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外周血端粒酶活性对肺癌血转移诊断的探讨 预览

Study on the diagnostic value of peri pneral blood's telomerase activity in lung cancer
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摘要 目的探讨以端粒酶重复序列扩增法(PCR-TRAP)检测血中肺癌端粒酶活性对于肺癌血转移诊断、分型分期的临床意义.方法选取24例肺癌、26例肺良性疾病患者,应用PCR-TRAP法测定端粒酶活性,磁分离酶免法检查癌胚抗原(CEA)及血沉.结果肺癌组端粒酶活性阳性率明显高于肺良性疾病组(p<0.01);按肺癌恶化程度分,Ⅲ-Ⅳ期比Ⅰ-Ⅱ期高.CEA和血沉在肺癌与肺良性疾病组异常率无显著性差异(p>0.05).22.2%(4/18)晚期肺癌外周血端粒酶活性呈阴性,肺良性疾病患者中19.2%(5/26)外周血端粒酶活性呈阳性,肺癌患者外周血端粒酶活性敏感性比CEA、ESR高(p<0.01和p<0.05),特异性无显著性差别(p>0.05).结论端粒酶对于肺癌分期分型及预后判断有临床应用价值.改进检测方法,对提高肺癌血转移诊断的特异性和敏感度显得重要. Objective To explore the significance of telomerase activity in lung cancer by PCR TRAP and its relationship with the metastasis and gradation of lung cancer. Methods Telomerase activity was measured and compared in twenty four patients with lung cancer and twenty six patients with lung benign lesion, using three different methods TRAP, CEA and ESR. Results Telomerase positive rate was significantly higher in patients with lung cancer than that in patients with benign lung lesion (p<0.01);Stage III to IV is higher than stage I and II. There are no significant difference in the detection of CEA and ESR between lung cancer and benign lesion (p>0.05). The negative rate of telomerase was 22.2% (4/18) in advanced lung cancer. The positive rate of telomerase was 19.2% (5/26) in lung benign lesion. Sensitivity of pneral blood's telomerase activity in lung cancer was higher than that of CEA and ESR (p>0.05). There were no significant difference in the specificity (p>0.05). Conclusion Telomerase activity might be a tumor marker for detecting lung cancer cells in peripheral blood. The detection of telomerase was effective in the diagnosis and gradation of lung cancer. It might improve the specificity and sensitivity of diagnosis of lung cancer by further improvement of detecting method.
作者 舒国长 吴南茜 张庆五 冯久贤 Shu Guochang WU Nanxi Zhang Qingwu et al Shanghai Kongjiang Hospital, Shanghai 200093
出处 《临床肺科杂志》 2003年第3期 207-208,共2页 Journal of Clinical Pulmonary Medicine
关键词 外周血 端粒酶 活性 肺癌 血转移诊断 Lung cancer Telomerase Tumor markers
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