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土壤来源抗MRSA放线菌的筛选及次级代谢潜力评估 预览

Screening and potential evaluation of secondary metabolic of anti-MRSA Actinomycetes from soil
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摘要 该研究采用4种分离培养基对32份土壤样本中的放线菌进行初步分离纯化,通过抗耐甲氧西林金黄色葡萄球菌(MRSA)抑菌活性实验进行菌株初筛,并采用聚合酶链式反应(PCR)扩增菌株16S rRNA和次级代谢相关基因,对活性菌株的代谢潜力进行评估。结果表明,从32份样本中共分离纯化到169株放线菌,其中68株放线菌具有抗MRSA活性。活性菌株主要为链霉菌属(Streptomyces sp.),且16S rRNA基因序列相似性<98%的菌株有10株,卤化酶(Hal)基因阳性菌株占17.65%,聚酮合酶Ⅰ型(PKS Ⅰ)基因阳性菌株占36.47%,聚酮合酶Ⅱ(PKS Ⅱ)基因阳性菌株占61.77%,非核糖体多肽合成酶(NRPS)基因阳性菌株占32.35%。表明黔北地区土样中放线菌资源丰富,具有从中发现放线菌新种和抗生素的潜力。 The Actinomycetes were primary isolated and purified from 32 soil samples using four separation media. The strains were screened out by anti-methicillin-resistant Staphylococcus aureus (MRSA) bacteriostatic test,and the 16S rRNA and secondary metabolism-related genes were amplified by polymerase chain reaction to evaluate the metabolic potential of the active strain. The results showed that 169 strains of Actinomycetes were isolated and purified from 32 samples. Among them,68 strains of Actinomycetes had anti-MRSA activity. The active strains were mainly Streptomyces sp.,and there were 10 strains with 16S rRNA gene sequence similarity less than 98%;halogenase (Hal) gene-positive strains accounted for 17.65%,polyketide synthase type I (PKS I) gene-positive strains accounted for 36.47%,polyketide synthase II (PKS II) gene-positive strains accounted for 61.77%,and non-ribosomal polypeptide synthetase (NRPS) gene-positive strains accounted for 32.35%. It was indicated that the Actinomycetes were rich in soil samples in the northern Guizhou province of China,and had the potential to discover new Actinomycetes and antibiotics with novel structure.
作者 梁大敏 田鹏 刘铁 吴德静 岳昌武 LIANG Damin;TIAN Peng;LIU Tie;WU Dejing;YUE Changwu(Zunyi Medical and Pharmaceutical College,Zunyi 563003,China;Zunyi Key Laboratory of Precise Diagnosis and Drug Targeted Therapy of Genetic Diseases,The First People's Hospital of Zunyi & Central Laboratory of The Third Affiliated Hospital of Zunyi Medical University,Zunyi 563003,China;School of Medicine,Yan'an University,Yan'an 716000,China)
出处 《中国酿造》 CAS 北大核心 2019年第7期131-135,共5页 China Brewing
基金 中科院微生物研究所微生物资源前期开发国家重点实验室开放课题(SKLMR-20160601) 贵州省科学技术基金(黔科合基础(2017)1218) 遵义市汇川区科技计划项目(遵汇科合201825) 遵义市科技局基金项目(遵市科合社字(2017)27号、遵市科合社字(2017)36号、遵市科合社字(2017)53号) 贵州省卫计委科学基金项目(gzwjkj-2017-1-048、gzwjkj-2018-2-008) 延安大学科研启动基金(YDBK2018-50).
关键词 放线菌 抗耐甲氧西林金黄色葡萄球菌 筛选 鉴定 次级代谢产物 基因勘探 Actinomycetes anti-methicillin-resistant Staphylococcus aureus screening identification secondary metabolite products gene exploration
作者简介 梁大敏(1987-),女,讲师,硕士,研究方向为分子生物学;通讯作者:岳昌武(1975-),男,教授,博士,研究方向为微生物天然产物发现及生物合成.
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