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何首乌多糖的结构表征及其免疫调节活性研究

Structural characterization of polysaccharide from Polygonum multiflorum and its immunomodulatory activity
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摘要 目的从何首乌Polygonum multiflorum中提取分离多糖,对其结构进行表征,并评价其免疫调节活性。方法采用水提、醇沉法从何首乌中提取粗多糖,经Q-Sepharose Fast Flow强阴离子交换色谱柱分离纯化得到何首乌多糖(PMT)。利用高效凝胶渗透色谱-十八角度激光光散射联用法(HPGPC-MALLS)测定PMT的绝对分子质量,采用PMP柱前衍生-高效液相色谱法(HPLC)测定单糖组成。采用二维核磁共振波谱(2D-NMR)对PMT的结构进行表征。采用四甲偶氮唑盐(MTT)法、中性红比色法和Griess法分别检测PMT对小鼠单核巨噬细胞RAW264.7的生长、吞噬活性和释放一氧化氮(NO)能力的影响。结果 PMT是一种α-1,4-葡聚糖,其绝对分子质量为3.96×10^5。PMT可显著促进RAW264.7细胞的增殖,促进细胞的吞噬能力,增加NO的释放量。结论何首乌来源的α-1,4-葡聚糖具有显著的免疫调节活性,具有药物开发潜力。 Objective To extract and separate a polysaccharide from Polygonum multiflorum, characterize its structural features and study its immunomodulatory activity. Methods The polysaccharide from P. multiflorum(PMT) was isolated and purified by water extraction and ethanol precipitation following Q-Sepharose Fast Flow ion exchange chromatography column. Molecular weight of PMT was determined by high performance gel permeation chromatography-multiple angle laser light scattering(HPGPC-MALLS),and monosaccharide composition was analyzed by HPLC with PMP(1-phenyl-3-methyl-5-pyrazolone) pre-column derivatization,respectively. The structure of PMT was characterized by proton nuclear magnetic resonance spectrum(2 D-NMR). The immunomodulatory activities were tested by MTT, neutral red colorimetric assay and Griess method. Results PMT was a kind ofα-1,4-glucan, and its molecular mass was 3.96 × 10^5. PMT promoted the proliferation and phagocytosis of RAW 264.7 cells, and significantly induced the increase of NO production in a dose-dependent manner. Conclusion The polysaccharide from P.multiflorum is a linear α-1,4-glucan with potent immunomodulatory activity, which would be potentially developed as an effective drug.
作者 王娅 闫丽娜 孙甜甜 郝杰杰 李佳 胡明华 范罗嫡 蔡超 于广利 WANG Ya;YAN Li-na;SUN Tian-tian;HAO Jie-jie;LI Jia;HU Ming-hua;FAN Luo-di;CAI Chao;YU Guang-li(Key Laboratory of Marine Drugs,Ministry of Education,Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China;Inflnitus (China) Company Ltd.,Guangzhou 510600,China)
出处 《中草药》 CAS CSCD 北大核心 2019年第10期2290-2295,共6页 Chinese Traditional and Herbal Drugs
关键词 何首乌 何首乌多糖 α-1 4-葡聚糖 结构表征 免疫活性 Polygonum multiflorum Thunb. polysaccharide from Polygonum multiflorum α-1,4-glucan structural characterization immunomodulatory activity
作者简介 王娅(1993—),女,在读硕士,研究方向为糖化学与糖生物学。Tel:(0532)82031790,E-mail:yawangouc@163.com;通信作者:于广利(1964-),男,教授,主要从事糖化学与糖药物学研究。Tel:(0532)82031609,E-mail:glyu@ouc.edu.cn.
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