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探讨TRUST和TPPA法联合检测梅毒螺旋体抗体的临床价值 预览

The clinical value of combined TRUST and TPPA detection method in detecting treponema pallidum antibodies
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摘要 目的探讨酶联免疫吸附试验(ELISA)、甲苯胺红不加热血清实验(TRUST)、梅毒螺旋体明胶颗粒凝集试验(TPPA)以及联合检验对梅毒螺旋体(Treponema pallidum,TP)抗体的临床价值。方法选取2015年1月—2017年12月来本院进行检查的287例疑似梅毒患者作为研究对象,先行ELISA法进行TP抗体血清学筛查,再行TRUST和TPPA法检测,以《梅毒诊断标准》进行确诊,比较三种检查方法的诊断准确率以及联合检测的诊断价值。结果 ELISA法和TPPA法对疑似梅毒的诊断准确率(分别为93.38%和95.82%)均高于TRUST法(84.32%),差异有统计学意义(P<0.05);三种检查方法对梅毒诊断的灵敏度在68.97%~94.83%之间,特异度在94.74%~96.49%之间,约登指数在0.637~0.913之间;ELISA法筛查的基础上,进行TRUST+TPPA联合检测,其灵敏度、特异度和约登指数均较高,分别为98.28%、94.74%和0.930。结论 ELISA法和TPPA法对疑似梅毒的诊断符合率较高,三种方法联合检测的诊断价值高,可先行ELISA法筛查,再进行TRUST法和TPPA法联合检测提高梅毒诊断的临床价值。 Objective To explore the clinical value of enzyme-linked immunosorbent assay(ELISA),tolulized red unheated serum test(TRUST),treponema pallidum particle assay(TPPA)and combined detection of them for treponema pallidum antibodies.Methods 287 patients of suspected syphilis,visit our hospital for examination during January 2015 and December 2017,were selected as study subjects.They were screened for TP antibody by ELISA,followed detected by TRUST and TPPA.All patients were diagnosed according to the Syphilis Diagnostic Criteria.The diagnostic accuracy of the three test methods and the diagnostic value of the combined test were compared.Results The diagnostic accuracy of ELISA and TPPA(93.38%and 95.82 respectively)for suspected syphilis was significantly higher than the TRUST method(84.32%),P<0.05.The sensitivity of the three test methods for the syphilis diagnosis was between 68.97%and 94.83%,the specificity was between 94.74%and 96.49%,and the Yoden index was between 0.637 and 0.913.On the basis of ELISA screening,the sensitivity,specificity and Yoden index were 98.28%,94.74%and 0.930 respectively in the combined detection of TRUST and TPPA.Conclusions The diagnostic rate of ELISA and TPPA for suspected syphilis was relatively high,and the diagnostic value of combined detection of the three methods was high.The patients of suspected syphili could be screened by ELISA,and then detected by combined detection of TRUST and TPPA,in order to improve the clinical value in diagnosing syphilis diagnosis.
作者 林明春 肖礼红 赵洪民 夏凤琼 LIN Ming-chun(Orthopedic hospital of Guizhou,Guiyang,Guizhou,550007,China)
机构地区 贵州省骨科医院
出处 《齐齐哈尔医学院学报》 2019年第6期699-701,共3页 Journal of Qiqihar Medical College
关键词 梅毒螺旋体 ELISA TRUST TPPA 诊断价值 Treponema pallidum ELISA TRUST TPPA Diagnostic value
作者简介 通信作者:林明春,Email:29839794@qq.com
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