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不同种源黄芩(子代)药用成分含量的地理变异研究 被引量:1

Study on geographical variation of medicinal ingredients contents in Scutellaria baicalensis from different provenances
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摘要 为了研究不同种源黄芩(子代)药用成分含量的遗传差异,该研究通过随机区组设计布置黄芩种源试验,于生长3年后采挖药材,经统一干燥处理后,采用HPLC测定黄芩中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素和千层纸素A含量,并对数据进行分析。结果显示:(1)不同种源黄芩(子代)中所含黄芩苷含量具有显著差异(P〈0.05),而黄芩素的变异达到了极显著水平(P〈0.01);(2)聚类分析显示:以距离5.0为划分标准,河北承德地区种源可以划分为2个独立的种群,而其他2个种群地域跨度较大,说明黄芩的药用成分存在较为显著的地理变异。该研究为优质黄芩栽培的种源选择奠定了理论基础。 In order to investigate the genetic difference on medicinal components of Scutellaria baicalensis from different provenances on the genetic difference,the S. baicalensis provenance tests were arranged by randomized block design. Excavating the crude drugs that have been growing for three years,with the same drying process,the content of baicalin,baicalein,wogonoside,wogonin and laminarin A in S. baicalensis were detected by HPLC,and then the data were analyzed. The results indicated that the content of baicalin in different provenances of S. baicalensis was significantly different(P 0. 05),while the variation of baicalein reached extremely significant level(P 0. 01). Cluster analysis showed that if the distance was divided by 5. 0,the provenances in Chengde,Hebei province were divided into two independent populations,while the other two populations had large geographic spans. The results show that the significant geographical variations exist in the content of medicinal components in S. baicalensis. The study laid a theoretical foundation of provenance selection of S. baicalensis.
作者 李永杰 刘容秀 田文仓 王学森 侯志艳 孟磊 宋国虎 张学文 魏胜利 LI Yong-jie1, LIU Rong-xiu1, TIAN Wen-cang1 , WANG Xue-sen1, HOU Zhi-yan1, MENG Lei1 , SONG Guo-hu2, ZHANG Xue-wen2 , WEI Sheng-li1 ( 1. College of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China ; 2. Chengde Pharmaceutical Co. , Ltd. , Affiliated to China National Pharmaceutical Corporation, Chengde 067000)
出处 《中国中药杂志》 CSCD 北大核心 2018年第13期2664-2669,共6页 China Journal of Chinese Materia Medica
基金 国家“十二五”科技支撑计划项目(2011BAI07B04) 国家重点研发计划重点专项(2017YFC1701404) 国家中医药标准化项目(ZYBZH-Y-ZY-45)
关键词 黄芩 地理变异 种源选择 黄芩苷 Scutellaria baicalensis geographical variation provenance selection baicalin
作者简介 [通信作者]魏胜利,教授,硕士生导师,研究方向为中药资源定向培育及中药资源综合开发,Tel:(010)84738334,E-mail:wsl7491@126.com
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