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反相高效液相色谱法同时测定党参阿胶丸4种成分的含量

Simultaneous Determination of Four Components in Codonopsis Radix Colla Corii Asini Pill by RP-HPLC
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摘要 目的 建立同时测定党参阿胶丸中阿魏酸、毛蕊异黄酮葡萄糖苷、甘草苷和甘草酸的反相高效液相色谱法。方法 采用Inert Sustain C 18色谱柱(4.6 mm×250 mm,5μm),0.2%甲酸溶液为流动相A,乙腈为流动相B,梯度洗脱,检测波长为237 nm,柱温:30℃,流速:1.0 mL·min-1,进样量:20μL。结果 阿魏酸、毛蕊异黄酮葡萄糖苷、甘草苷和甘草酸分别在1.25~62.47μg·mL-1(r=1.0000)、4.84~241.85μg·mL-1(r=1.0000)、1.95~97.66μg·mL-1(r=0.9999)、18.82~941.16μg·mL-1(r=0.9999)线性关系良好,平均加样回收率(n=6)分别为103.5%,88.73%,93.77%,100.2%;RSD分别为5.9%,9.5%,7.8%,9.5%。结论 新建反相高效液相色谱梯度法可同时测定党参阿胶丸中阿魏酸,毛蕊异黄酮葡萄糖苷,甘草苷和甘草酸的含量。该方法准确、可行,为进一步修订党参阿胶丸质量标准提供可靠方法。 Objective To establish an RP-HPLC method forsimultaneous determination of ferulic acid,calycosin-7-glucosid,liquiritin and glycyrrhizic acid in Codonopsis Radix Colla Corii Asini Pill(CCP).Methods The experiments were performed on a Inert Sustain C 18 column(4.6 mm×250 mm,5μm)with a gradient mobile phase of 0.5%formic acid(A)and acetonitrile(B).The detection wavelength was 237 nm,the column temperature was 30℃,the flow rate was 1.0 mL·min-1 and the injection volume was 20μL.Results The 4 constituents showed a good linear relationship within the range of of 1.25-62.47μg·mL-1(r=1.0000)for ferulic acid,4.84-241.85μg·mL-1(r=1.0000)for calycosin-7-glucosid,1.95-97.66μg·mL-1(r=0.9999)for liquiritin,18.82-941.16μg·mL-1(r=0.9999)for glycyrrhizic acid.The average recovery was 103.5%,88.73%,93.77%,100.2%with the RSDs of 5.9%,9.5%,7.8%and 9.5%(n=6),respectively.Conclusion The experiment establishes an RP-HPLC method for simultaneous determination of ferulic acid,calycosin-7-glucosid,liquiritin and glycyrrhizic acid in CCP.It also provides a simple and effective methodto further revise the quality standard of CCP.
作者 刘婷 何开勇 兰静 宋丹青 LIU Ting;HE Kaiyong;LAN Jing;SONG Danqing(Institute of Medicinal Biotechnology,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China;National Institutes for Food and Drug Control,Beijing 100050,China;Hubei Institute for Drug Control,Wuhan 430075,China;Beijing Xinlan Pharmatech Co.,Ltd,Beijing 100166,China)
出处 《医药导报》 CAS 北大核心 2018年第10期1251-1255,共5页 Herald of Medicine
关键词 党参阿胶丸 阿魏酸 毛蕊异黄酮葡萄糖苷 甘草苷 甘草酸 色谱法 高效液相 反相 Radix Colla Corii Asini pill Ferulic acid Calycosin-7-glucosid Liquiritin Glycyrrhizic acid RP-HPLC
作者简介 刘婷(1984-),女,河北蔚县人,助理研究员,在读博士,主要从事药品、食品、保健品和化妆品质量控制研究。电话:010-67095155,E-mail:lutyliu@126.com;通信作者,宋丹青(1964-),女,山东青岛人,教授,博士生导师,主要从事微生物与生化药学研究。电话:010-63165268,E-mail:songdanqingsdq@sina.com。
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