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不同干燥方法对麦冬多元活性成分的影响 预览

Effects of different drying methods on multiple bioactive constituents of Ophiopogonis Radix
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摘要 探究不同干燥方法对麦冬多元活性成分的影响。采用超快速液相色谱-三重四极杆/线性离子阱质谱(UFLC-QTRAP-MS/MS)同时测定不同干燥方法下麦冬中甾体皂苷、高异黄酮、氨基酸及核苷类共29种活性成分的含量;根据29种目标成分的含量,用聚类分析(HCA)及主成分分析(PCA)对不同干燥方法下麦冬进行综合评价。结果表明,29种目标成分在一定浓度范围内线性关系良好,相关系数均大于0.9990;精密度、重复性、稳定性良好,RSD均小于5%;平均加样回收率为97.02%~102.78%,RSD均小于5%。PCA结果显示,以晒干、无硫烘干的麦冬样品综合质量较优。所建立的方法准确、可靠,可用于麦冬药材内在质量的综合评价,该研究可为揭示麦冬传统干燥方法科学内涵提供依据,同时为麦冬产地加工时干燥方法的优选提供基础资料。 To explore the effects of different drying methods on multiple bioactive constituents in Ophiopogonis Radix.A comprehensive analytical method employed with ultra-fast liquid chromatography tandem triple quadrupole mass spectrometry(UFLC-QTRAP-MS/MS)was used for the simultaneous determination of twenty-nine components including steroidal saponins,homoisoflavonoids,amino acids and nucleosides in Ophiopogonis Radix.Hierarchical clustering analysis(HCA)and principal component analysis(PCA)have been applied to comprehensively analyze and evaluate the results of multiple active constituents.The results showed that twenty-nine components had good linearity,and correlation coefficients were more than 0.9990.The method exhibited good precision,repeatability and stability with the relative standard deviation(RSD)less than 5%,the average recoveries were between 97.02% and 102.78%,and all RSD were less than 5%.In addition,the results of PCA showed that sun drying and non-sulfurized oven drying samples have better quality.The established method was accurate and reliable,which could be used to appraise the quality of Ophiopogonis Radix.Our study can provide basis for revealing the scientific connotation of traditional drying method of Ophiopogonis Radix,and may lay the way for optimization of drying method during the processing of Ophiopogonis Radix.
作者 谈梦霞 陈佳丽 邹立思 刘训红 唐仁茂 马继梅 陈舒妤 石婧婧 TAN Meng-xia;CHEN Jia-li;ZOU Li-si;LIU Xun-hong;TANG Ren-mao;MA Ji-mei;CHEN Shu-yu;SHI Jing-jing(School of Pharmacy,Nanjing University of Chinese Medicine,Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing 210023,China;;SZYY Group Pharmaceutical Limited,Taizhou 225500,China)
出处 《天然产物研究与开发》 CAS CSCD 北大核心 2019年第1期100-109,共10页 Natural Product Research and Development
基金 国家中药标准化项目(ZYBZH-C-JS-32) 江苏高校优势学科建设工程(YSXK-2014) 江苏高校品牌专业建设工程(PPZY2015A070)。
关键词 麦冬 干燥方法 超快速液相色谱-三重四极杆/线性离子阱质谱 多元活性成分 聚类分析 主成分分析 Ophiopogonis Radix drying method UFLC-QTRAP-MS/MS multiple bioactive constituents hierarchical clustering analysis principal component analysis
作者简介 通信作者:刘训红,Tel:86-25-85811511;E-mail:liuxunh1959@163.com
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