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青龙衣膏对胃癌BGC-823细胞侵袭能力的影响 预览

Effects of Qinglongyi Electuary on Invasion Ability of Gastric Cancer BGC-823 Cells
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摘要 目的:探讨青龙衣膏对胃癌BGC-823细胞侵袭能力的影响。方法:采用不同浓度的青龙衣膏处理胃癌BGC-823细胞24、48h,通过噻唑蓝比色法检测青龙衣膏对细胞生长的影响,并计算半数抑制浓度(IC50);以不影响细胞生长的青龙衣膏浓度作为检测其对细胞侵袭能力影响的浓度;将细胞分为对照组、低剂量组(0.66mg/ml)、中剂量组(0.74mg/ml)及高剂量组(0.82mg/ml),处理胃癌24h;采用噻唑蓝比色法检测细胞与胞外基质(ECM)的黏附能力;Transwell小室法检测细胞迁移和侵袭能力;westernblot法检测胃癌BGC-823细胞环氧酶2(COX-2)表达。结果:青龙衣膏可明显抑制胃癌BGC-823细胞增殖,呈现一定的时间-剂量依赖关系。对照组,低、中及高剂量组处理后24h后的细胞黏附率分别为100%、(88.39±2.51)%、(90.23±7.27)%及(81.37±2.0)%;迁移细胞数分别为(134±23.23)、(27.70±5.16)、(28.70±6.27)及(32.00±3.65)个;侵袭细胞数分别为(95.00±9.23)、(13.00±1.10)、(15.10±2.91)及(27.80±4.04)个;且与对照组比较,低、中及高剂量组的COX-2蛋白表达水平明显降低,差异有极显著统计学意义(P<0.01)。结论:青龙衣膏可抑制胃癌BGC-823细胞的侵袭能力,可能与抑制COX-2的表达相关。 OBJECTIVE:To probe into the effects of Qinglongyi electuary on invasion ability of gastric cancer BGC-823 cells.METHODS:The gastric cancer BGC-823 cells were treated with different concentrations of Qinglongyi electuary for 24 and 48 h.The effects of Qinglongyi electuary on cell growth was detected by MTT assay,and the IC 50 value was calculated.The concentration of Qinglongyi electuary,which did not affect the cell growth,was used as the dose to test the effects of Qinglongyi electuary on cell invasion ability.The cells were divided into the control group,the low dose group (0.66 mg/ml),the medium dose group (0.74 mg/ml) and the high (0.82 mg/ml) dose group to treat gastric cancer BGC-823 cells for 24 h.Cell adhesion to extracellular matrix (ECM) was determined by MTT assay.Transwell chamber assay was used to detect the ability of cell migration and invasion.Western blot analysis was adopted to detect the cyclooxygenase 2 (COX-2) expression in gastric cancer cells.RESULTS:Qinglongyi electuary can significantly inhibit the proliferation of gastric cancer BGC-823 cells,showing a certain time-dose dependence.The cell adhesion rates were 100%,(88.39±2.51)%,(90.23±7.27)% and (81.37±2.0)% respectively in the control group,low,medium and high dose group after treatment of 24 h.The number of cell migration was (134±23.23),(27.70±5.16),(28.70±6.27) and (32.00±3.65),respectively.The number of cells invaded was (95.00±9.23),(13.00±1.10),(15.10±2.91) and (27.80±4.04),respectively.Compared with the control group,the expression levels of COX-2 protein in the low,medium and high dose groups were significantly decreased,with extremely statistically significant differences ( P <0.01).CONCLUSIONS:Qinglongyi electuary can inhibit the invasion ability of gastric cancer BGC-823 cells,which may be related to the inhibition of COX-2 expression.
作者 崔红霞 郭雪莹 王明 王伟明 CUI Hongxia;GUO Xueying;WANG Ming;WANG Weiming(Institute of Chinese Medicine,Heilongjiang Academy of Chinese Medicine Sciences,Heilongjiang Harbin 150036,China;School of Pharmacy,Qiqihaer Medical University,Heilongjiang Qiqihar 161006,China)
出处 《中国医院用药评价与分析》 2019年第5期590-593,共4页 Evaluation and Analysis of Drug-use in Hospitals of China
关键词 青龙衣膏 胃癌BGC-823细胞 侵袭 COX-2 Qinglongyi electuary Gastric cancer BGC-823 cells Invasion COX-2
作者简介 崔红霞,教授,硕士生导师。研究方向:肿瘤分子靶向性研究。E-mail:236197163@qq.com;通信作者:王伟明,研究员。研究方向:中药新产品研发。E-mail:zyyjy@163.com.
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