期刊文献+
共找到21,505篇文章
< 1 2 250 >
每页显示 20 50 100
Intravitreal conbercept injection for neovascular agerelated macular degeneration
1
作者 Bing-Hui Wu Bing Wang +2 位作者 Hui-Qin Wu Qin Chang Hui-Qin Lu 《国际眼科杂志:英文版》 SCIE CAS 2019年第2期252-257,共6页
AIM:To evaluate the efficacy and safety of intravitreal injection of conbercept in patients with neovascular agerelated macular degeneration(AMD). METHODS:Retrospective review of 66 eyes of 63 patients with neovascula... AIM:To evaluate the efficacy and safety of intravitreal injection of conbercept in patients with neovascular agerelated macular degeneration(AMD). METHODS:Retrospective review of 66 eyes of 63 patients with neovascular AMD. All patients received 0.5 mg intravitreal injections of conbercept monthly for 3 consecutive months, and then pro re nata treatment was performed. The changes of best-corrected visual acuity(BCVA) and central macular thickness(CMT) were observed before and after treatments. Minimum follow-up time was 12 mo. SPSS 22.0 statistical software was used for statistical analysis. RESULTS:The mean BCVA and CMT of 66 eyes(63 patients) were 1.11±0.60, 533.20±219.95 μm at baseline, and were 0.68±0.38, 310.28±125.60 μm at 3 mo. No subjects were lost during the first three months, the improvements were all significantly(P<0.05). During the whole follow-up time of 12 mo, 15 subjects(18 eyes) were lost. The mean BCVA and CMT of the rest 48 eyes with the follow-up time at least 1 y were 0.83±0.46 and 547.59±196.77 μm at baseline, after 3 mo and 12 mo of conbercept injections became 0.55±0.41, 318.24±141.29 μm and 0.55±0.51, 333.87±173.25 μm. The differences were significant(P<0.05). No serious complications were observed. CONCLUSION:Intravitreal injection of conbercept appears to significantly improve visual acuity and anatomical outcomes in patients with neovascular AMD, no serious adverse reactions and complications are observed. 展开更多
关键词 AGE-RELATED MACULAR DEGENERATION INTRAVITREAL INJECTION conbercept
IgA肾病患者肾间质血管病变的临床及病理意义 预览
2
作者 张国庆 王慧 +3 位作者 李博 保莉 陆晓华 郑亚莉 《宁夏医学杂志》 CAS 2019年第4期298-300,共3页
