目的:研究肌损伤浸液作用于骨髓间充质干细胞( mesenchymal stem cells,MSCs)对其增殖的影响以及成肌诱导作用. 方法:分离、扩增小鼠骨髓 MSCs,以切割伤及液氮冻伤法建立肌损伤并提取伤后 8～ 72 h的伤浸液,以 Bradford比色法检测其蛋白含量并选取含量最高的时相组,以四唑盐( MTT)法检测该组伤浸液对 MSCs增殖的影响,以反转录聚合酶链反应( reverse transcription-polymerase chain reaction,RT-PCR)法检测其作用后生肌调控因子中生肌素( myogenin)的表达. 结果 :肌损伤后 12 h时的伤浸液蛋白含量最高;作用于 MSCs后可明显增强 MSCs的增殖能力,但并不能诱导 MSCs表达 myogenin. 结论 :肌损伤浸液可促进 MSCs的增殖但对 MSCs无成肌诱导分化作用.
AIM:To study the influence of muscle-injury wound fluid on the proliferation and myogenic differentiation ability of bone marrow-derived mesenchymal stem ce lls (MSCs). METHODS: Bone marrow-derived MSCs of mouse were seperated and purified; muscl e injury were created by using incising injury and freezing injury with liquid n itrogen and wound fluid was collected in the 8-72 hours after damaged. Their pr oteins concentration were measured with Braford colorimetry method and choosed t he higest concentration group; the influence of this group wound fluid on the pr oliferation of MSCs was detected with microculture tetrozolium(MTT) method; the expression of myogenin on MSCs was detected with reverse transcription-polymera se chain reaction(RT-PCR) method. RESULTS:The higest concentration of wound fluid was at the 12 hours, after eff ected on MSCs it could enhance the proliferation ability of MSCs but could not i nduce MSCs to express myogenin.
Chinese Journal of Clinical Rehabilitation
Muscle-injury wound fluid can promote MSCs proliferation but have no effect on inducing MSCs to differentiate into myoblasts.