Objective:To investigate protective mechanism of gentiopicroside on articular cartilage of rats with traumatic osteoarthritis through nuclear factor κB(NF-κB) signaling pathway. Methods:30 male SD rats were divided into control group,model group and gentiopicroside group according to the random number table method. Rats in the model group and gentiopicroside group were used to establish the traumatic osteoarthritis model. While the rats in control group only had the articular cavity exposed. 1 wk after model establishment,the rats in the gentiopicroside group were given 50 mg/kg gentiopicroside solution by gavage,and the same amount of distilled water was given to the control and model groups once a day for 8 weeks. Thereafter,the Pathological examination and Mankin score were performed. The levels of nitric oxide(NO) and tumor necrosis factor-α(TNF-α) in articular cartilage tissues as well as the levels of NF-κB and inhibitor of NF-κB(I-κB) were detected. Results:The degeneration and necrosis degree were more mild in the gentiopicroside group than in the model group;The Mankin score as well as higher levels of NO,TNF-α,and NF-κB among three groups in a descending order was the model group,gentiopicroside group and control group,while the I-κB level among three groups in an ascending order was model group,gentiopicroside group and control group groups (P < 0.05). Conclusion:Gentiopicroside can ameliorate the morphology of articular cartilage in rats with traumatic osteoarthritis,decrease the content of NO and TNF-α in cartilage tissue,and reduce cartilage degeneration. The mechanism may relate to the decreasing level of NF-κB protein and increasing level of I-κB protein.
Journal of Emergency in Traditional Chinese Medicine