目的分析IgA肾病患者肾间质血管病变的临床及病理特点。方法将98例肾活检为原发性IgA肾病住院患者根据是否发生肾间质血管病变(血管壁增厚和/或玻璃样变),分成血管病变组和无血管病变组。分析2组患者间临床及病理指标的特点与关系。结... 目的分析IgA肾病患者肾间质血管病变的临床及病理特点。方法将98例肾活检为原发性IgA肾病住院患者根据是否发生肾间质血管病变(血管壁增厚和/或玻璃样变),分成血管病变组和无血管病变组。分析2组患者间临床及病理指标的特点与关系。结果血管病变组患者收缩压、血清肌酐、尿素氮、血尿酸、甘油三酯及24 h尿蛋白定量明显高于无血管病变组患者(P<0.05)。血管病变组出现肾小管萎缩或间质纤维化、新月体及肾小球硬化的比例明显高于无血管病变组患者(P<0.05)。血管病变组患者Lee’s分级高于无血管病变组(P<0.05)。结论伴有肾间质血管病变的IgA肾病患者,其临床指标及病理损害更为严重,肾间质血管病变可以作为判断IgA肾病预后的一项重要病理指标。 展开更多
关键词 IGA肾病 肾间质血管病变 病理
在线阅读 下载PDF
β-(1-取代吲哚基)-β-乙硫基-α,β-不饱和酮的有效合成 预览
3
作者 魏威 赵辉 +5 位作者 路博 魏思静 姜诗雨 赵晖 刘洋 于海丰 《化学研究与应用》 CAS CSCD 北大核心 2019年第5期824-832,共9页
本文研究了β-(1-取代吲哚基)-β-乙硫基-α,β-不饱和酮的有效合成。研究表明,在三氟乙酸(TFA)介质中,回流条件下,α-羰基二硫缩烯酮与1-取代吲哚(摩尔比6∶5)有效发生脱硫C-C偶联反应,高产率合成β-(1-取代吲哚基)-β-乙硫基-α,β-... 本文研究了β-(1-取代吲哚基)-β-乙硫基-α,β-不饱和酮的有效合成。研究表明,在三氟乙酸(TFA)介质中,回流条件下,α-羰基二硫缩烯酮与1-取代吲哚(摩尔比6∶5)有效发生脱硫C-C偶联反应,高产率合成β-(1-取代吲哚基)-β-乙硫基-α,β-不饱和酮。 展开更多
关键词 吲哚 二硫缩烯酮 Α Β-不饱和酮 偶联反应
在线阅读 下载PDF
地黄饮子方结合胞磷胆碱治疗血管性痴呆肾精亏虚兼痰瘀阻络证疗效及对血清β-AP、E 2、Livin水平的影响 预览
4
作者 覃辉 周城恩 +2 位作者 陆晖 江颖 张永全 《现代中西医结合杂志》 CAS 2019年第6期589-592,596共5页
目的观察地黄饮子方结合胞磷胆碱治疗血管性痴呆肾精亏虚兼痰瘀阻络证疗效及对血清β淀粉样蛋白(β-AP)、雌二醇(E2)及抗凋亡因子(Livin)水平的影响。方法将96例血管性痴呆肾精亏虚兼痰瘀阻络证患者随机分为观察组和对照组各48例。对照... 目的观察地黄饮子方结合胞磷胆碱治疗血管性痴呆肾精亏虚兼痰瘀阻络证疗效及对血清β淀粉样蛋白(β-AP)、雌二醇(E2)及抗凋亡因子(Livin)水平的影响。方法将96例血管性痴呆肾精亏虚兼痰瘀阻络证患者随机分为观察组和对照组各48例。对照组给予胞磷胆碱钠胶囊口服治疗,观察组给予地黄饮子方结合胞磷胆碱钠胶囊治疗。治疗3个月后,对比2组认知功能(记忆力、定向力、计算力和注意力、语言能力、MMSE总分)、生活自理能力(ADL评分)、精神病理症状(NPI评分)及血清β-AP、E2、Livin水平的改善情况,并评估临床疗效。结果观察组治疗后总有效率明显高于对照组(P<0.05);2组MMSE总分,记忆力、定向力、计算力和注意力、语言能力积分,E2水平、Livin水平及ADL评分均显著升高(P均<0.05),NPI评分和β-AP水平均显著降低(P均<0.05),且观察组治疗后上述指标改善程度更为明显(P均<0.05)。结论地黄饮子方结合胞磷胆碱治疗血管性痴呆肾精亏虚兼痰瘀阻络证,能够有效改善患者的认知功能、生活自理能力、精神病理状态以及血清β-AP、E2和Livin表达水平,疗效确切。 展开更多
关键词 血管性痴呆 肾精亏虚兼痰瘀阻络证 地黄饮子方 胞磷胆碱 认知功能
在线阅读 下载PDF
Application and prospects of butylphthalide for the treatment of neurologic diseases
5
作者 Xi-Qian Chen Ke Qiu +3 位作者 Hui Liu Qiang He Jia-Hui Bai Wei Lu 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第12期1467-1477,共11页
Objective: The 3-N-butylphthalide (NBP) comprises one of the chemical constituents of celery oil. It has a series of pharmacologic mechanisms including reconstructing microcirculation, protecting mitochondrial functio... Objective: The 3-N-butylphthalide (NBP) comprises one of the chemical constituents of celery oil. It has a series of pharmacologic