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兔VX2乳腺癌淋巴转移模型的建立及超声对其诊断的价值 预览

THE ESTABLISHMENT OF A RABBIT LYMPH NODE METASTASIS MODEL OF VX2 BREAST CANCER AND THE VALUE OF ULTRASOUND IN ITS DIAGNOSIS
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摘要 目的本研究用VX2乳腺癌组织块种植法建立新型乳腺癌腋窝淋巴结转移模型,探讨超声在实验兔VX2乳腺癌腋窝转移性淋巴结定性诊断中的应用价值。方法 采用组织块种植法将VX2传代瘤株接种于22只雌性新西兰种大白兔右侧第3乳头的乳垫内,原位肿瘤生成后触诊检查腋窝淋巴结有无肿大,待触及肿大的淋巴结时切除原位乳腺癌组织并行病理组织学检查。第5周对实验兔淋巴结行超声检查,依据其诊断标准评价淋巴结的性质,所得结果与病理组织学检查结果进行比较。结果 第2周时,20只实验兔可触及乳腺内的质韧结节,超声显示类圆形、边界尚清,内部回声尚均匀的低回声实性结节,肿瘤内部及周边血流丰富。3周后,触诊发现18只实验兔有肿大淋巴结发生。第5周超声检查示转移性淋巴结35枚,非转移性淋巴结7枚。以病理组织学结果作为金标准,基于淋巴结个数,超声诊断转移性淋巴结的准确性为85.7%(36/42),灵敏度为89.2%(33/37),特异度为 60.0%(3/5),建模成功率为81.8%(18/22)。超声与病理组织学两种方法对转移性淋巴结的诊断差异有统计学意义(χ 2 =4.541, P <0.05)。结论 组织块种植法建立的新型大白兔乳腺癌淋巴转移模型创伤小,成功率高,易发生淋巴结转移。常规超声对诊断兔乳腺癌转移性淋巴结有一定的应用价值。 Objective To establish a new model of breast cancer axillary lymph node metastasis by VX2 breast cancer tissue implantation, and to investigate the value of ultrasound in the qualitative diagnosis of axillary metastatic lymph nodes in rabbits with VX2 breast cancer. Methods The VX2 subcultured tumor strain was inoculated into the breast pad of the right third nipple of 22 female New Zealand white rabbits by tissue implanation. After in situ tumor formation, palpation was performed to check whether the axillary lymph nodes were enlarged. When the enlarged lymph nodes were touched, the orthotopic breast cancer tissues were resected for histopathological examination. At the 5th week, the experimental rabbits' lymph nodes were examined by ultrasonography, and the nature of the lymph nodes was evaluated according to the diagnostic criteria;the results obtained were compared with those of histopathological examination. Results At the 2nd week, tough nodules in the mammary gland were touched in 20 experimental rabbits, ultrasound showed a quasi-circular, clear boundary, low-echo solid nodules with uniform internal echo, and abundant blood flow inside and around the tumor. Three weeks later, palpation revealed that 18 rabbits had enlarged lymph nodes. At the 5th week, ultrasound examination revealed 35 metastatic lymph nodes and 7 non-metastatic lymph nodes. Using the histopathological results as the gold standard, based on the number of lymph nodes, the accuracy of ultrasound diagnosis of metastatic lymph nodes was 85.7%(36/42), the sensitivity was 89.2%(33/37), the specificity was 60.0%(3/5), and the modeling success rate was 81.8%(18/22). There were significant differences in the diagnosis of metastatic lymph nodes between ultrasound and histopathological (χ 2=4.541, P <0.05). Conclusion The new white rabbit model of breast cancer lymph node metastasis established by tissue implantation has small trauma and high success rate and is prone to lymph node metastasis. Conventional ultrasonography has certain value in the
作者 李文军 朱红 孙业全 朱晴 延春雨 刘峰 LI Wenjun;ZHU Hong;SUN Yequan;ZHU Qing;YAN Chunyu;LIU Feng(Department of Medical Imaging, Weifang Medical University, Weifang 261053, China)
出处 《精准医学杂志》 2019年第3期245-248,共4页 Journal of Precision Medicine
基金 国家自然科学基金资助项目(81373322).
关键词 乳腺肿瘤 淋巴转移 超声检查 病理学 乳房肿瘤 实验性 模型 动物 Breast neoplasms Lymphatic metastasis Ultrasonography Pathology Mammary neoplasms, experimental Models, animal Rabbits
作者简介 通讯作者:刘峰,Email:liufengwfmc@163.com.
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