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HPLC-DAD法同时测定蒙药森登-4汤散中7种抗风湿活性成分的含量 预览

Simultaneous Determination of 7 Anti-rheumatic Active Ingredients in Mongolian Medicine Sendeng-4 Decoction Powder by HPLC-DAD
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摘要 目的:建立同时测定蒙药森登-4汤散中7种抗风湿活性成分儿茶素、栀子苷、二氢杨梅素、花旗松素、芦丁、杨梅素和槲皮素含量的方法。方法:采用高效液相色谱-二极管阵列检测法(HPLC-DAD)。色谱柱为Sil Green C18,流动相为乙腈-0.1%磷酸溶液(梯度洗脱),流速为1mL/min,检测波长为238nm,柱温为30℃,进样量为10μL。结果:儿茶素、栀子苷、二氢杨梅素、花旗松素、芦丁、杨梅素和槲皮素检测质量浓度的线性范围分别为8.590~2290、10.56~3901、13.00~3958、8.815~564.2、4.030~257.8、8.130~750.5、7.075~454.2μg/mL(r≥0.9991);检测限分别为0.4295、0.2640、0.3250、0.2204、0.2015、0.2032、0.1769μg/mL,定量限分别为1.030、1.321、1.302、1.397、1.637、0.8130、0.7075μg/mL;精密度、稳定性(48h)、重复性试验的RSD均小于2.0%(n=6);平均加样回收率分别为96.24%~99.28%,RSD分别为1.03%~1.63%(n=6)。结论:该方法简便、准确、重复性良好,可用于同时测定蒙药森登-4汤散中儿茶素、栀子苷、二氢杨梅素、花旗松素、芦丁、杨梅素和槲皮素的含量。 OBJECTIVE:To establish a method for simultaneous determination of 7 kinds of anti-rheumatic active ingredients in Mongolian medicine Sendeng-4 decoction powder,such as catechin,jasminoidin,dihydromyricetin,texifolin,rutin,myricetin and quercetin.METHODS:HPLC-DAD method was adopted.The determination was performed on Sil Green C18 column with mobile phase consisted of acetonitrile-0.1%phosphoric acid solution(gradient elution)at the flow rate of 1 mL/min.The detection wavelength was set at 238 nm,and column temperature was 30℃.The sample size was 10μL.RESULTS:The linear range of catechin,jasminoidin,dihydromyricetin,texifolin,rutin,myricetin and quercetin were 8.590-2 290,10.56-3 901,13.00-3 958,8.815-564.2,4.030-257.8,8.130-750.5,7.075-454.2μg/mL(r≥0.999 1),respectively;the limits of detection were 0.429 5,0.264 0,0.325 0,0.220 4,0.201 5,0.203 2,0.176 9μg/mL,respectively;the limits of quantification were 1.030,1.321,1.302,1.397,1.637,0.813 0,0.707 5μg/mL,respectively.RSDs of precision,stability(48 h)and repetition tests were all lower than 2.0%(n=6).The average recoveries were 96.24%-99.28%(RSD=1.03%-1.63%,n=6).CONCLUSIONS:The established method is simple,accurate and reproducible,and can be used for simultaneous determination of catechin,jasminoidin,dihydromyricetin,texifolin,rutin,myricetin and quercetin in Mongolian medicine Sendeng-4 decoction powder.
作者 苏日塔拉图 于佳琦 王曦烨 刘景林 徐宁 许良 Suritalatu;YU Jiaqi;WANG Xiye;LIU Jinglin;XU Ning;XU Liang(Dept. of Mongolian Medicine Preparation,the Affiliated Hospital of Inner Mongolia University for the Nationalities,Inner Mongolia Tongliao 028042,China;College of Chemistry and Chemical Engineering/Inner Mongolia Key Laboratory of Natural Products Chemistry and Functional Molecules Synthesis,Inner Mongolia University for Nationalities,Inner Mongolia Tongliao 028042,China)
出处 《中国药房》 CAS 北大核心 2019年第2期221-225,共5页 China Pharmacy
基金 国家自然科学基金资助项目(No.30760299、81260682、81560702).
关键词 森登-4 汤散 高效液相色谱-二极管阵列检测法 儿茶素 二氢杨梅素 花旗松素 栀子苷 芦丁 杨梅素 槲皮素 含量 Sendeng-4 decoction powder HPLC-DAD Catechin Dihydromyricetin Texifolin Jasminoidin Rutin Myricetin Quercetin Content
作者简介 苏日塔拉图,副主任蒙药师。研究方向:蒙药制剂。电话:0475-8267801。E-mail:Suri.tala@163.com;通信作者:许良,教授,博士生导师,博士。研究方向:蒙药标准化及蒙药炮制方法。电话:0475-8313484。E-mail:nmgxl66@163.com
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