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甘肃高山细毛羊和湖羊HIF-2α基因遗传特性与高原低氧适应性分析 预览 被引量:1

The Analysis of Gansu Alpine Merino and Hu Sheep HIF-2a Gene Genetic Characteristics and Adaptability to High Altitude Hypoxia
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摘要 为探讨HIF-2a基因核苷酸序列变异与绵羊高原适应的相关性,以540只(甘肃高山细毛羊200只,湖羊340只)绵羊为研究对象,采用PCR-SSCP技术分析HIF-2a基因第11外显子和内含子部分区段在甘肃高山细毛羊和湖羊中单核苷酸多态性(SNPs)。结果显示:HIF-2a基因扩增片段在2个绵羊品种中共检测到4种等位基因(A、B、C和D)和10种基因型(AA、BB、CC、DD、AB、AC、BC、AD、BD和CD),存在6个SNPs位点,其中外显子11检测到5个SNPs位点(G/A、G/C、C/T、T/C、G/A、),内含子11检测到1个SNPs位点(G/A),甘肃高山细毛羊未检测到DD基因型。2个绵羊品种的优势基因型均为AB,基因型频率分别为34.50%和16.47%。HIF-2a基因在2个绵羊品种中具有丰富的多态性,且等位基因频率在品种间存在极显著差异(P<0.01)。推测在高海拔低氧适应过程中,HIF-2a基因的基因型可能受到了选择,随着长期的高海拔低氧环境的选择,有利于低氧适应的基因型在高海拔绵羊品种中受到选择并富集,携带基因型AA、BB和AB的绵羊可能更加适应高海拔低氧环境,而携带基因型CD、BD和DD的绵羊对高海拔低氧环境适应的能力可能较差。 To study the relationship between the variations of HIF-2a gene and adaption to plateau in sheep,we detected the 540 sheep including 200 Gansu Alpin Merino and 340 Hu sheep,and used the PCR-SSCP to analyze the SNPs of part of the eleventh exon and intron of HIF-2a gene. The results showed that 6 SNPs corresponding to 4alleles( A,B,C and D) and 10 kinds of genotypes( AA,BB,CC,DD,AB,AC,BC,AD,BD and CD) were detected in HIF-2a gene in 2 sheep breed. The 5 SNPs existed in the eleventh exon and the one SNPs laid in the eleventh intron and the genotype DD was not detected in Gansu Alpine Merino. The advantaged genotype AB with frequency of0. 345 0 and 0. 164 7 was detected in both sheep breed. The HIF-2a gene has abundant polymorphism in 2 sheep breed and the frequency of alleles has significantly difference in two popul ations( P < 0. 01). As the selecting of environment with plateau and low oxygen,the HIF-2a gene was selected and clustered in plateu sheep. The sheep withthe genetype AA,BB and AB can adapt in plateau environment more better and the adaptive capacity of other sheep with genetype CD,BD and DD was poor.
作者 党鹏举 洪魏 胡江 罗玉柱 李少斌 王继卿 刘秀 DANG Pengju;LI Shaobin;WANG Jiqing;LIU Xiu;HONG Wei;HU Jiang;LUO Yuzhu;Gansu Key Laboratory of Herbivorous Animal Biotechnology,Gansu Engineering Lab of Genetic Improvement in Runminants,Faculty of Animal Science and Technology,Gansu Agricultural University;Zhejiang Sainuo Ecological Agriculture Limited Company;
出处 《华北农学报》 CSCD 北大核心 2016年第3期88-93,共6页 Acta Agriculturae Boreali-Sinica
基金 甘肃省国际科技合作专项(1304WCGA17S) 甘肃农业大学盛彤笙科技创新基金项目(GSAU-STS-134) 甘肃省青年科技基金计划项目(130SRJYA025) 甘肃省髙等学校科研项目(2015B-052)
关键词 HIF-2a基因 甘肃高山细毛羊 适应性 多态性 HIF-2α gene Gansu Alpine Merino Adaptability Polymorphism
作者简介 党鹏举(1987-),男,甘肃定西人,硕士,主要从事生物技术与动物育种研究。;通讯作者:罗玉柱( 1962-) ,男,甘肃景泰人,教授,博士,博士生导师,主要从事现代生物技术与动物育种应用研究。
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