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TLR4/NF-κB表达升高促进宫颈癌增殖和转移的作用及机制 预览

Effect and mechanism of TLR4/NF-κB signaling pathway on proliferation and metastasis of cervical cancer
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摘要 目的研究Toll样受体4(Toll-likeReceptor4,TLR-4)/核因子-κB(nuclear factor-kappaB,NF-κB)分子在宫颈癌中的表达,探讨TLR4/NF-κB表达与宫颈癌增殖、迁移的关系及具体机制。方法采用实时定量PCR(quantitative real-timePCR,qRT-PCR)比较宫颈癌组织及癌旁组织中TLR4/NF-κB的表达。采用脂质体介导转染方法,根据人宫颈癌细胞株HCE1细胞TLR4表达情况,分为Blank、阴性对照、TLR4沉默、TLR4过表达4组。采用CCK8、划痕实验、Transwell实验检测TLR4对细胞增殖、迁移、侵袭,并进一步检测Myd88,ERK1/2及NF-κB信号分子改变。结果肿瘤组织TLR4/NF-κB的表达显著高于癌旁组织(P<0.05);宫颈癌细胞株HCE1中TLR4/NF-κB表达显著高于正常宫颈上皮细胞(P<0.05)。TLR4沉默组细胞增殖能力较NC组显著下降(P<0.05),过表达TLR4细胞增殖能力显著升高(P<0.05)。TLR4沉默组愈合面积比例显著低于NC组(P<0.05),过表达TLR4组愈合面积比例显著升高(P<0.05)。TLR4沉默组穿膜细胞数明显少于NC组(P<0.05),过表达TLR4组穿膜细胞数明显多于NC组(P<0.05)。TLR4沉默组Myd88,ERK1/2及NF-κB的表达显著低于NC组;TLR4过表达组Myd88,ERK1/2及NF-κB的蛋白表达显著高于NC组(P均<0.05)。结论TLR4/NF-κB在宫颈癌中表达升高,促进宫颈癌细胞增殖、迁移、侵袭。 Objective To study the expression of Toll-like Receptor4(TLR4)/nuclear factor-kappa B(NF-κB)in cervical cancer,and to explore the relationship between TLR4/NF-κB expression with proliferation and migration of cervical cancer and its mechanism.Methods Real time quantitative PCR was used to compare the expression of TLR4/NF-κB in cervical cancer tissues and adjacent tissues.The expression of TLR4in human cervical cancer cell line HCE1was silenced or over-expressed by liposome-mediated transfection.The effect of TLR4on cell proliferation,migration,invasiveness ability was detected by CCK8,scratch test and Transwell assay.The changes of Myd88,ERK1/2and NF-κB signal molecules were further detected.Results The expression of TLR4/NF-κB in tumor tissue was significantly higher than that of adjacent tissue(P<0.05),and the relative expression of TLR4/NF-κB in HCE1was significantly higher than that of normal cervical epithelial cells(P<0.05).The cell proliferation ability of TLR4silencing group was significantly lower than that of NC group(P<0.05),while the ability in TLR4overexpressed cells was significantly higher(P<0.05).The proportion of healing area in TLR4silencing group was significantly lower than that in NC group(P<0.05),while the proportion in TLR4overexpressed group was significantly increased(P<0.05).The number of penetrating cells in TLR4silencing group was significantly less than that in group NC(P<0.05),while the number in TLR4overexpressed group was significantly higher(P<0.05).The expression of Myd88,ERK1/2and NF-κB in TLR4silencing group was significantly lower than that in NC group,while their expression in TLR4overexpressed group was significantly higher(P<0.05).Conclusion Upregulated TLR4/NF-κB activation could promote the proliferation,migration and invasion of cervical cancer.
作者 蔡静 张丹 张燕 CAI Jing;ZHANG Dan;ZHANG Yan(The department of Obstetrics and Gynecology,Wuhan Children′s Hospital(Wuhan Maternal and Child Healthcare Hospital),Tongji Medical College,Huazhong University of Science &Technology, Wuhan 430015, China)
出处 《中国生育健康杂志》 2019年第1期21-25,共5页 Chinese JOurnal of Reproductive Health
基金 湖北省卫生计生委重点支撑项目 (WJ2017Z002).
关键词 TLR4 NF-ΚB 宫颈癌 侵袭 迁移 增殖 病理分级 TLR4 NF-κB cervical cancer invasion migration proliferation pathological grading
作者简介 通讯作者:张燕(zyfck@sina.com)
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