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不同ELISA试剂盒检测感染猪口蹄疫病毒3ABC抗体效果比较 预览

Analysis of FMDV Infected Swine Non-structural Protein Antibody with Different Kits
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摘要 为更准确研究口蹄疫病毒(FMDV)感染猪非结构蛋白抗体变化规律,选取3种不同的试剂盒,分别检测接种O型口蹄疫病毒猪FMDV非结构蛋白3ABC抗体。试验数据表明,人工感染O型口蹄疫病毒猪最早在感染后第5天检测到非结构蛋白抗体,至第7天3ABC抗体全部转为阳性;3种试剂盒检测最早3ABC抗体与症状对应关系有差异,每两种商业试剂盒呈现不同程度的相关性。其中A试剂盒显示出最大的灵敏度,而B试剂盒显示最高的特异性,C试剂盒的敏感性依可疑样品的处理方式而变化,因此对3种试剂盒进行比较,发现A试剂盒更稳定,更适合于猪FMDV非结构蛋白抗体检测。 In order to study the change rule of non-structural protein antibodies in pigs inoculated with foot-and-mouth disease virus,three different kits were used in this study.The change rule of FMDV non-structural protein 3ABC antibodies in pigs inoculated with type O foot-and-mouth disease virus were detected by ELISA.The experimental data showed that pigs inoculated with type O foot-and-mouth disease virus firstly detected positive on the 5th day after infection,and all 3ABC antibodies became positive on the 7th day;non-structural protein antibody response and symptoms were different,and each two of commercial kits showed different degrees of correlation.Among them,the A kit showed the greatest sensitivity,while the B kit showed the greatest specificity,and the sensitivity of the C kit varied depending on the process of suspicious samples.Therefore,comparison the 3 kits revealed that the A kit was more stable and more suitable for detecting FMDV non-structural protein antibodies in pigs.
作者 王文莉 刘华南 卢炳州 赵俊皓 徐守兴 胡永浩 郑海学 张克山 刘湘涛 WANG Wen-li;LIU Hua-nan;LU Bing-zhou;ZHAO Jun-hao;XU Shou-xing;HU Yong-hao;ZHENG Hai-xue;ZHANG Ke-shan;LIU Xiang-tao(Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Animal Virology,Ministry of Agriculture National Foot and Mouth Disease Reference Laboratory,Lanzhou,Gansu,730046,China;College of Veterinary Medicine,Gansu Agricultural University,Lanzhou,Gansu,730070,China)
出处 《动物医学进展》 北大核心 2019年第2期39-44,共6页 Progress In Veterinary Medicine
基金 国家科技支撑计划项目(2015BAD12B04) 国家自然科学基金-联合基金项目(U1501213).
关键词 口蹄疫病毒 3ABC抗体 酶联免疫吸附试验 Foot-and-mouth disease virus 3ABC antibody ELISA pig
作者简介 王文莉(1988-),女,甘肃景泰人,兽医师,硕士,主要从事口蹄疫病毒蛋白质组学研究;通讯作者:张克山.
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