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早孕因子单克隆抗体对黑色素瘤细胞A-375增殖、凋亡的影响

Effect of Monoclonal Antibody against Early Pregnancy Factor on Proliferation and Apoptosis of Melanoma cell A-375
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摘要 目的:研究早孕因子单克隆抗体(EPF-McAb)对黑色素瘤细胞A-375增殖、凋亡的影响。方法:体外培养黑色素瘤细胞A-375,通过CCK-8实验检测EPF-McAb对A-375细胞增殖的影响;通过流式细胞术检测EPF-McAb对A-375细胞凋亡的影响;Western Blot检测EPF-McAb对A-375细胞EPF蛋白表达的影响。结果:CCK-8结果显示EPF-McAb可以抑制黑色素瘤细胞A-375的增殖,且随着作用时间延长和药物浓度的增加,对A-375细胞增殖的抑制作用也增强;流式细胞术实验结果显示EPF-McAb可以促进黑色素瘤细胞A-375的凋亡,且调亡率随着药物浓度的增加而升高,0.2、0.4、0.8 mg/m L的EPF单抗作用于A-375细胞24 h后,细胞调亡率分别为:14.68%(P<0.01)、19.81%(P<0.01)、23.97%(P<0.01);Western Blot实验结果显示EPF-McAb可以降低黑色素瘤细胞A-375 EPF蛋白的表达。结论:早孕因子单克隆抗体可以抑制黑色素瘤细胞A-375的增殖,并促进其凋亡。 Objective:To study the effect of early pregnancy factor monoclonal antibody(EPF-McAb)on proliferation and apoptosis of melanoma cell A-375.Methods:CCK-8 experiment,flow cytometry and Western Blot were conducted on cultured melanoma cells A-375 in vitro to understand the effect of EPF-McAb on proliferation,apoptosis and protein expression of A-375 cells respectively.Results:CCK-8 results showed that EPF-McAb could inhibit the proliferation of A-375 in melanoma cells,and the effect was also enhanced with the prolongation of action time and the increase of drug concentration.The results of flow cytometry showed that EPF-McAb could promote the apoptosis of A-375 in melanoma cells,and the rate of apoptosis was associated with the drug concentration.The increase of 0.2,0.4,0.8 mg/m L EPF monoclonal antibody on A-375 cells 24 h,the cell apoptosis rate was 14.68%(P<0.01),19.81%(P<0.01),23.97%(P<0.01);Western Blot experimental results showed that EPF-McAb can reduce the expression of A-375 EPF protein in melanoma cells.Conclusion:EPF-McAb can not only inhibit the proliferation of melanoma cell A-375,but also promote its apoptosis.
作者 吴文林 钟永怡 周泉 唐杰 王家骥 WU Wen-lin;ZHONG Yong-yi;ZHOU Qum;TANG Jie;WANG Jia-ji(School of Public Health,Guangzhou Medical University,Guangzhou,Guangdong,511436,China)
出处 《现代生物医学进展》 CAS 2019年第5期820-823,共4页 Progress in Modern Biomedicine
基金 广东省自然科学基金项目(2015A030313455).
关键词 早孕因子 单克隆抗体 黑色素瘤 Early pregnancy factor Monoclonal antibody Melanoma
作者简介 吴文林(1992-),硕士研究生,主要研究方向挪瘤免疫学,E-mail:wuwenlin2020@163.com;通讯作者:王家骥(1955-),硕士生导师,教授,主要研究方向:全科医学,E-mail:wjiaji@163.com.
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