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槲寄生多糖调控胃癌细胞增殖、迁移和侵袭的机制研究 预览

Mechanism of Viscum coloratum polysaccharide to regulate proliferation,migration,and invasion of gastric cancer cells
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摘要 背景胃癌(gastric cancer,GC)是严重危害人体健康的恶性肿瘤,其发病率和死亡率分别占世界恶性肿瘤的第5位和第3位.中医药治疗能够减轻患者病痛,改善术后复发,槲寄生多糖(viscum coloratum polysaccharide,VCP)是槲寄生抗肿瘤的主要活性成分之一,能够抑制癌细胞增殖并诱导凋亡.目的观察VCP对胃癌细胞增殖、迁移和侵袭能力的影响并探讨其潜在作用机制.方法以不同浓度(20μg/mL、40μg/mL、60μg/mL、80μg/mL、100μg/mL)VCP干预SGC-7901人GC细胞48 h,MTT法检测细胞活力.以100μg/mLVCP干预SGC-7901细胞,Western blot、Transwell小室分别检测细胞中周期蛋白依赖性蛋白激酶4(cyclin-dependent protein kinases 4,CDK4)、基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)、基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)和核转录因子κB p65蛋白表达及细胞迁移和侵袭能力变化.结果不同浓度VCP均可抑制SGC-7901细胞活力(P<0.05),呈浓度依赖性.Western blot和Transwell实验结果表明:与对照组相比,100μg/mL VCP处理的SGC-7901细胞中CDK4、MMP-2、MMP-9、核转录因子-κB(nuclear factor kappa beta,NF-κB)p65蛋白表达下调(P<0.05),同时视野内迁移和侵袭细胞数明显减少(P<0.05).结论VCP对SGC-7901人GC细胞增殖、迁移和侵袭具有抑制作用,其机制可能与NF-κB信号通路及CDK4、MMP-2和MMP-9蛋白有关. BACKGROUND Gastric cancer(GC)is the fifth most common cancer globally and the third leading cause of cancer death.Traditional Chinese medicine treatment can alleviate patients’pain and improve postoperative recurrence.Viscum coloratum polysaccharide is one of the main antitumor active components of Viscum coloratum and can inhibit proliferation and induce apoptosis of cancer cells.AIM To investigate the effect of Viscum coloratum polysaccharide on the proliferation,migration,and invasion of human gastric cancer cells(SGC-7901)and the underlying mechanism.METHODS Viability of SGC-7901 cells treated with Viscum coloratum polysaccharide at 20μg/mL,40μg/mL,60μg/mL,80μg/mL,and 100μg/mL for 48 h was detected by MTT assay.Cell migration and invasion were detected by Transwell assays,and the protein expression of cyclin-dependent kinases 4(CDK4),matrix metalloproteinase 2(MMP-2),matrix metalloproteinase 9(MMP-9),and nuclear factorκB(NF-κB)p65 in SGC-7901 cells was detected by Western blot.RESULTS Different concentrations of Viscum coloratum polysaccharide could inhibit the viability of SGC-7901 cells in a dose-dependent manner(P<0.05).The results of Western blot indicted that the protein expression of CDK4,MMP-2,MMP-9,and NF-κB p65 in SGC-7901 cells treated with 100μg/mL Viscum coloratum polysaccharide was down-regulated(P<0.05),and Transwell assays showed that the numbers of migratory and invaded cells in the visual field were significantly decreased(P<0.05).CONCLUSION Viscum coloratum polysaccharide may inhibit the proliferation,migration,and invasion of SGC-7901 cells by regulating the NF-κB signaling pathway and the expression of CDK4,MMP-2,and MMP-9 proteins.
作者 宣平 李子芳 周亮 李厥宝 Ping Xuan;Zi-Fang Li;Liang Zhou;Jue-Bao Li(Department of Rehabilitation Medicine,Yiwu Central Hospital,Yiwu 322000,Zhejiang Province,China;Department of Rehabilitation Medicine,Zhejiang People’s Hospital,Hangzhou 310000,Zhejiang Province,China)
出处 《世界华人消化杂志》 CAS 2019年第2期80-86,共7页 World Chinese Journal of Digestology
关键词 槲寄生多糖 胃癌 迁移 侵袭 Viscum coloratum polysaccharide Gastric cancer Migration Invasion
作者简介 通讯作者:宣平,住院医师,研究方向为神经康复以及骨关节康复.322000,浙江省义乌市南门街519号,义乌市中心医院 康复医学科.xuqqxing@sina.com
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