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周期性压应力诱导髁突软骨细胞分泌促破骨因子的研究 预览

Effects of Periodic Compression on Expression of Pro-osteoclastic Factors by Mandibular Condylar Chondrocytes
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摘要 目的:研究周期性压应力对髁突软骨细胞促破骨因子表达的影响。方法:分离原代大鼠髁突软骨细胞,并与琼脂糖培养基混合,采用Flexcell细胞加力系统对软骨细胞/琼脂糖混合物进行压应力加载,分别对细胞施加15、30及45 kPa周期性压应力(频率为0.5 Hz),加力时间分别为2、6及12 h。对照组采用相同处理方式,但不施加压应力。加力结束后收集细胞,采用实时定量聚合酶链反应(real-time quantitative polymerase chain reaction,Real-time PCR)及Western blot检测软骨细胞促破骨因子表达情况。结果:15 kPa压力刺激软骨细胞6 h即可促进软骨细胞核因子-κB(nuclear factor kappa-B,NF-κB)配体受体激活剂(the receptor activator of NF-κB ligand,RANKL)的表达(P<0.05),30及45 kPa压力刺激软骨细胞6及12 h可促进软骨细胞基质衍生因子1(stroma derived factor 1,SDF-1)、转化生长因子-β1(transforming growth factor-β1,TGF-β1)及RANKL的表达(P<0.05),但其单核细胞趋化蛋白-1(monocyte chemotactic protein-1,MCP-1)、巨噬细胞集落刺激因子(macrophage-colony stimulating factor,M-CSF)及骨保护素(osteoprotegerin,OPG)的表达则与对照组比较差异无统计学意义(P>0.05)。结论:周期性压应力可促进软骨细胞表达SDF-1、TGF-β1及RNAKL等促破骨因子。 Objective:To investigate the effects of periodic compression on the expression of pro-osteoclastic factors by mandibular condylar chondrocytes(MCCs).Methods:The rat MCCs were isolated and mixed with agarose culture medium.The Flexcell system was used to conduct compression on the MCCs/agarose mixture.The intensities of compression were 15,30,and 45 kPa(frequency 0.5 Hz),and the stimulating time were 2,6,and 12 hours.The control group was treated in the same way but without compression.Cells were collected after the application of force and the expression of pro-osteoclastic factors by MCCs were detected.Results:When the MCCs were stimulated by 15 kPa compression for 6 hours,the expression of RANKL was significantly higher than those of the controls.When the MCCs were stimulated by 30 and 45 kPa compression for 6 and 12 hours,the expression of SDF-1,TGF-beta-1,and RANKL were higher than those of the controls,but the gene expression of MCP-1,M-CSF,and OPG were not different from those of the controls.Conclusion:Periodic compression could promote the expression of pro-osteoclastic factors such as SDF-1,TGF-beta 1,and RANKL.
作者 阮国宪 高丽卿 邓立 RUAN Guo-xian;GAO Li-qing;DENG Li(Department of Prosthodontics,Stomatology Hospital of Hanzhong,Hanzhong 723000,China)
出处 《口腔医学研究》 CAS 北大核心 2019年第4期372-376,共5页 Journal of Oral Science Research
基金 汉中市口腔医院创新项目(编号:2015031).
关键词 颞下颌关节 髁突软骨细胞 压应力 破骨细胞 促破骨因子 Temporomandibular joint Mandibular condylar chondrocytes Compression Osteoclast Pro-osteoclastic factors
作者简介 通讯作者:阮国宪(1975-),男,浙江上虞人,本科,主治医师,主要从事颞下颌关节生物力学研究。E-mail:15029362225@163.com.
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  • 1Wang XD, Kou XX, He I)Q, et al. Progression of cartilage degradation, bone resorption and pain in rat temporomandib ular joint osteoarthritis induced by injection of iodocetate [J]. I'I.oS()ne,2012,7(9) e45036. 被引量:1
  • 2Schuiki I, Zhang I., Volchuk A. Endoplasmic Retieulum Redox Slate Is Not Perturbed by Pharmacological or Patho- logical Endoplasmie Reticulum Stress in IAve Pancreatic beta ells [J]. PI.oSOne02012.7(ll) : e48626. 被引量:1
  • 3Lakshmanan A P, Thandavarayan R A, Palaniyandi S S, et al. Modulation of AT 1R/CHOP-JNK-Castasel2 path- way by olmesartan treatment attenuates ER stress induced renal apoptosis in streptozotocin-induced diabetic mice [J]. Eur J Pharm Sci,2011,44(5) : 627 634. 被引量:1
  • 4Wang MQ, Xue F, He JJ, et al. Missing posterior teeth and risk of temporomandibular disorders [J]. J Dent Res,2009, 88(10) : 942-945. 被引量:1
  • 5Wang GW, Wang MQ, Wang XJ, et al. Changes in the ex- pression of MMP- 3, MMP- 9, TIMP- 1 and aggrecan in the condylar cartilage of rats induced by experimentally crea- ted disordered occlusion [J]. Arch Oral Biol,2010,55(11) : 887-895. 被引量:1
  • 6Jiao K, Wang MQ, Niu LN, et aL Mandibular condylar car- tilage response to moving 2 molars in rats [J]. Am J Orthod Dentofacial Orthop,2010,137(4) 460-461, 460-461. 被引量:1
  • 7Fu S, Watkins SM, Hotamisligil GS. The role of endoplas- mic reticulum in hepatic lipid homeostasis and stress signaling [J]. CellMetab,2012,15(5) : 623-634. 被引量:1
  • 8Xu K, Zhu XP. Endoplasmic reticulum stress and prion dis- eases[J]. RevNeurosci,2012,23(1) : 79-84. 被引量:1
  • 9Yang L, Meburney D, Tang SC, et al. A novel role for Bcl-.2 associated- athanogene- 1 (Bag- 1 ) in regulation of the endoplasmic reticulum stress response in mammalian chon- drocytes [J]. J Cell Biochem,2007,102(3) : 786-800. 被引量:1
  • 10Yang L, Carlson S G, Mcburney D, et al. Multiple signals induce endoplasmic retieulum stress in both primary and im- mortalized chondrocytes resulting in loss of differentiation, impaired cell growth, and apoptosis [J]. J Biol Chem,2005, 280(35) : 31156-31165. 被引量:1

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