[目的]探讨microRNA-506 (miR-506)在绒毛膜癌中对绒毛膜癌细胞的作用及其作用机制。[方法]通过qRT-PCR检测绒毛膜癌和正常早孕绒毛临床标本中miR-506及caveolin-1表达量及相关性;将绒毛膜癌细胞JEG3和JAR按照miR-506 mimic (mimic)、miR-506 inhibitor(Inhi)、miR-506 mimic negative control(NC)和空白对照组(Blank)分别进行转染,通过MTT实验、Transwell实验和流式细胞术检测miR-506对绒毛膜细胞作用;通过双荧光素酶报告实验、qRT-PCR和Western blot检测miR-506、caveolin-1及相关蛋白的表达变化。[结果]与正常早孕绒毛相比,miR-506在绒毛膜癌组中表达显著降低(P<0.05),并与caveolin-1表达呈负相关。Mi R-506过表达明显抑制绒毛膜癌细胞的增殖、侵袭并促进凋亡。双荧光素酶报告实验表明miR-506过表达显著抑制野生型荧光素酶活性,qRT-PCR和Western blot结果显示miR-506过表达显著抑制caveolin-1表达,进而抑制相关蛋白表达。[结论] MiR-506可能通过靶向caveolin-1抑制绒毛膜癌细胞的增殖和侵袭。
[Purpose] To investigate the effect of microRNA-506 (miR-506) on proliferation and invasion ability of choriocarcinoma and its mechanisms.[Methods] The expression of miR-506 and caveolin-1 (CAV-1) in choriocarcinoma and normal early pregnancy villi was measured by qRTPCR. Human choriocarcinoma JEG3 JAR cells were transfected with miR-506 mimic,miR-506 inhibitor or miR-506 mimic negative control (NC),respectively. The cell proliferation,invasive ability and apoptosis were detected by MTT,transwell,and flow cytometry,respectively. The interaction,variation of miR-506 and CAV-1,the expressions of related proteins were detected by dual luciferase report assay,q RT-PCR and Western blot methods.[Results] Compared with normal early pregnancy villi,expression of miR-506 in choriocarcinoma significantly decreased (P<0.01) and it was negatively correlated with the expression of CAV-1. Dual luciferase report assay showed that miR-506 significantly inhibited activity of wild type luciferase in JEG3 and JAR cells. Overexpression of miR-506 significantly inhibited proliferation,invasion of choriocarcinoma cells and induced the cell apoptosis,and also significantly inhibited expression of CAV-1 and related proteins.[Conclusion] Mi R-506 may inhibit proliferation and invasion of choriocarcinoma cells via targeting CAV-1.
Bulletin of Chinese Cancer