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响应面法优化斑花黄堇中总黄酮的提取工艺

Optimization of Extraction Process of Total Flavonoids from Corydalis conspersa Maxim.by Response Surface Methodology
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摘要 目的采用Box-Benhnken设计并结合响应面分析法对斑花黄堇总黄酮的提取工艺进行优化。方法以芦丁标准品溶液为对照,采用紫外分光光度法测定总黄酮含量;以60%乙醇为溶剂,考察液料比、提取时间和提取温度对斑花黄堇总黄酮提取得率的影响。结果优化后斑花黄堇总黄酮的提取工艺为:液料比为500mL·g^-1,提取温度为70℃,提取时间为60min。应用优化后的提取工艺总黄酮的得率为2.94%,与理论值2.99%的相对标准偏差为0.27%。结论Box-Benhnken响应面优化斑花黄堇中总黄酮的提取工艺稳定、可行。 Objective The research adopts the method of the Box-Benhnken design combining with response surface analysis to optimize the extraction technology of total flavonoids from Corydalis conspersa. Methods Content of total flavonoids was determined using rutin as the reference and UV spectrometry. Using 60% ethanol as solvent, the influence of ratio of liquid-solid,extraction time and extraction temperature on the extraction yield of flavonoids was evaluated. Results Optimum extraction technology of total flavonoids yield was as follows:ratio of liquid-solid is 500 mL·g-1,extraction temperature is 70 ℃,extracting time is 60 min. Under the optimized extraction conditions,the extraction rate of the total flavonoids was 2.94% and the relative standard odeviation between the predicted(2.99 %)and measured value was 0.27%. Conclusion It is a feasible and stable method to obtain the total flavonoids from the Corydalis conspersa using the ultrasonic optimization extraction technology process combined with Box-Benhnken design and response surface analysis.
作者 杜清 吴江 刘明地 候永强 郭苏城 朋毛措 才让卓玛 任卓 DU Qing;WU Jiang;LIU Mingdi;HOU Yongqiang;GUO Suchcng;PENG Maocuo;CAIRANG Zhuoma;REN Zhuo(College of Pharmacy,Qinghai Nationalities University,Key Laboratory of Plant Resources of Qinghai-Tibet Plateau in Chemical Research,Xining 810007 Qinghai,China;College of Chemistry and Chemical Engineering,Xining 810007 Qinghai,China;College of Ecological Environment and Resource,Qinghai Nationalities University,Xining 810007 Qinghai,China)
出处 《中药新药与临床药理》 CAS CSCD 北大核心 2019年第5期608-613,共6页 Traditional Chinese Drug Research & Clinical Pharmacology
基金 青海省科技厅项目(2016-ZJ-903) 青海省科技厅重点实验室发展专项(2017-ZJ-Y19).
关键词 斑花黄堇 响应面分析法 总黄酮 Box-Benhnken设计 提取工艺 样品测定 Corydalis conspersa Maxim. response surface analysis total flavonoids Box- Benhnken design extracting technology sample determination
作者简介 杜清,女,博士,中级工程师,从事中药学教学与植物分子质量控制、疾病研究工作。Email:bjdfydq@163.com.
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