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mTOR通路抑制剂GDC-0349对CCRF-CEM细胞增殖和凋亡的影响 预览

Effect of GDC-0349,the mTOR pathway inhibitor,on proliferation and apoptosis of CCRF-CEM cells
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摘要 目的:探讨mTOR通路抑制剂GDC-0349对急性T淋巴细胞白血病细胞株CCRF-CEM细胞增殖和凋亡的影响。方法:不同浓度GDC-0349作用于CCRF-CEM细胞,采用CCK-8法检测细胞增殖;流式细胞术检测细胞凋亡率;Westernblot检测凋亡相关蛋白及AKT/mTOR通路相关蛋白的表达。结果:GDC-0349作用于CCRF-CEM细胞后细胞增殖率减少且凋亡率增加(P<0.05);凋亡相关蛋白Caspase3和BCL2下调,Cleaved-Caspase3增加(P<0.05);通路相关蛋白AKT、p-AKT及p-mTOR下调(P<0.05)。结论:GDC-0349能够抑制AKT/mTOR通路活化,从而促进CCRF-CEM细胞凋亡,抑制细胞增殖。 Objective:To investigate the effect of GDC-0349,the mTOR pathway inhibitor,on proliferation and apoptosis of acute T lymphocytic leukemia cell line CCRF-CEM.Methods:Different concentrations of GDC-0349 were applied to CCRF-CEM cells.Cell proliferation was detected by CCK-8 method.Apoptosis rate was detected by flow cytometry.Expressions of apoptosis-related proteins and AKT/mTOR pathway proteins were detected by Western blot.Results:In CCRF-CEM cells with GDC-0349,the cell proliferation rate decreased and the apoptotic rate increased(P<0.05).Caspase3 and BCL2 were down-regulated(P<0.05)and Cleaved-Caspase3 was up-regulated(P<0.05).The expressions of AKT/mTOR pathway-related proteins were down-regulated,such as AKT,p-AKT and p-mTOR(P<0.05).Conclusion:GDC-0349 can inhibit the AKT/mTOR pathway,then promote cell apoptosis and inhibit cell proliferation in CCRF-CEM cells.
作者 李川 胡荣 刘卓刚 Li Chuan;Hu Rong;Liu Zhuogang(Department of Hematology,Shengjing Hospital,China Medical University,Liaoning Shenyang 110031,China)
出处 《现代肿瘤医学》 CAS 2019年第16期2803-2806,共4页 Journal of Modern Oncology
基金 国家自然科学基金资助项目(编号:81500135).
关键词 mTOR通路抑制剂 GDC-0349 急性T淋巴细胞白血病 细胞增殖 凋亡 mTOR pathway inhibitor GDC-0349 acute T lymphocytic leukemia cell proliferation apoptosis
作者简介 李川(1992-),女,云南昭通人,在读硕士研究生,主要从事恶性血液病的诊断与治疗研究。E-mail:lic6450@163.com;通讯作者:刘卓刚(1958-),男,辽宁沈阳人,教授,博士生导师,主任医师,主要从事恶性血液病的诊断与治疗研究。E-mail:liuzg@sj-hospital.org.
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