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基于信号通路p38 MAPK探讨疏风宣肺汤对大鼠慢性支气管炎模型TNF-α的影响 预览

Exploration on Effects of Shufeng Xuanfei Decoction on TNF-α in Model Rats with Chronic Bronchitis Based on Signaling Pathway p38 MAPK
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摘要 目的观察疏风宣肺汤对慢性支气管炎大鼠血清以及肺组织中肿瘤坏死因子(tumornercrosisfactor-α,TNF-α)的影响。方法将60只SPF级大鼠,随机分为空白组、生理盐水组、宣肺止咳合剂组和疏风宣肺汤组,每组15只;空白组正常饲养,余组采用脂多糖-香烟烟雾诱导法造模成功后分别予以生理盐水、宣肺止咳合剂、疏风宣肺汤灌胃,1周后行腹主动脉采血,剪下右肺,检测血清中TNF-α浓度,肺组织中TNF-αmRNA、TNF-α蛋白以及p-p38MAPK蛋白的表达。结果在药物干预7d后,各组大鼠血清中TNF-α浓度以及肺组织中的TNF-αmRNA、TNF-α蛋白、p-p38MAPK蛋白的表达差异性均有统计学意义(P<0.05)。与空白组相比,疏风宣肺汤组,生理盐水组、宣肺止咳合剂组中各检测指标差异有统计学意义(P<0.05);与生理盐水组相比,疏风宣肺汤组和宣肺止咳含剂组差异有显著性统计学意义(P<0.01或P<0.05)。结论疏风宣肺汤能显著降低慢支大鼠血清中TNF-α浓度以及肺组织TNF-αmRNA、TNF-α蛋白、p-p38MAPK蛋白表达,减少p38MAPK信号通路的活化可能是疏风宣肺汤有效防治慢性支气管炎的机制之一。 Objective To observe the effects of Shufeng Xuanfei Decoction on tumor nercrosis factor-α(TNF-α) in serum and lung tissue of rats with chronic bronchitis. Methods A total of 60 SPF rats were randomly divided into a blank group, a normal saline group, a Xuanfei Zhike Mixture group and a Shufeng Xuanfei Decoction group, with 15 rats in each group. The blank group was fed normally, while the rest groups were given with normal saline, Xuanfei Zhike Mixture and Shufeng Xuanfei Decoction after successful modeling by lipopolysaccharide-cigarette smoke induction method. After 1 week, blood was collected from the abdominal aorta, the right lung was cut off, and the concentration of TNF-α in serum and the expressions of TNF-α mRNA, TNF-α protein and p-p38 MAPK protein in lung tissue were detected. Results After 7 d of medical intervention, the differences in the concentration of TNF-α in serum and the expressions of TNF-α mRNA, TNF-α protein and p-p38 MAPK protein in lung tissue of rats in each group were statistically significant (P<0.05). Furthermore,compared with the blank group, the differences of indexes in the Shufeng Xuanfei Decoction group, the normal saline group and the Xuanfei Zhike Mixture group were statistically significant (P<0.05). Compared with the normal saline group, the difference in the Shufeng Xuanfei Decoction group and the Xuanfei Zhike Mixture group was statistically significant (P<0.01 or P<0.05). Conclusion Shufeng Xuanfei Decoction can significantly reduce the concentration of TNF-α in serum of rats with chronic bronchitis and the expressions of TNF-α mRNA, TNF-α protein and p-p38 MAPK protein in lung tissue. Reducing the activation of p38 MAPK signaling pathway may be one of the effective mechanisms for preventing and treating chronic bronchitis by Shufeng Xuanfei Decoction.
作者 凌嫘 闵锐 范伏元 LING Lei;MIN Rui;FAN Fuyuan(Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China)
出处 《湖南中医药大学学报》 CAS 2019年第6期708-712,共5页 Journal of Hunan University of Chinese Medicine
基金 湖南省中医药科研计划项目(201717).
关键词 疏风宣肺汤 慢性支气管炎 肿瘤坏死因子-Α P38 MAPK信号通路 动物实验 Shufeng Xuanfei Decoction chronic bronchitis TNF-α p38 MAPK signaling pathway animal experiment
作者简介 凌嫘,女,在读硕士研究生,研究方向:风湿免疫学疾病;通讯作者:范伏元,男,教授,博士研究生导师,E-mail:ffy023@163.com.
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