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大蒜秸秆可溶性膳食纤维的超声复合酶解提取及其抗氧化性分析 预览

Extraction of soluble dietary fiber from garlic straw via ultrasonic-assisted enzymatic hydrolysis and evaluation of antioxidant activity
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摘要 为了增强大蒜秸秆膳食纤维的生理功能,研究了不同提取方法对其可溶性膳食纤维(Soluble dietary fiber,SDF)含量的影响.以SDF含量为响应值,对比分析超声、酶解及超声复合酶解法的改性效果,研究固液比、超声时间、超声功率、加酶量和酶解时间等因素对SDF得率的影响,通过羟基(·OH)和1,1-二苯基-2-苦基肼自由基(DPPH·)清除率评价SDF的体外抗氧化能力.结果表明,超声复合酶解法提取的SDF得率最高,通过单因素优化试验确定其最佳提取条件为固液比1∶30、超声功率300W、超声时间15min、加酶量5%、酶解时间4h;SDF对·OH和DPPH·的清除率较强,其IC50(50%Inhibitory concentration,IC50)分别为1.41和0.64mg/mL,且在一定浓度范围内,自由基清除率与SDF浓度呈现剂量效应线性关系,说明大蒜秸秆SDF是一种较好的抗氧化剂. In order to enhance the physiological function of garlic straw dietary fiber,the different extraction methods on the soluble dietary fiber(Soluble dietary fiber,SDF)content were studied.Taking SDF content as the response value,the modification effects of ultrasound,enzymatic hydrolysis and ultrasonic complex enzymatic hydrolysis were compared and analyzed,and the effects of solid-liquid ratio,ultrasound time,ultrasound power,enzyme amount and enzymatic hydrolysis time on the yield of SDF were studied.The antioxidant capacity of SDF in vitro was evaluated by the clearance rate of hydroxyl(·OH)and 1,1-diphenyl-2-amylhydrazine free radicals(DPPH·).The results suggested that when garlic straw was pretreated by ultrasonic assisted enzymatic hydrolysis method,the SDF yield was the highest.The optimal extraction conditions consisted of solid/liquid ratio of 1∶30,ultrasonic power of 300 W,ultrasonic time of 15 min,E/S ration of 5%and enzymatic hydrolysis of 4 h.The IC50 of SDF on OH·and DPPH·were obtained to be 1.41 and 0.64 mg/mL,respectively.Moreover,the relationship between free radical scavenging rate and SDF concentration was linear,suggesting that SDF was a good antioxidant.
作者 赵匀淑 程静 卢子博 李璇 陈文文 黄六容 ZHAO Yun-shu;CHENG Jing;LU Zi-bo;LI Xuan;CHEN Wen-wen;HUANG Liu-rong(School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,Jiangsu,China)
出处 《湖北农业科学》 2019年第6期117-120,127共5页 Hubei Agricultural Sciences
基金 江苏省大学生实践创新训练计划项目(201710299033Z) 江苏省青年基金项目(BK20170539).
关键词 大蒜秸秆 可溶性膳食纤维 超声 酶解 garlic straw soluble dietary fiber ultrasound enzymatic hydrolysis
作者简介 赵匀淑(1995-),女,贵州遵义人,在读本科生;通信作者:黄六容(1979-),女,副教授,博士,主要从事农产品物理加工技术研究,(电话)13775554336(电子信箱)hlr88888@163.com.
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