Objective To observe the effects of Shufeng Xuanfei Decoction on tumor nercrosis factor-α(TNF-α) in serum and lung tissue of rats with chronic bronchitis. Methods A total of 60 SPF rats were randomly divided into a blank group, a normal saline group, a Xuanfei Zhike Mixture group and a Shufeng Xuanfei Decoction group, with 15 rats in each group. The blank group was fed normally, while the rest groups were given with normal saline, Xuanfei Zhike Mixture and Shufeng Xuanfei Decoction after successful modeling by lipopolysaccharide-cigarette smoke induction method. After 1 week, blood was collected from the abdominal aorta, the right lung was cut off, and the concentration of TNF-α in serum and the expressions of TNF-α mRNA, TNF-α protein and p-p38 MAPK protein in lung tissue were detected. Results After 7 d of medical intervention, the differences in the concentration of TNF-α in serum and the expressions of TNF-α mRNA, TNF-α protein and p-p38 MAPK protein in lung tissue of rats in each group were statistically significant (P<0.05). Furthermore,compared with the blank group, the differences of indexes in the Shufeng Xuanfei Decoction group, the normal saline group and the Xuanfei Zhike Mixture group were statistically significant (P<0.05). Compared with the normal saline group, the difference in the Shufeng Xuanfei Decoction group and the Xuanfei Zhike Mixture group was statistically significant (P<0.01 or P<0.05). Conclusion Shufeng Xuanfei Decoction can significantly reduce the concentration of TNF-α in serum of rats with chronic bronchitis and the expressions of TNF-α mRNA, TNF-α protein and p-p38 MAPK protein in lung tissue. Reducing the activation of p38 MAPK signaling pathway may be one of the effective mechanisms for preventing and treating chronic bronchitis by Shufeng Xuanfei Decoction.
Journal of Hunan University of Chinese Medicine