Objective: To analyze the distribution of clinical pathogenic bacteria of neonatal septicemia and their antimicrobial resistance as well as treatment strategies, and to provide a reference for the selection of drugs for initial empirical treatment. Methods: Clinical data of 81 neonatal septicemia patients admitted to the neonatal ICU from January 2015 to October 2018 were extracted, and blood bacterial culture and drug sensitivity results were analyzed. Results: 81 pathogenic bacteria were isolated from 81 cases of neonatal septicemia, among which 12 strains of Gram-negative bacteria accounted for 14.81% and 69 strains of Gram-positive bacteria accounted for 85.19%. The results of drug sensitivity test showed that the Gram-negative bacteria were resistant to ampicillin but highly sensitive to imipenem, amikacin and piperacilin-tazobactam. Gram-positive bacteria were mainly Staphylococcus epidermidis and Staphylococcus aureus, with a sensitivity of 100.00% to vancomycin and linezolid, and significant resistance to penicillin and erythromycin.Staphylococcus epidermidis accounted for 29.63% of 61 strains of delayed neonatal septicemia. Conclusion: The main pathogenic bacteria detected in delayed neonatal septicemia patients were Staphylococcus epidermidis and Staphylococcus aureus.