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杭白菊提取液对小鼠肝纤维化的保护作用及机制研究 预览

Protective effect and mechanism of Dendranthema morifolium on hepatic fibrosis in mice
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摘要 目的探讨杭白菊对肝纤维化的保护作用及作用机制。方法SPF级小鼠72只随机分成6组。灌胃用药:正常对照组和模型对照组为0.9%NaCl注射液,水飞蓟宾组为5.5mg/mL水飞蓟宾,杭白菊低、中、高浓度组分别为杭白菊提取液150、300、600mg/mL;腹腔注射用药:除正常对照组为0.9%NaCl注射液外,其余均为0.1%四氯化碳(CCl4)摘除眼球法采血检测小鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、透明质酸(HA)、白细胞介素-6(IL-6)、超氧化物歧化酶(SOD)、丙二醛(MDA),取肝检测肝指数,取肝右叶行病理检测。结果模型对照组的血清ALT、AST、HA、IL-6、MDA均明显高于正常对照组(P<0.01),而肝指数明显低于正常对照组(P<0.01),肝细胞结构模糊,肝索排列紊乱甚至消失,肝细胞变性坏死区域明显由结缔组织代之。水飞蓟宾组的血清ALT、AST、HA、IL-6、SOD、MDA与正常对照组比较差异无统计学意义(P>0.05),而明显低于模型对照组(P<0.01,P<0.05)。杭白菊低、中、高浓度组的血清ALT、AST、HA(除杭白菊高浓度组)和MDA明显高于正常对照组而明显低于模型对照组(P<0.01);杭白菊低、中、高浓度组血清IL-6与正常对照组和模型对照组比较差异均无统计学意义(P>0.05);杭白菊低浓度组的血清SOD明显高于模型对照组(P<0.01),杭白菊高浓度组的血清SOD明显高于模型对照组和正常对照组(P<0.01,P<0.05);杭白菊中、高浓度组的血清HA明显低于杭白菊低浓度组(P<0.01,P<0.05),杭白菊高浓度组的MDA明显低于杭白菊低、中浓度组(P<0.01,P<0.05)。模型对照组、水飞蓟宾组和杭白菊低、中、高浓度组的肝指数之间的两两比较差异均无统计学意义(P>0.05)但均明显低于正常对照组(P<0.05,P<0.01)。水飞蓟宾组和杭白菊低、中浓度组的肝细胞结构较模型对照组清晰,肝细胞变性坏死区域有较大的改善;正常对照组和杭白菊高浓度组的肝细胞形态� Objective To investigate the protective effect and mechanism of Dendranthema morifolium on hepatic fibrosis in mice.Methods Seventy-two SPF mice were randomly divided into 6 groups:the normal control group(0.9%NaCl injection),model control group(0.9%NaCl injection),silybin group(5.5 mg/mL silybin),low,medium and high concentration of Dendranthema moritolium(150,300,600 mg/mL Dendranthema moritolium extract)group,each group was intragastrically administered with the corresponding drugs or solvents;except for the normal control group which intraperitoneal injection of 0.9%NaCl,the other were intraperitoneal injection of 0.1%carbon tetrachloride(CCl 4);Both intragastrically administion and intraperitoneal injection amount was 10 mL/kg.The mice in each group were intragastrically administed once a day for a week,then intragastrically administion and intraperitoneal injection for 4 weeks alternately,and then intragastrically administed again once a day for a week.The ALT,AST,HA,IL-6,SOD and MDA were detected through blood sampling at 30th min after the last gavage.The liver index was measured and the pathological examination was performed on the right lobe of the liver.Results The serum ALT,AST,HA,IL-6 and MDA in the model control group were significantly higher than those in the normal control group(P<0.01),while the liver index was significantly lower than that in the normal control group(P<0.01),the structure of hepatocytes was blurred,the arrangement of hepatic cord was disordered or even disappeared,and the degeneration and necrosis area of hepatocytes was obviously replaced by connective tissue.There was no difference of the serum levels of ALT,AST,HA(except for high concentration of Dendranthema group),IL-6,MDA in silybin group than in the normal control group(P>0.05),but it was lower than that in the model control group(P<0.01,P<0.05),there was no significant difference in HA between high concentration group and normal control group(P>0.05).There was no statistical difference in serum IL-6 between low,middle a
作者 梁育汝 王彩冰 陈由阳 罗红叶 屠兴荣 唐敏 LIANG Yuru;WANG Caibing;CHEN Youyang;LUO Hongye;TU Xingrong;TANG Min(Clinical Medical College,Youjiang Medical College for Nationalities,Baise,Guangxi 533000,China;Applied Physiology Laboratory,Youjiang Medical College for Nationalities,Baise,Guangxi 533000,China)
出处 《重庆医学》 CAS 2019年第8期1297-1301,共5页 Chongqing medicine
基金 2017年国家级大学生创新创业训练计划项目(201710599002)。
关键词 杭白菊 肝硬化 血清学 MDA 肝指数 小鼠 Dendranthema morifolium liver cirrhosis serology MDA liver index mice
作者简介 梁育汝(1995-),本科,主要从事消化内科研究;通信作者:王彩冰,E-mail:wangcb4444@163.com。
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