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血清外泌体中HBV标志物检测的临床意义 预览

The clinical value of detection of HBV markers in serum exosome
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摘要 目的检测HBV感染者血清外泌体中HBV成分,比较其与常规检测方法的差异,评价其对检验结果的影响。方法提取HBV感染者血清外泌体,与血清样本一起,用荧光定量PCR法检测HBVDNA,以电化学发光方法检测HBVHBsAg和HBeAg。T检验比较其间差异,分析相关系数。结果血清外泌体中含有大量HBVDNA、HBsAg和HBeAg。其中HBVDNA在血清和外泌体之间差异不显著,但HBsAg和HBeAg在低浓度血清样本中高度聚集于外泌体,最高差异分别可达100倍和25倍。结论血清外泌体中含有大量HBVDNA、HBsAg和HBeAg;HBVDNA在血清和外泌体中接近等量;血清外泌体中HBsAg和HBeAg检测对于HBV感染有重要提示意义。 Objective To detect HBV components in serum exosomes of HBV-infected patients,compare the differences of the results with conventional methods,evaluate its effect on test reports.Methods Extracting serum exosomes from HBV-infected individuals,then,together with serum samples,HBV DNA was deteced using fluorescent quantitative PCR,and HBV HbsAg and HbeAg were measured by using Electrochemiluminescence.Finally,T test was used to compare the differences between them,and the correlation coefficient was further evaluated.Results There were large amounts of HBV DNA,HBsAg,and HBeAg in serum exosomes.No significant difference was observed in HBV DNA between serum and exosomes,but HBsAg and HBeAg was significantly higher as 100 folds and 25 folds respectively in exosomes than that in the serum with lower antigens.Conclusion There were large amounts of HBV DNA,HBsAg,and HBeAg in serum exosomes.HBV DNA is nearly equal in serum and exosomes.The detection of HBsAg and HBeAg in serum exosome may be helpful for diagnosis of HBV infection.
作者 朱琳 辛毅娟 马越云 苏明权 郝晓柯 杨柳 ZHU Lin;XIN Yijuan;MA Yueyun;SU Mingquan;HAO Xiaoke;YANG Liu(Department of Clinical Laboratory Medicine.Xijing Hospital,The Air-force Medical University,Xian 710032,China)
出处 《国际检验医学杂志》 CAS 2019年第A01期112-115,共4页 International Journal of Laboratory Medicine
关键词 乙型肝炎病毒 E抗原 表面抗原 核酸 外泌体 hepatitis B virus HBeAg HBsAg nuclear acid exosome
作者简介 通信作者:杨柳,E-mail:zhangzh@fmmu.edu.cn。
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