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紫花山奈的组培快繁研究 预览

In vitro rapid propagation of Kaempferia elegans
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摘要 以紫花山柰无菌苗的芽基部为外植体,对芽基部进行继代增殖、丛生芽诱导以及生根培养基的筛选,建立了紫花山柰无菌苗芽基部的组培快繁体系。结果表明:紫花山柰无菌苗芽基部继代增殖比较合适的培养基为MS+6-BA8~12mg/L+NAA0~0.1mg/L+椰汁10%+白砂糖30g/L+卡拉胶粉10g/L,最佳继代增殖培养基为MS+6-BA8mg/L+NAA0.1mg/L+椰汁10%+白砂糖30g/L+卡拉胶粉10g/L;丛生芽诱导较合适的培养基为MS+6-BA3~5mg/L+NAA0.05~0.1mg/L+椰汁10%+白砂糖30g/L+卡拉胶粉10g/L,最佳的丛生芽诱导培养基为MS+6-BA3mg/L+NAA0.05mg/L+椰汁10%+白砂糖30g/L+卡拉胶粉10g/L;生根较佳的培养基为MS+NAA0.1mg/L+香蕉泥10~15%+活性炭0.5~1.0g/L+白砂糖30g/L+卡拉胶粉9.5g/L。 The shoot bases of Kaempferia elegans sterile seedlings were used as explants which were subcultured for propagation,induced for multiple shoots,and rooted to produce rooted plants,and their medium for tissue culture were screened to establish a rapid propagation system for K.elegans.The results showed that the appropriate medium for propagation of K.elegans were MS+6-BA 8~12 mg/L+NAA 0~0.1 mg/L+coconut milk 10%+sucrose 30 g/L+cara rubber powder 10 g/L,and the best propagation medium was MS+6-BA 8 mg/L+NAA 0.1 mg/L+coconut milk 10%+sucrose 30 g/L+cara rubber powder 10 g/L.The suitable medium for inducing multiple shoots were MS+6-BA 3~5 mg/L+NAA 0.05~0.1 mg/L+coconut milk 10%+sucrose 30 g/L+cara rubber powder 10 g/L,and the most suitable medium for inducing multiple shoots was MS+6-BA 3 mg/L+NAA 0.05 mg/L+coconut milk 10%+sucrose 30 g/L+cara rubber powder 10 g/L.The optimum rooting medium was MS+NAA 0.1 mg/L+mashed banana 10~15%+activated carbon 0.5~1.0 g/L+sucrose 30 g/L+cara rubber powder 9.5 g/L.
作者 吴满良 李冬梅 朱根发 刘小飞 WU Man-liang[1];LI Dong-mei[1];ZHU Gen-fa[1];LIU Xiao-fei[1]([1]Environmental Horticulture Research Institute,Guangdong Academy of Agricultural Sciences,Guangdong Key Lab of Ornamental Plant Germplasm Innovation and Utilization,Guangzhou 510640,China)
出处 《广东农业科学》 CAS 2018年第7期29-33,173共6页 Guangdong Agricultural Sciences
基金 广东省科技计划项目(2015A020209078) 广州市科技计划项目(201607010101)。
关键词 紫花山奈 组织培养 芽基部 增殖 芽诱导 生根 Kaempferia elegans tissue culture bud base propagation bud induction rooting
作者简介 吴满良(1995-),男,在读本科生,E-mail:349661183@qq.com。;通讯作者:李冬梅(1980-),女,博士,副研究员,E-mail:740735290@qq.com。
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