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青海高原耐旱蚕豆品种青海13号响应干旱胁迫蛋白质组学分析 预览

Proteomic analysis of drought stress response on drought resistance for Vicia faba L.variety'Qinghai 13'in Qinghai Plateau of China
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摘要 蛋白质组学研究在功能基因组时代发挥着越来越重要的作用,利用双向电泳技术和质谱鉴定技术,可大量研究作物逆境胁迫后蛋白质组的变化,增加作物响应干旱胁迫机制的认识和理解。为探讨一种抗旱性蚕豆青海13号品种的耐旱机制,本研究对其幼苗期进行干旱胁迫处理,应用上述技术进行差异蛋白质组分析,经t检验发现32个差异表达蛋白点,部分呈现上调表达,部分呈现下调表达,还有7个消失蛋白点和1个新增蛋白点。采用MALDI-TOF/TOF鉴定和生物信息学分析发现,成功鉴定的21个蛋白点按其所参与的代谢途径和生化功能可分为七大类,参与信号转导的2个,参与自由基清除的1个,参与防卫反应的1个,参与代谢的8个,参与蛋白加工的1个,参与光合的5个,未知功能蛋白3个。22kD干旱诱导蛋白、应激诱导蛋白、17.5kD一级热激蛋白、超氧化物歧化酶是与抗旱性有直接关联的蛋白点,其相对表达量的上调可能是青海13号蚕豆具有较强抗旱性的重要原因。 Proteomics is playing an increasingly important role in the functional genomics era. Two-dimensional electrophoresis and mass spectrometry can be used to study the changes of proteome of crop under stress and increase the recognization and comprehension of crop response to drought stress. In order to explore the mechanism of drought resistance of Vicia faba L. variety ‘Qinghai 13’, we treated the seedlings with three days for water stress and analyzed by two-dimensional gel electrophoresis combined with mass spectrometry analysis. By t-test, 32 differentially expressed proteins spots were detected between normal and drought-stress treatments, respectively, including up-and down-regulated proteins, seven disappear protein spots and a new protein spot. Twenty-one differentially expressed proteins in seven function categories were identified and confirmed by MALDI-TOF/TOF. Among them, two participated in information transfer, one in oxygen radical scavenging, one in protective response, eight in energy metabolism, one in protein processing, five in photosynthesis, and three unknown in function. These results indicate that drought-inducible 22 kD protein, stress-inducible protein, superoxide dismutase and 17.5 kD class HSP are directly related with drought resistance, which may be the important reasons for strong drought resistance of ‘Qinghai 13’.
作者 李萍 侯万伟 刘玉皎 LI Ping;HOU Wan-Wei;LIU Yu-Jiao(Academy of Agriculture and Forestry Science of Qinghai University,Xining 810016,Qinghai,China;State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University,Xining 810016,Qinghai,China)
出处 《作物学报》 CAS CSCD 北大核心 2019年第2期267-275,共9页 Acta Agronomica Sinica
基金 国家自然科学基金项目(31460377) 国家现代农业产业技术体系建设专项(CARS-09)资助。
关键词 蚕豆 干旱胁迫 蛋白质组 差异表达 功能分类 faba bean drought stress proteomic differential expression functional classification
作者简介 第一作者联系方式:李萍,E-mail:lping2008@sohu.com;通信作者:刘玉皎,E-mail:13997058356@163.com
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