期刊文献+

UFLC法同时测定甘草及其炮制品中2种活性成分的含量 预览 被引量:2

Simultaneous determination of two active components in Glycyrrhiza uralensis Fisch. and its processed products by UFLC
在线阅读 下载PDF
收藏 分享 导出
摘要 目的建立同时测定甘草及其炮制品中甘草苷和甘草酸含量的超快速液相色谱法,为甘草药材及其炮制品的质量控制提供参考。方法采用Shim-Pack XR-ODS柱(75 mm×3.0 mm,2.2μm);流动相为0.05%磷酸水(A)-乙腈(B),梯度洗脱(0-4 min,5%-20%B;4-12 min,20%-40%B;12-15 min,40%-45%B),平衡时间为2 min;流速为0.8 mL/min;检测波长为237 nm;柱温为30℃。结果甘草苷和甘草酸分别在10-500 mg/L(R=0.999 8)、10-500 mg/L(R=0.999 9)浓度范围内与峰面积呈良好的线性关系;平均回收率分别为98.9%和99.3%。结论该方法能够准确地测定甘草中甘草苷与甘草酸的含量,操作简便,具有良好的重现性和稳定性,可用于甘草药材的质量控制。 Objective To establish an ultra-fast liquid chromatography( UFLC) method for simultaneous determination of glycyrrhizin and glycyrrhizic acid in Glycyrrhiza uralensis Fisch. and its processed products,and provide references for its quality control. Methods Chromatographic separation was performed on Shim-Pack XR-ODS column(75 mm × 3. 0 mm,2. 2 μm). The mobile phase consisted of 0. 1% formic acid(A)-acetonitrile(B) with gradient elution(0 - 4 min,5% - 20% B;4 - 12 min 20% - 40% B;12 - 15 min,40% - 45% B) at a flow rate of 0. 8 mg/L and the equilibration time was 2 min. The detecting wavelength was set at 237 nm and column temperature was maintained at 30 ℃. Results The linear ranges were 10 - 500 mg/L( R = 0. 999 7) for glycyrrhizin and 10 - 500 mg/L( R =0. 999 8) for glycyrrhizic acid. The average recovery of glycyrrhizin and glycyrrhizic acid were 98. 9% and 99. 3%,respectively. Conclusion The developed method is simple,accurate with high repeatability and stability,which is suitable for the quality control of Glycyrrhiza uralensis Fisch.
作者 关皎 王强 孙宇慧 费雪 王黎明 郝乘仪 冯波 朱鹤云 GUAN Jiao ,WANG Qiang, SUN Yuhui, FEI Xue, WANG Liming, HAO Chengyi, FENG Bo, ZHU Heyun (College of Pharmacy, Jilin Medical University, Jilin City, Jilin Province, 132013, China)
出处 《吉林医药学院学报》 2018年第4期241-243,共3页 Journal of Jilin Medical College
基金 国家自然科学基金项目(81703683) 吉林省中医药管理局项目(2017098,2017250) 吉林省卫生计生委课题(2017Q048) 吉林市科技局杰出青年基金项目(20166029,20166031).
关键词 甘草 甘草苷 甘草酸 超快速液相色谱法 Glycyrrhiza uralensis Fisch. glycyrrhizin glycyrrhizic acid ultra fast liquid chromatography
作者简介 关皎(1983-),女(汉族),讲师,博士.;通讯作者:朱鹤云(1982-),男(汉族),副教授,博士.
  • 相关文献

参考文献12

二级参考文献78

共引文献110

同被引文献23

引证文献2

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