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免疫亲和柱—超高效液相色谱法测定维生素饮料中维生素B12 预览 被引量:1

The Determination of Vitamin B12 in Drinks with Immune Affinity Column-Ultra High Performance Liquid Chromatography
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摘要 采用免疫亲和柱—超高效色谱法建立维生素饮料中维生素B 12的测定方法,样品经免疫亲和柱富集、净化后,用3mL甲醇以流速为1滴/秒的速度洗脱,氮气吹干后溶解再经超高效液相色谱法测定。色谱柱为HSS T3,柱温35℃,流动相为乙腈-0.1%甲酸溶液梯度洗脱,流速为0.6mL/min,波长为360nm测定。结果表明,维生素B 12在浓度为0.4~10μg/L内线性关系良好,回归方程为Y=4 040X-364,相关系数为0.997 00,回收率为94.68%,检出限为0.1μg/L。本方法具有操作简单、灵敏度高、结果准确、检测效率高等优点,满足于成分复杂、维生素含量低的维生素饮料中B 12的测定要求。 Super efficient immune affinity column chromatography was adopted to establish the method for determination of the vitamin B 12 in vitamin drink,sample after immune affinity column,purification,with 3 mL methanol elution at a speed of flow rate of 1 drop/Sec,nitrogen blow dry after dissolved by Ultra High Performance Liquid Chromatography(UPLC)method.Chromatographic column for HSS T3,column temperature 35℃,mobile phase gradient elution of acetonitrile-0.1%formic acid solution,flow rate of 0.6 mL/min,the wavelength of 360 nm.Results showed that the concentration of vitamin B 12 in the 0.4~10μg/L range inside sex was good,the regression equation of y=4 040 x-4 040,the correlation coefficient was 0.997 00,recovery rate was 94.68%,the detection limit was 0.1μg/L.This method has the advantages of simple operation,high sensitivity,accurate,high detection efficiency,and is suitable for the determination of vitamin B 12 in drinks with complex composition and low vitamin content.
出处 《中国食物与营养》 2018年第3期27-29,共3页 Food and Nutrition in China
关键词 维生素B12 维生素饮料 免疫亲和柱 超高效液相色谱 vitamin B12 vitamin drink immune affinity column Ultra High Performance Liquid Chromatography(UPLC)
作者简介 周黎(1982— ),女,硕士,研究方向:食品分析及安全。
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