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HPLC法同时测定白斑膏中4种成分的含量 预览 被引量:3

Simultaneous determination of the contents of the four components in the Baiban Cream by HPLC
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摘要 目的建立白斑膏中4种成分含量测定方法.方法采用HPLC法测定白斑膏中克霉唑、氢化可的松、己烯雌酚、马来酸氯苯那敏含量.选用Diamonsil C18柱( 150 mm× 4.6 mm, 5 μm),流动相为甲醇-水-乙腈-三乙胺(体积比65∶25∶10∶0.2),流速1.0 mL*min-1,检测波长 260 nm.结果该方法不受处方中基质成分干扰,克霉唑在0.54~ 1.26 g*L-1、氢化可的松在30.0~ 70.0 mg*L-1、己烯雌酚在0.9~ 2.1 mg*L-1、马来酸氯苯那敏在5.4~ 12.6 mg*L-1内线性关系良好(rclotrimazole= 0.999 9,rhydrocortisone= 0.999 6,rdiethylstilbestrol= 0.999 8,rchlorphenamine= 0.999 6),平均加样回收率:克霉唑99.5%、氢化可的松99.6%、己烯雌酚99.2%、马来酸氯苯那敏100.1%.RSD分别为1.1%、1.3%、0.89%、1.5%(n=5).结论该方法可以控制白斑膏的质量. Objective To develop a method for the determination of the contents of the four components in the Baiban Cream. Methods Clotrimazole, hydrocortisone, diethylstilbestrol, chlorphenamine maleate in the Baiban Cream were determined by high-performance liquid chromatography with the detection wavelength at 260 nm and were eluted with methanol-water-acetonirile-triethylamine (65∶25∶10∶0.2 ) on C 18 column.Results The calibration curve was liner within the range of 0.54~1.26 g·L -1 for clotrimazole ( r= 0.999 9),30.0~ 70.0 mg·L -1 for hydrocortisone (r= 0.999 6),0.9~ 2.1 mg·L -1 for diethylstilbestrol (r= 0.999 8) and 5.4~ 12.6 mg·L -1 for chlorphenamine maleate(r= 0.999 6).The average recoveries of the added components in samples of clotrimazole, hydrocortisone, diethylstilbestrol and chlorphenamine maleate were 99.5%,99.6%,99.2%,100.1% respectively. RSD were 1.1%,1.3%,0.89%,1.5% respectively.Conclusion The method can be used for the quality control of the Cream.
作者 徐英宏 菅凌燕 赵铁 段旭 XU Ying-hong, JIAN Ling-yan, ZHAO Tie, DUAN Xu (The 2nd Hospital of China Medical University, Shengyang 110004, China)
出处 《沈阳药科大学学报》 CAS CSCD 2003年第6期 442-444,共3页 Journal of Shenyang Pharmaceutical University
关键词 高效液相色谱法 白斑膏 克霉唑 氢化可的松 己烯雌酚 马来酸氯苯那敏 HPLC Baiban Cream clotrimazole hydrocortisone diethylstilbestrol chlorphenamine maleate
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