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四妙勇安汤活性部位配伍方薄层色谱鉴别及绿原酸和哈巴俄苷的含量测定 预览

TLC Identification and Determination of Chlorogenic Acid and Habaroside in Active Part of Simiao Yongan Decoction
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摘要 目的:构建四妙勇安汤活性部位配伍方薄层色谱鉴别方法和HPLC测定绿原酸和哈巴俄苷的含量测定方法。方法:采用薄层色谱法对配伍方中玄参、甘草进行鉴别,采用HPLC对配伍方中绿原酸和哈巴俄苷含量进行检测。结果:薄层色谱鉴别中配伍方中在与对照品哈巴俄苷、甘草酸铵相应位置上均显相同颜色的荧光斑点,斑点清晰,阴性对照无干扰。绿原酸和哈巴俄苷分别在0.195~1.560(r=0.9999)、0.0192~0.3840μg·μL^-1(r=1.0000)线性关系良好,平均加样回收率分别为96.5%、97.8%,RSD分别为0.9%、1.1%(n=9)。结论:薄层鉴别及绿原酸和哈巴俄苷的含量测定方法专属性强,准确稳定、重复性好,能够有效控制该配伍方的质量。 Objective:To establish a TLC method for identification of active part and an HPLC method for determination of chlorogenic acid and habaroside in Si-Miao-Yong-An decoction by TLC.Methods:TLC was used to identify Radix Scrophulariae and Radix Glycyrrhizae,and HPLC was used to detect the content of chlorogenic acid and habaroside in decoction.Results:The fluorescence spots with decoction in the corresponding positions of Habaroside and ammonium glycyrrhizate were identified in TLC,and the spots were clear and the negative controls were not disturbed.Chlorogenic acid and habaroside had good linear relationship in the range of 0.195-1.560μg·μL^-1(r=0.999 9)and 0.019 2-0.384 0μg·μL^-1(r=1.000 0)respectively,and the average recovery rate was 96.5%(n=9)and 97.8%(n=9),RSD was 0.9%and 1.1%,respectively.Conclusion:The TLC identification and determination of chlorogenic acid and habaroside are strong in specificity,accurate,stable and reproducible,and can effectively control the quality of the active part of Si Miao Yong An decoction.
作者 杨会 宋珂 李慧 白云绮 王淑琪 吴圣贤 陈立新 聂波 YANG Hui;SONG Ke;LI Hui;BAI Yun-qi;WANG Shu-qi;WU Sheng-xian;CHEN Li-xin;NIE Bo(School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China;Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China)
出处 《中国现代中药》 CAS 2019年第5期668-672,共5页 Modern Chinese Medicine
基金 国家科技重大专项课题(2017ZX09304019,2009ZX09103-318).
关键词 四妙勇安汤 绿原酸 哈巴俄苷 薄层鉴别 高效液相色谱 Simiao Yongan decoction Chlorogenic acid Habaroside TLC identification HPLC
作者简介 通信作者:聂波,研究员,研究方向:中药活性成分体内外代谢研究;Tel:(010)84013190,E-mail:nieboww_1977@163.com.
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