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金银花中总黄酮和绿原酸加压同步提取的工艺优化 预览

Optimization of Synchronization Extraction Process of Total Flavonoids and Chlorogenic Acid by Pressure Assisted from Lonicerae japonicae
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摘要 本文旨在探索加压辅助同步提取金银花中总黄酮和绿原酸的工艺。在单因素的基础上进行正交试验,考察了甲醇浓度、料液比、提取压力、提取时间和提取温度因素对总黄酮和绿原酸得率的影响。结果表明,提取压力对金银花中两类化学成分的提取有显著影响(p <0.05);优化工艺条件为甲醇浓度60%、料液比1∶20 (g/m L)、提取压力2 MPa、提取时间30 min、提取温度70℃;工艺条件稳定,与超声波辅助提取法相比,提取效率更高。在此试验条件下,总黄酮和绿原酸的得率分别为15.66%和3.89%。本研究为金银花的开发和利用提供了新的理论依据。 This study was to explore the process of simultaneous extracting total flavonoids and chlorogenic acid from Lonicerae japonicae by pressurization assisted.Based on the single factor experiment,orthogonal experiments were carried out to investigate the effects of solvent concentration,liquid ratio,extraction pressure,extraction time and extraction temperature on the extraction rate of total flavonoids and chlorogenic acid.The results showed that the extraction pressure had a significant effect( p < 0.05) on the extraction of two kinds of chemical constituents from Lonicerae japonicae.The optimum process conditions were as follows:Methanol concentration was 60%,solid-liquid ratio was 1 ∶ 20( g/m L),extraction pressure was 2 MPa,extraction time was30 min,extraction temperature was 70 ℃.The process conditions were stable,and the extraction efficiency was higher than that of ultrasonic assisted extraction method.Under the test conditions,the extraction rates of total flavonoids and chlorogenic acids were respectively 15.66% and 3.89%. This study provided a new theoretical basis for the development and utilization of Lonicerae japonicae.
作者 李华生 周振华 张航航 杨春华 陈瑛 LI Hua-sheng;ZHOU Zhen-hua;ZHANG Hang-hang;YANG Chun-hua;CHEN Ying(Yongzhou Vocational and Technical College,Yongzhou 425100,China)
出处 《食品工业科技》 CAS 北大核心 2019年第4期172-177,共6页 Science and Technology of Food Industry
基金 湖南省教育厅2017年科研项目(17C1603).
关键词 金银花 加压辅助提取 总黄酮 绿原酸 Lonicerae japonicae pressure-assisted extraction total flavonoids chlorogenic acid
作者简介 李华生(1972-),男,硕士,讲师,研究方向:天然药用植物化学成分的提取、分离和分析,E-mail:lthuashen@163.com。
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