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基于超高效液相色谱-四极杆-飞行时间质谱技术的黄芪注射液中化学成分分析 预览

Comprehensive Screening of Multi-components in Huangqi Injection by Ultra-performance Liquid Chromatography Coupled with Quadrupole-time-of-flight Mass Spectrometry
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摘要 目的:基于超高效液相色谱-四级杆-飞行时间质谱(Ultra-performance Liquid Chromatography Quadrupole Time-of-flight Mass Spectrometry,UPLC/Q-TOF-MS)对黄芪注射液物质基础进行了全面的研究,建立及黄芪注射液中有关成分的快速准确的分析方法。方法:采用Agilent Eclipse Plus C 18 色谱柱(3 mm×100 mm,1.8 μm),以含0.1%甲酸水溶液-乙腈为流动相,梯度洗脱,流速0.4 mL/min;电喷雾源(ESI),Q-TOF-MS作检测器,正、负离子模式检测;甲醇稀释样品;与对照品保留时间、母离子及碎片离子精确分子量,比对准确定性。结果:共鉴定黄芪注射液中的46个化合物,包括氨基酸,黄酮,皂苷,核苷和烟酸等。结论:建立了黄芪注射液中的复杂化学成分的快速检测和评价方法,为其的质量控制及工艺改进提供参考。 Objective: To study comprehensive screening multi-components in Radix Astragali and Huangqi injection,based on high-resolution quadrupole-time-of-flight mass spectrometry. Methods: The analysis was performed by Agilent Eclipse Plus C 18 column(3 mm × 100 mm,1.8 μm)with the mixture of 0.1% formic acid aqueous solution-acetonitrile as mobile phase by gradient elution,and the flow rate was 0.4 mL/min.Q-TOF-MS equipped with ESI ion source was performed in both positive and negative ionization.Samples were diluted with methanol.Qualitative analyze was based on retention time,the accurate mass of the precursor ion and product ions. Results: A total of 46 components including amino acids,flavonoids,saponins,nucleosides and nicotinic acid were successfully identified or tentatively characterized. Conclusion: This study is expected to provide an effective pattern for systematic characterization of the complex Huangqi injection systems,and establish a reliable strategy for its quality evaluation,also offers a reference for quality control as well as technological improvement.
作者 于泓 胡青 孙健 冯睿 张甦 张静娴 毛秀红 季申 Yu Hong;Hu Qing;Sun Jian;Feng Rui;Zhang Su;Zhang Jingxian;Mao Xiuhong;Ji Shen(Shanghai Institute for Food and Drug Control,Shanghai 201203,China)
出处 《世界中医药》 CAS 2019年第4期809-817,共9页
基金 上海市科学技术委员会基金项目(18DZ2292200,12DZ1970402).
关键词 黄芪注射液 黄芪 化学成分 Q-TOF-MS 皂苷 黄酮 氨基酸 核苷 Huangqi injection Radix Astragali Components Quadrupole-time-of-flight mass spectrometry Saponins Flavonoids Amino acids Nucleosides
作者简介 于泓(1987.09—),女,硕士,主管药师,研究方向:药物分析,E-mail:yuhong-918@163.com;通信作者:季申(1963.08-),女,主任药师,研究方向:中药质量控制和安全性检测技术研究,E-mail:jishen2013@163.com.
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