[Objective] The content of perillaldehyde in stems,leaves,fruit calyx and seeds of four kinds of Perilla frutescens from Fanjin Mountain (FMPF) was determined by high performance liquid chromatography(HPLC).[Method ]Based on the single factor test results,taking methyl alcohol as the extraction solvent,solid-liquid ratio and extraction time were considered for the further optimized using response surface method (RSM) when perillaldehyde content(PAC)was used as response.And other ultrasonic conditions were fixed for extraction temperature 35 ℃,times of1,frequency 40 kHz,power 300 W.Perillaldehyde from four partsof P.frutescens were extracted under optimal conditions and determined by HPLC.[Result] The optimum conditions were obtained as follows:solid-liquid ratio1 ∶60,extraction time24 min.PAC in stems,leaves,fruit calyx and seeds of the same P.frutescens were significantly differences ( P <0.05),leaves>fruit calyx>stems>seeds.PAC in leaves or fruit calyx between 4 kinds of P.frutescens also had significant differences ( P < 0.05),and PAC in leaves of FMPF1(broad ovate,purplish on both sides leaves and itsmargin is shallow sawtooth)or FMPF4(ovate,purplish on both sides leaves and itsmargin is deep sawtooth)were 7.537 and 6.139 mg/g,respectively,and PAC of their fruit calyx is 3.712 and 3.887 mg/g,respectively.[Conclusion] The P.frutescens (FMPF1,FMPF4) from Fanjing Mountain is a good resource for extracting perillaldehyde.Simultaneously,it is suggested that there is a correlation between leaf morphology and PAC.
Journal of Anhui Agricultural Sciences
Response surface method