期刊文献+

2011-2015年环洞庭湖区H3亚型禽流感病毒的分离鉴定与遗传演化 预览

Isolation,Identification and Genetic Evolution of H3 Subtype Avian Influenza Virus in Dongting Lake Region from 2011 to 2015
在线阅读 免费下载
分享 导出
摘要 为了解环洞庭湖地区家禽H3亚型禽流感病毒的感染情况和进化规律,笔者对2011-2015年湖南省环洞庭湖地区的多个活禽市场与散养鸭场分离的31株H3亚型禽流感病毒进行基因测序和进化分析。结果表明:所有病毒的HA裂解位点不含连续的碱性氨基酸,为低致病性禽流感病毒的分子特征;各基因片段进化树显示部分病毒的PA与PB2基因与H5在相同分支,部分病毒的NP、PA、PB1与H7处于相同分支,表明H3可能与H5、H7亚型的禽流感病毒发生了复杂的基因交换;散养家禽分离的部分H3亚型禽流感病毒内部基因与越南、韩国等周边国家野鸟源的H3、H6、H7、H11等亚型同源性较高,可能有相同的进化来源。进一步将分离的病毒进行全基因比对,可划分出21种不同的病毒基因型。环洞庭湖地区H3亚型禽流感病毒基因来源复杂,表现出明显的遗传多样性。 To better understand the situation and evolution of poultry infected with H3 subtype avian influenza viruses around Dongting Lake,active monitoring at live-poultry markets and Free-range duck farms at this region were implemented in 2011-2015,and a total of 31 strains of H3 subtype AIV were isolated.Gene sequencing and evolutionary analysis of these strains were conducted in our study.Sequencing results showed that all the viruses were located in the Eurasian lineage except for two H3N8s in North America lineage,all isolates were low pathogenic avian influenza virus.Phylogenetic tree showed that PA and PB2 genes of some viruses were clustered with H5,while NP,PA and PB1 were clustered with H7,suggesting that complicated gene exchange were occurred.Internal genes of some viruses isolated from free-range poultry show very high homology with wild birds origin H3,H7,H11 from neighboring countries such as Vietnam and South Korea,may share the same evolutionary origin.Further genome sequence alignment can divided all viruses into 21 different genotypes.The source of H3 subtype avian influenza virus in the Dongting Lake area is complicated,showing a clear genetic diversity.
作者 何世成 彭志 王卫国 唐小明 鲁杏华 林源 邓国华 王昌建 HE Shicheng;PENG Zhi;WANG Weiguo;TANG Xiaoming;LU Xinghua;LIN Yuan;DENG Guohua;WANG Changjian(Hunan Animal Disease Prevention and Control Center,Changsha 410014,China;Animal Influenza Key Laboratory of the Ministry of Agriculture,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150069,China)
出处 《畜牧兽医学报》 CAS CSCD 北大核心 2019年第2期382-389,共8页 Chinese Journal of Animal and Veterinary Sciences
基金 "十三五"重点研发计划项目(2016YFD0500201).
关键词 洞庭湖区 H3亚型禽流感病毒 进化分析 基因型 Dongting Lake H3 subtype avian influenza virus evolutionary analysis genotype
作者简介 通信作者:彭志,主要从事预防兽医学研究,E-mail:807701751@qq.com;通信作者:邓国华,主要从事动物病毒学及其分子生物学研究,E-mail:dgh1971@163.com.
  • 相关文献

参考文献2

二级参考文献29

共引文献9

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