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猪圆环病毒2型间接ELISA抗体检测试剂盒研制及应用 预览 被引量:1

Development and Application of Indirect ELISA Antibody Detection Kit for Porcine Circovirus 2
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摘要 以原核表达的猪圆环病毒2型Cap蛋白为包被抗原,建立间接ELISA方法并进行相应优化,成功研制猪圆环病毒2型间接ELISA抗体检测试剂盒,与国内市场标杆产品进行比较,敏感性为97.8%,特异性为92.8%,总体符合率达到了95%。同时,该试剂盒批间、批内变异系数均小于10%,具有较好的重复性。在临床应用中显示,该试剂盒测定江苏A猪场阳性率为82.2%,其中母猪阳性率为100%,育肥猪阳性率为73.3%。B猪场免疫母猪及后代的阳性率为98.3%,其中母猪阳性率为100%,后代为97.7%,且母猪的抗体水平高于子代,结果为圆环病毒2型在猪场的感染情况与疫苗免疫效果提供了有力的支撑。 Using the purified Cap recombinant protein as coating antigen,the indirect ELISA method was established and optimized for the ELISA test kit.And compared with the benchmarking products,the sensitivity,specificity and coincidence rate of the established ELISA method were 97.8%,92.8% and 95%.The variant coefficient of ELISA test kit in intra and interbatches were less than 10%.It had good reproducibility in the ELISA test kit.It showed 82.2% positive(sows 100% positive and offspring 73.3% positive)in farm A and 98.3%positive(sows 100%positive and offspring 97.7%positive)in farm B.The level of antibody of sows was higher than that of offspring in farm B.So it was helpful for detecting wild infection and antibody levels in vaccinated by the ELISA.
作者 李爽 荣桂芬 盛英霞 陈银辉 温凯 华利忠 熊雅婷 杨若松 LI Shuang 1 ,RONG Gui-fen 1 ,SHENG Ying-xia 2,CHEN Yin-hui 1,WEN Kai 1,HUA Li-zhong 3,XIONG Ya-ting 1,YANG Ruo-song 1(1.Peking Weideweikang Biological Technology Company,Beijing,100095,China;2.Shandong Animal Husbandry and Veterinary Information Center,Jinan,Shandong,250022,China;3.Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing,Jiangsu,210014,China)
出处 《动物医学进展》 北大核心 2018年第6期66-70,共5页 Progress In Veterinary Medicine
关键词 猪圆环病毒2型 ELISA试剂盒 符合率 临床应用 Porcine circovirus 2 ELISA test kit coincidence rate clinical application
作者简介 李爽(1990-)男,山东济宁人,硕士,主要从事动物传染病检测技术研究。同等贡献作者。;同等贡献作者。;通讯作者:杨若松
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