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枯草芽孢杆菌BC80-6发酵条件的优化及对烟草根黑腐病的控病效果 预览 被引量:2

Optimization of fermentation conditions for Bacillus subtilis BC80-6 and its control on Thielaviopsis basicola of tobacco
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摘要 探究了3种枯草芽孢杆菌BC80-6处理液对烟草根黑腐病菌菌丝生长、孢子萌发的抑制作用,对其发酵条件进行了优化,并开展田间控病防效试验。结果表明:10-1倍枯草芽孢杆菌BC80-6的活菌液对烟草根黑腐病菌菌丝生长和孢子萌发的抑制效果最好,抑制率分别为93.2%、95.3%。通过单因素试验及正交试验对菌株BC80-6培养基的配方进行优化,确定培养基的最佳配方为蔗糖1.5%、酵母浸出物0.5%、硫酸镁0.06%(质量体积比)。最佳发酵条件:发酵温度28℃、时间24h、初始pH6.5、摇床转速180r/min、接种量5%、装液量40mL(250mL三角瓶)。枯草芽孢杆菌活菌液对烟草根黑腐病的田间防效为88.19%。 To clarify the effects of Bacillus subtilis BC80-6 on Thielaviopsis basicola,the inhibitory effects of three treatment solutions of B.subtilis BC80-6 on the mycelial growth and spore germination of T.basicola were investigated.BC80-6 fermentation conditions were optimized and field control experiments were carried out.The results showed that 10-1 times living bacteria solution of B.subtilis BC80-6 had the best inhibitory effects on the mycelial growth and spore germination of B.subtilis with the inhibition rates of 93.2%and 95.3%,respectively.Via a single factor test and an orthogonal experiment,the optimum BC80-6 medium was 1.5%sucrose,0.5%yeast extract and 0.06%magnesium sulfate(mass/volume ratio).The best fermentation condition was fermentation temperature 28℃,time 24 h,initial pH 6.5,rotation speed 180 r/min,inoculation amount 5%(v/v)and medium volume 40 mL in 250 mL triangular bottle.The field control effect of living bacteria solution of B.subtilis on tobacco root black rot was 88.19%.
作者 王文凤 张丽娜 朱启法 汪文杰 周本国 檀根甲 许大凤 WANG Wenfeng;ZHANG Lina;ZHU Qifa;WANG Wenjie;ZHOU Benguo;TAN Genjia;XU Dafeng(Anhui Agricultural University,Hefei 230036,China;Anhui Provincial Tobacco Company of CNTC,Hefei 230071,China;Anhui Wannan Tobacco Co.,Ltd.,Xuancheng 242000,Anhui,China;Chizhou Branch of Anhui Provincial Tobacco Company,Chizhou 247000,Anhui,China;Institute of Tobacco,Anhui Academy of Agricultural Sciences,Hefei 230031,China)
出处 《烟草科技》 EI CAS CSCD 北大核心 2019年第5期6-13,共8页 Tobacco Science & Technology
基金 安徽省烟草公司科技项目“烟草根黑腐病流行灾变规律及绿色防控技术研究”(20150551006)。
关键词 烟草 根黑腐病菌 枯草芽孢杆菌 发酵条件 田间防效 Tobacco Thielaviopsis basicola Bacillus subtilis Fermentation condition Field control effect
作者简介 王文凤(1993—),在读硕士研究生,研究方向:植物病理学。E-mail:1277389397@qq.com;通信作者:檀根甲,E-mail:tgj63@163.com;许大凤,E-mail:dafengxu0918@sina.com.
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