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TLR7、TLR9激动剂对人外周血B细胞作用的研究

Human B cell responses to TLR7 and TLR9 agonists
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摘要 人B淋巴细胞被TLR7、TLR9配体刺激后,可被激活进行克隆性增殖,促进其行使免疫功能。本研究使用TLR7、TLR9激动剂刺激健康人PBMC。使用流式细胞术,用荧光素标记的CD19筛选出B细胞后,CFSE法测定B细胞增殖情况,Annexin V测定B细胞凋亡情况,并且测定B细胞表面CD80、CD86、CD40、HLA-DR表达和B细胞IgG、IgM分泌情况。结果表明,TLR7、TLR9激动剂能显著刺激B细胞增殖、表面CD80、CD86、CD40和HLA-DR的表达上调以及刺激B细胞分泌IgM。不仅如此,TLR9激动剂还能降低B细胞早期凋亡并且刺激IgG分泌增加,而TLR7激动剂在上述方面作用却不显著。TLR9信号通路在刺激B细胞增殖、降低其早期凋亡、刺激其CD80上调以及IgG分泌方面显著强于TLR7。课题组需要进一步研究以探寻TLR9与TLR7在人B细胞内受体表达和信号转导通路的区别,为TLR通路缺陷相关疾病的发病机制和治疗研究提供理论依据。 Human B cells will be activated upon stimulation by TLR7 and TLR9 agonists.In this study,we used TLR7 and TLR9 agonists to stimulate B cells in healthy human peripheral blood.After B cells were gated by fluorescein labelled CD19.The proliferation of CFSE marked B cells,the apoptosis of Annexin V marked B cells,the expression of CD80,CD86,CD40 and HLA-DR,and secretion of IgM and IgG were all detected by FACS.The results showed that TLR7 and TLR9 agonists could significantly promote cell proliferation,and enhance the expression of CD80,CD86,CD40 and HLA-DR,and the secretion of IgM by B cells.Moreover,TLR9 agonist,but not TLR7 agonist,could also reduce the early apoptosis of B cells and increase the secretion of IgG.We need further studies to find out the difference of receptor expression and signal transduction pathways induced by TLR7 and TLR9 in human B cells,which will help us for better understanding of pathogenesis and treatment of specific primary immunodeficiency diseases.
作者 龚若兰 吴静 陈同辛 GONG Ruo-lan;WU Jing;CHEN Tong-xin(1. Department of Allergy and Immunology, Shang-hai Children 's Medical Center, Shanghai J iao Tong University School of Medicine, Shanghai 200127, China; 2. Institute of Pediatric Translational Medicine, Shanghai Children ~ s Medical Center, Shang- hai Jiao Tong University School of Medicine, Shanghai 200127, China)
出处 《现代免疫学》 CSCD 北大核心 2018年第3期228-236,共9页 Current Immunology
基金 国家自然科学基金(81571605 81701626)
关键词 TLR7 TLR9 B淋巴细胞 免疫功能 TLR7 TLR9 B cell immunological function
作者简介 龚若兰(1991-),女,硕士生,儿童免疫学专业;通信作者:陈同辛(Email:tongxinc@yahoo.com)
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