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人工打洞沉香的化学成分及生物活性研究 被引量:1

Chemical Constituents and Bioactivities of Aquilariae Lignum Resinatum by Artificial Holing Method
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摘要 目的:研究人工打洞沉香的化学成分及其生物活性。方法:采用ODS、硅胶柱层析、Sephadex LH-20等柱色谱技术对人工打洞沉香的化学成分进行分离纯化,并根据理化性质和波谱数据对分离的化合物进行鉴定;分别采用Ellman法和PNPG法测定单体化合物的体外乙酰胆碱酯酶和α-葡萄糖苷酶抑制活性,采用DPPH法测定单体化合物的抗氧化活性。结果:从人工打洞沉香中分离得到8个化合物,分别鉴定为:5α,6β,7α,8β-四羟基-5,6,7,8-四氢-2-[2-(4-甲氧基苯)乙基]色酮(1)、8α-氯-5α,6β,7β-三羟基-5,6,7,8-四氢-2-[2-(3-羟基-4-甲氧基苯)乙基]色酮(2)、6-羟基-2-[2-(4-羟基-3-甲氧基苯)乙烯基]色酮(3)、6,7-二羟基-2-(2-苯乙基)色酮(4)、2-(2-苯乙基)色酮(5)、epi—guaidiolA(6)、2-OXO-12-hydroxy—hinesol(7)、cyclodebneyol(8)。结论:其中,化合物6—8为首次从沉香属植物中分到,化合物1、2为首次从该植物中分离得到。化合物1、2和7对乙酰胆碱酯酶具有一定的抑制活性,化合物3具有一定的体外α-葡萄糖苷酶抑制活性,化合物2、3和7具有一定的抗氧化活性。 Objective: To study the chemical constituens and biological activities of artificial holing Aquilariae Lignum Resinatum. Methods:The compounds were isolated by ODS, silica gel and Sephadex LH-20 column chromatographies from Aquilariae Lignum Resinatum induced by artificial holing, then structurally identified by spectroscopic analysis. The compounds were tested for their AChE and α-glucosidase inhibitory activities by Ellman and PNPG methods respectively, the antioxidant activity were determined by DPPH method. Results:Eight compounds were isolated from Aquilariae Lignum Resinatum and identified as 5α, 6β, 7α, 8β-tetra- hydroxy-5,6,7,8-tetrahydro-2- [ 2- (4-methoxyphenyl) ethyl ] chromone ( 1 ), 8α-chloro-5α, 6β, 7β-trihydroxy-5,6,7,8-tetrahydro-2- [ 2- (3-hydroxy-4-methoxyphenyl) ethyl ] chromone ( 2 ), 6-hydroxy-2-[ 2-( 4-hydroxy-3-methoxyphenyl ) ethylene ] chromone ( 3 ), 6, 7-di- hydroxy-2- (2-phenylethyl) chromone (4), 2- ( 2-phenylethyl ) chromone ( 5 ), epi-guaidiol A ( 6), 2-oxo- 12-hydroxy-hinesol ( 7 ) and cyclo- debneyol (8). Conclusion : Compounds 6 - 8 are obtained from the Aquilaria genus for the first time, compounds 1 and 2 are isolated from Aquilaria sinensis for the first time: Compounds 1,2 and 7 exhibited moderate inhibitory activity against acetyl cholinesterase, compound 3 showed positive α-glucosidase inhibitory activity;compounds 2,3 and 7 showed antioxidant activity.
作者 向盼 曾艳波 梅文莉 董文化 李薇 周丽曼 戴好富 XIANG Pan1,2,3 , ZENG Yan-bo1,2, MEI Wen-li1,2, DONG Wen-hua1,2, LI Wei1,2 ,ZHOU Li-man1,2 , DAI Hao-fu1 ,2 ( 1. Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscienee and Biotechnology,Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; 2. Hainan Engineering Research Center of Agarwood, Haikou 571101, China; 3. College of Pharmacy and Chemistry, Dali University, Dali 671003, China)
出处 《中药材》 北大核心 2017年第10期2339-2343,共5页 Jorunal of Chinese Medicinal Materials
基金 海南省重大科技计划项目(ZDKJ2016004-03) 海南省科协青年科技人才学术创新计划项目(HAST201628) 海南省自然科学基金(20168367)
关键词 沉香 化学成分 2-(2-苯乙基)色酮 倍半萜 生物活性 Aquilariae Lignum Resinatum Chemical eonstituents 2-(2-Phenylethyl)chromone Sesquiterpenoid Bioactivity
作者简介 向盼(1992-),女,在读硕士研究生,专业方向:天然产物化学;Tel:0898-66968571,E-mail:xiangpan0924@sina.com。;通讯作者:戴好富,Tel:0898-66961869,E-mail:daihaofu@itbb.org.cn。
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