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黄皮叶绿体基因组测序与分析 预览

Sequencing and Analysis of Chloroplast Genome of Clausena lansium (Lour.)Skeels
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摘要 [目的]分析黄皮[Clausena lansium(Lour.)Skeels)]叶绿体基因组,为黄皮属不同种或品种的进化、杂交、演变,以及黄皮不同品种的鉴定等提供技术支持。[方法]使用试剂盒提取了黄皮的叶绿体DNA,通过测序、组装、注释获得了黄皮叶绿体基因组。[结果]黄皮叶绿体基因组全长159283bp,其中反相重复序列区(IRs)长53998bp,大单拷贝序列区(LSC)和小单拷贝序列区(SSC)长度分别为87301、17983bp,共注释126个基因,包括编码蛋白基因89个,tRNA基因29个和rRNA基因8个。黄皮叶绿体基因组全序列GC含量38.7%。对5种芸香科果树的全长序列、SSC、LSC、IRA、IRB进行比较分析发现,假黄皮的叶绿体全长序列、LSC、IRA、IRB区域的长度较其他4种果树长,柠檬的SSC区域长度最长,黄皮的全长序列和SSC区域长度最短。黄皮叶绿体的基因数目和编码蛋白数目较其他4种果树多。对20种园艺植物的叶绿体基因组进行分析发现,同一科下面的不同属植物或同一属下面的不同种更容易聚为一类。[结论]该研究丰富了热带果树的叶绿体基因组数据库,为种质资源的合理开发利用提供了依据。 [Objective] The analysis of the chloroplast genome of Clausena lansium (Lour.)Skeels provides technical support for the evolution,hybridization and evolution of different species or varieties of Clausena lansium ,as well as the identification of different varieties of Clausena lansium .[Method] The chloroplast DNA was extracted from Clausena lansium by kit,and chloroplast genome was obtained by sequencing,assembly and annotation.[Result] The chloroplast genome is 159 283 bp in length,of which the inverted repeats sequence (IRs) is 53 998 bp,the large single-copy region(LSC) and the small single copy region (SSC) are 87 301 and 17 983 bp,respectively. A total of 126 genes are annotated,including 89 coding protein genes,29 tRNA genes and 8 rRNA genes.The full-sequence GC content of the chloroplast genome was 38.7%. The full-length sequences,SSC,LSC,IRA and IRB of five Rutaceae fruit trees,were compared and analyzed. It was found that the chloroplast full-length sequences,LSC,IRA and IRB regions of Clausena excavata were longer than those of the other four fruit trees,and the Citrus limon in SSC region was the longest and the Clausena lansium was the shortest.And the whole length sequence of Clausena lansium is also the shortest among the five species.The number of genes and coding proteins of chloroplasts in Clausena lansium was more than that in other four fruit trees. Analysis of the chloroplast genomes of 20 horticultural plants revealed that different genus plants under the same family or different species under the same genus were more likely to be clustered into one group.[Conclusion]This study enriched the chloroplast genome database of tropical fruit trees and provided a basis for the rational development and utilization of germplasm resources.
作者 赵志常 高爱平 黄建峰 罗睿雄 ZHAO Zhi-chang;GAO Ai-ping;HUANG Jian-feng(Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Haikou,Hainan 571101;National Cultivar Improvement Center of Tropical Fruit Tree,Haikou,Hainan 571101)
出处 《安徽农业科学》 CAS 2019年第11期115-118,共4页 Journal of Anhui Agricultural Sciences
基金 国家自然科学基金资助项目(31471850) 国家重点研发计划(2018YFD1000500) 非营利性科研机构改革启动经费(1630032017005 1630032017004)。
关键词 黄皮 叶绿体基因组 测序与分析 Clausena lansium (Lour.)Skeels Chloroplast genome Sequencing and analysis
作者简介 赵志常(1977—),男,山东烟台人,副研究员,从事热带果树遗传育种与分子生物学研究;通信作者:高爱平,研究员,从事果树资源学方面研究。
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