对人乳头状瘤病毒（HPV）感染阳性宫颈癌细胞增殖、凋亡及细胞免疫的影响.方法：利用脂质体介导转染方法,建立Foxp3过表达HPV感染阳性SiHa细胞株,作为Foxp3过表达阳性细胞,同时利用pcDNA3.1空载体转染SiHa细胞株,作为本研究阴性对照,同时设置空白对照组,采用实时荧光定量聚合酶链式反应（qRT-PCR）方法及Western blot法检测三组Foxp3 mRNA及蛋白表达水平,采用酶联免疫吸附试验（ELISA）检测SiHa细胞中白细胞介素8（IL-8）、白细胞介素10（IL-10）水平,采用四甲基偶氮唑蓝（MTT）实验检测SiHa细胞增殖能力,采用利用流式细胞仪（FCM）检测SiHa细胞周期及凋亡率情况.结果：过表达组Foxp3mRNA及蛋白表达水平明显高于阴性对照组及空白对照组,差异有统计学意义（P〈0.05）,阴性对照组及空白对照组Foxp3mRNA及蛋白表达水平比较,差异无统计学意义（P〉0.05）;与阴性对照组及空白对照组比较,各时间点过表达组IL-8水平明显降低,36h、48hIL-10水平明显升高;MTT结果显示,Foxp3过表达可明显促进SiHa细胞增殖,FCM检测显示,Foxp3过表达可明显抑制SiHa细胞凋亡,抑制G0/G1期阻滞.结论：Foxp3过表达可促进HPV感染阳性宫颈癌细胞增殖,抑制细胞凋亡.
Objective： To explore the influence of forkhead/winged helix transcription factor（FOXP3） overexpression on cell proliferation and apoptosis of human papilloma virus（HPV） infected positive cervical cancer cells. Methods： Foxp3 overexpression of HPV infected positive SiHa cell lines were developed by using liposome mediated transfection method,as Foxp3 overexpression positive cell,and transfected SiHa cell lines by using pcDNA3.1 empty carrier were as the negative control group in the study,and meanwhile,the blank control group were set.Foxp3 mRNA and protein expression levels were detected in the three groups by using real-time fluorescence quantitative polymerase chain reaction（qRT-PCR） and Western blot method.Interleukin 8（IL-8） and interleukin 10（IL-10） levels in SiHa cells were tested by using Enzyme linked immunosorbent assay（ELISA） method.SiHa cell proliferation ability were tested by using methylthiazolyldiphenyl-tetrazolium bromide（MTT） experiment,and SiHa cell cycle and apoptosis rate were detected by using flow cytometry（FCM）. Results： The expression levels of Foxp3,mRNA and protein in the overexpression group were significantly higher than those in the negative control group and the blank control group,with statistically significant differences（ P 〈0.05）.There were no significant differences in the expression levels between the negative control group and the blank control group（ P 〉0.05）.Compared with those in the negative control group and the blank control group,IL-8 level decreased significantly in different time points,and IL-10 level increased significantly at 36 h and 48 h in the overexpression group.MTT result showed that Foxp3 overexpression could significantly promote the proliferation of SiHa cells.FCM showed that Foxp3 overexpression could significantly inhibit the apoptosis of SiHa cells,and inhibit G 0/G 1 blocking. Conclusion： FOXP3 overexpression can promote the proliferation of HPV infected positive cervical cancer cells,and inhibit the
Journal of Modern Oncology
human papilloma virus hpv
forkhead/winged helix transcription factor