mechanisms including reconstructing microcirculation, protecting mitochondrial function, inhibiting oxidative stress, inhibiting neuronal apoptosis, etc. Based on the complex multi-targets of pharmacologic mechanisms of NBP, the clinical application of NBP is increasing and more clinical researches and animal experiments are also focused on NBP. The aim of this review was to comprehensively and systematically summarize the application of NBP on neurologic diseases and briefly summarize its application to non-neurologic diseases. Moreover, recent progress in experimental models of NBP on animals was summarized. Data sources: Literature was collected from PubMed and Wangfang database until November 2018, using the search terms including "3-N-butylphthalide,""microcirculation,""mitochondria,""ischemic stroke,""Alzheimer disease,""vascular dementia,""Parkinson disease,""brain edema,""CO poisoning,""traumatic central nervous system injury,""autoimmune disease,""amyotrophic lateral sclerosis,""seizures,""diabetes,""diabetic cataract," and "atherosclerosis." Study selection: Literature was mainly derived from English articles or articles that could be obtained with English abstracts and partly derived from Chinese articles. Article type was not limited. References were also identified from the bibliographies of identified articles and the authors’ files. Results: NBP has become an important adjunct for ischemic stroke. In vascular dementia, the clinical application of NBP to treat severe cognitive dysfunction syndrome caused by the hypoperfusion of brain tissue during cerebrovascular disease is also increasing. Evidence also suggests that NBP has a therapeutic effect for neurodegenerative diseases. Many animal experiments have found that it can also improve symptoms in other neurologic diseases such as epilepsy, cerebral edema, and decreased cognitive function caused by severe acute carbon 展开更多
关键词 3-N-BUTYLPHTHALIDE PHARMACOLOGICAL mechanisms MICROCIRCULATION MITOCHONDRIA ISCHEMIC stroke
实时三维超声心动图检测左室质量指数评价主动脉瓣置换术后左心室重构的改善情况 预览
6
作者 童仙君 曹慧 +4 位作者 熊文峰 程遵华 金露 万文婷 孙辉 《实用医学杂志》 CAS 北大核心 2019年第13期2161-2165,共5页
