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基于转录组测序对紫花苜蓿铅胁迫相关基因的富集分析 预览

Enrichment analysis of genes that are related to lead stress in alfalfa, based on transcriptome sequencing
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摘要 本文采用高通量Illumina Hiseq 测序平台对紫花苜蓿(Medicago sativa)的铅胁迫处理组(Pb96)和对照组(CK)进行转录组测序,并对测序数据进行分析,探究紫花苜蓿抗铅应答的分子机制。结果表明,在铅胁迫处理下共检测到2 242 个差异表达基因(Differentially expressed genes,DEGs)。其中,1 321 个DEGs 上调表达,921 个DEGs 下调表达。GO (Gene ontology,http://www.geneontology.org/)富集分析,差异表达基因主要涉及物质代谢、蛋白结合、催化活性等。KEGG (Kyoto encyclopedia of genes and genomes,http://www.genome.jp/kegg/)分析,差异基因主要涉及代谢途径、光合作用、淀粉和蔗糖代谢等。COG (Clusters of orthologous groups of proteins/orthologous groups of genes)分析,差异基因主要涉及“碳水化合物的运输和新陈代谢”、“翻译、核糖体结构和生物发生”、“次生代谢产物的生物合成、转运和分解代谢”等。本研究通过对紫花苜蓿根系转录组分析,为研究紫花苜蓿耐铅分子机制提供参考。 This paper used a high-throughput Illumina Hiseq sequencing platform to sequence the transcriptome of the control group (CK) and drought treatment group (Pb96) of alfalfa (Medicago sativa), and analyzed the sequencing data to further explore the molecular mechanism of alfalfa resistance to lead. The results showed that 2 242 differentially expressed genes (DEGs) were detected under lead stress treatment. Among them, 1 321 DEGs were up-regulated and 921 were downregulated. Within GO (gene ontology, http://www.geneontology.org/) enrichment analysis, differential genes are mainly involved in material metabolism, protein binding, and catalytic activity. According to KEGG (Kyoto encyclopedia of genes and genomes, http://www.genome.jp/kegg/) analysis, differential genes are mainly involved in metabolic pathways, photosynthesis, and starch sucrose metabolism. In COG (clusters of orthologous groups of proteins/orthologous groups of genes) analysis, differential genes are mainly involved in carbohydrate transport and metabolism, translation, ribosome structure and biogenesis, biosynthesis of secondary metabolites, transport, and catabolism. This study provides a reference for studying the molecular mechanism of alfalfa resistance to lead by transcriptome analysis of alfalfa roots.
作者 郭强 王英哲 王昆 徐博 GUO Qiang;WANG Yingzhe;WANG Kun;XU Bo(College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, Jilin, China;Jilin Academy of Agricultural Sciences, Changchun 130118, Jilin, China;Animal Husbandry and Veterinary Department, 291 Farm of Heilongjiang Province Heilongjiang, Jixian 155923, Heilongjiang, China)
出处 《草业科学》 CAS CSCD 2019年第10期2525-2534,共10页 Pratacultural Science
基金 吉林省科技厅科技支撑计划“优质饲草品种选育及其配套种植技术研究与示范”(20180201072ny) 吉林省教育厅科学技术研究项目“紫花苜蓿细胞质雄性不育系的细胞学和生理生化特性的研究”(JJKH20180666KJ).
关键词 紫花苜蓿 转录组 铅胁迫 耐铅基因 alfalfa transcriptome lead stress lead-tolerant gene
作者简介 第一作者:郭强(1993-),男,黑龙江大庆人,在读硕士生,研究方向为饲草种质资源开发与利用。E-mail:278315633@qq.com;通信作者:徐博(1983-),男,吉林公主岭人,讲师,博士,研究方向为饲草种质资源开发与利用。E-mail:xubo0308@126.com.
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