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诱变选育木聚糖酶高产菌及其发酵条件优化 预览

Breeding of Xylanase High-producing Strain by Mutation and Optimization of its Fermentation Conditions
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摘要 采用微波辐照对木聚糖酶产生菌疏棉状嗜热霉菌(Thermomyces lanuginosus)CICC2691进行诱变选育,考察不同微波功率和辐照时间对菌株致死率和正突变率的影响,得到最佳的诱变条件:微波辐照功率为500W,辐照时间为60s。在最佳诱变条件下得到一株高产木聚糖酶且能稳定遗传的菌株W5-133,其所产木聚糖酶酶活较出发菌株提高了66.8%。以菌株W5-133为研究对象,经单因素试验和响应面分析法对其发酵产酶条件进行了优化,得到最优发酵产酶条件:葡萄糖质量浓度为2.35g/L,发酵温度为56.0℃,发酵时间为99.5h,初始pH值为5.8,该条件下的木聚糖酶酶活为186.81U/mL,比优化前提高了24.4%。 Microwave irradiation mutagenesis was used to mutagenize xylanase-producing strain of Thermomyces lanuginosus CICC 2691. Optimum conditions of 500 W of the microwave irradiation power and 60 s of the irradiation time were investigated and obtained according to the lethality and positive mutation rate. Under optimal conditions, a strain named W5-133 with high xylanase capacity and hereditary stability was obtained. The ability of xylanase production increased by 66.8% compared to the original strain. Then, the fermentation conditions of W5-133 were optimized by single factor and response surface optimization methods. The optimal conditions were as follows: Glucose content 2.35 g/L, fermentation temperature 56.0℃, fermentation period 99.5 h, initial pH 5.8. Under these conditions, the xylanase activity of W5-133 achieved 186.81 U/mL, increased by 24.4% before optimization.
作者 朱慧霞 方桢 汪水玲 马晓静 姚日生 ZHU Hui-xia;FANG Zhen;WANG Shui-ling;MA Xiao-jing;YAO Ri-sheng(School of Food and Biological Engineering, Heifei University of Technology, Heifei 230009, China;Engineering Research Center of Bio-process from Ministry of Education, Heifei 230009, China)
出处 《纤维素科学与技术》 CAS CSCD 2019年第2期11-23,共13页 Journal of Cellulose Science and Technology
基金 国家高技术研究发展计划(863计划)项目(SS2014AA021902) 国家自然科学基金青年基金项目(31400049) 安徽省教育厅自然科学研究项目重点项目(非产学研)(2013AJZR0044)。
关键词 疏棉状嗜热霉菌 微波诱变 木聚糖酶 响应面分析法 发酵条件优化 Thermomyces lanuginosus microwave mutagenesis Xylanase response surface analysis optimization of fermentation condition
作者简介 朱慧霞(1977-),女,硕士,副教授;研究方向:生物发酵与酶工程;通讯作者:姚日生(1962-),男,博士,教授;研究方向:制药工程与生物炼制工程。yaors@163.com.
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