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超敏HBV DNA检测在慢性HBV感染者中的动态监测价值 预览

Value of hypersensitive HBV DNA detectionin dynamic monitoring of chronic HBV infection
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摘要 目的探讨超敏检测技术对传统PCR法检测HBV DNA阴性的慢性乙型肝炎病毒(HBV)感染者的临床应用价值。方法收集370例传统PCR法检测HBV DNA≤500 IU/ml的慢性HBV感染者的血清标本,同时检测对应的超敏 HBV DNA水平,并分析与谷丙转氨酶(ALT)、乙型肝炎病毒E抗原(HbeAg)以及肝功能其他指标的相关性。结果超敏检测法(-)和超敏检测法(+)两组中ALT异常率比较差异有统计学意义(χ^2=3.905, P =0.048),HbeAg阳性率比较差异有统计学意义(χ^2=14.264, P <0.05);按抗病毒治疗时间不同,超敏HBV DNA阳性率趋势检验结果显示负相关(χ^2=39.823, P ><0.001),而ALT异常中超敏HBV DNA阳性率比较差异有统计学意义(χ^2=18.922, P =0.001),其中两两比较采用Bonferroni法调整α水平,显示未治疗组与治疗两年、三年差异有统计学意义( P =0.002, P =0.001)。结论超敏定量检测技术对传统法检测HBV DNA阴性的患者,可以更加准确了解病毒复制情况,帮助选择最佳治疗方案,预测预后。 Objective To explore the clinical application value of hypersensitivity detection technology in patients with chronic hepatitis B virus(HBV) infection with HBV DNA negative by traditional PCR assay. Methods The serum samples of 370 patients with chronic HBV infection with HBV DNA≤500 IU/ml were collected by traditional PCR assay.Meanwhile,the corresponding hypersensitive HBV DNA level were detected,and the correlation with alanine aminotransferase(ALT),hepatitis B virus E antigen(HbeAg) and other indicators of liver function were analyzed. Results Hypersensitive detection assay(-) and hypersensitive detection assay(+) showed a statistically significant difference in the ALT anomaly rate between the two groups(χ^2=3.905, P =0.048),and the difference in positive rate of HBeAg was statistically significant(χ^2=14.264, P <0.05).According to the different antiviral treatment time,the trend test results of hypersensitive HBV DNA positive rate showed a negative correlation(χ^2=39.823,P <0.001),and the difference of hypersensitive HBV DNA positive rate in ALT abnormality was statistically significant(χ^2=18.922, P =0.001). Bonferroni method was used to pairwise comparison and adjusted the level of alpha. The results showed that there was significant difference between untreated group and the treated group for two years( P =0.002) and three years( P =0.001). Conclusion Hypersensitive quantitative detection technology can provide a more accurate understanding of virus replication in patients with HBV DNA negative by traditional assay,and contribute to select the best treatment plan and predict the prognosis.
作者 王同同 郜玉峰 曹雯君 叶郡 邹桂舟 李绪桐 孔雪杰 Wang Tongtong;Gao Yufeng;Cao Wenjun(Dept of Infectious Disease,The First Affiliated Hospital of Anhui Medical University,Hefei 230022;Dept of Infectious Disease,The Second Affiliated Hospital of Anhui Medical University,Hefei 230601)
出处 《安徽医科大学学报》 CAS 北大核心 2019年第5期785-788,共4页 Acta Universitis Medicinalis Anhui
基金 安徽省自然科学基金(编号:1608085MH164).
关键词 乙型肝炎病毒 超敏检测 传统PCR法 谷丙转氨酶 hepatitis B virus hypersensitivity detection traditional PCR assay alanine transaminase
作者简介 王同同,女,硕士研究生;责任作者:郜玉峰,男,主任医师,副教授,硕士生导师,E-mail: aygyf@126.com.
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