目的探索实时三维超声心动图(RT-3DE)计算左室质量指数(LVMI)评估主动脉瓣置换(AVR)前后主动脉瓣病变患者左室重构(LVH)的改善情况。方法选择行AVR 的主动脉瓣疾病患者并定义为病例组(n=68),其中主动脉瓣狭窄(AS)33例,主动脉瓣关闭不全(... 目的探索实时三维超声心动图(RT-3DE)计算左室质量指数(LVMI)评估主动脉瓣置换(AVR)前后主动脉瓣病变患者左室重构(LVH)的改善情况。方法选择行AVR 的主动脉瓣疾病患者并定义为病例组(n=68),其中主动脉瓣狭窄(AS)33例,主动脉瓣关闭不全(AI)35例。同期选择健康体检人群作为对照组(n=70)。分别于AVR 前,治疗后1 周、1 个月和6 个月进行超声心动图检查并计算2D-LVMI 和3D-LVMI。所有检查均由2 名超声医师采用单盲独立测量。采用重复测量方差分析比较AS和AI患者2D-LVMI和3D-LVMI的变化差异。采用Bland-Altman 分析和组内相关系数(ICC)对2D-LVMI和3D-LVMI进行重复性检验。结果AS与AI患者的3D-LVMI和2D-LVMI在整个AVR治疗周期中均呈下降趋势,在不同时间段的变化差异有统计学意义(P<0.001)。组间比较发现,AS患者2D-LVMI大于3D-LVMI,差异有统计学意义(P=0.043)。AI与AS患者在AVR术后1周时3D-LVMI低于2D-LVMI,差异有统计学意义(P<0.05)。3D-LVMI在观察者之间ICC结果为0.966;2D-LVMI的ICC结果为0.757,低于3D-LVMI的一致性。结论RT-3DE能够通过LVMI精确评估主动脉瓣病变患者AVR前后LVH的改善情况。 展开更多
关键词 实时三维超声心动图 主动脉瓣置换 左室质量指数 左室重构
在线阅读 免费下载
神经纤维瘤病1型并矮小症4例临床特点及文献复习
7
作者 韩慧侨 潘慧 +5 位作者 王林杰 阳洪波 陈适 卢琳 苗卉 朱惠娟 《中华实用儿科临床杂志》 CSCD 北大核心 2019年第12期946-949,共4页
目的总结神经纤维瘤病1型(NF1)并矮小症患儿的临床特点。方法回顾性分析4例NF1并矮小症患儿的临床特点及基因突变类型。总结我国已报道的NF1患儿的临床表现和基因突变特点。结果4例患儿(男:女=1:1)均以身材矮小为主诉就诊,确诊为NF1,年... 目的总结神经纤维瘤病1型(NF1)并矮小症患儿的临床特点。方法回顾性分析4例NF1并矮小症患儿的临床特点及基因突变类型。总结我国已报道的NF1患儿的临床表现和基因突变特点。结果4例患儿(男:女=1:1)均以身材矮小为主诉就诊,确诊为NF1,年龄3.6~17.5岁,3例伴牛奶咖啡斑,2例伴腹股沟雀斑,1例伴皮肤神经纤维瘤。4例患儿有3种突变形式,1例NF1基因exon2- exon15大片段重复,2例错义突变,分别为c.3352A>G(p.Ser1118Gly)和c.5425C>T(p.Arg1809Cys),1例移码突变为c.1756_ 1759delACTA(p.Thr586Valfs*18)。据文献报道,中国NF1患儿最常见的临床表现为牛奶咖啡斑(97.9%),最常见的基因突变类型为移码突变(28.4%),身材矮小占10.1%。结论NF1患儿因皮肤表现就诊皮肤专科时,应重视其生长发育情况,而其他专科也应当警惕矮小症患儿中NF1的可能性。 展开更多
关键词 神经纤维瘤病1型 神经纤维蛋白1基因 身材矮小
RGS2抑制大鼠脑出血后炎症反应的实验研究
8
作者 甘荟 陈辉 +4 位作者 张莉 肖涵 王露 蒋宁 梁平 《重庆医科大学学报》 CAS CSCD 北大核心 2019年第5期588-591,共4页
目的:探讨 G 蛋白信号调节因子 2(regulators of G-protein signalling,RGS2)抑制大鼠脑出血(intracerebral hemorrhage,ICH)继发的炎症反应。方法:69 只大鼠分为假手术组、ICH 组、阴性对照慢病毒+ICH 组、RGS2 过表达慢病毒+ICH 组和... 目的:探讨 G 蛋白信号调节因子 2(regulators of G-protein signalling,RGS2)抑制大鼠脑出血(intracerebral hemorrhage,ICH)继发的炎症反应。方法:69 只大鼠分为假手术组、ICH 组、阴性对照慢病毒+ICH 组、RGS2 过表达慢病毒+ICH 组和时间窗组,前 4 组各组 12 只 SD 大鼠,时间窗组下设 normal、ICH6h、ICH12h、ICH24h、ICH48h、ICH72h 和 ICH7d,每小组各 3 只 SD 大鼠。基底节注射 VⅡ型胶原酶,建立大鼠 ICH 模型,对大鼠 ICH 后 3 d 进行神经功能评分,干湿法检测脑含水量,采用 Westernblot 和 ELISA 法测定血肿周围脑组织中肿瘤坏死因子-α(tumor necrosis factor,TNF-α)和白介素-1β(interleukin-1,IL-1β)的含量,免疫荧光染色方法检测病灶周边髓过氧化物酶(myeloperoxidase,MPO)表达情况。结果:①RGS2 在 ICH 后表达量从(0.5792±0.019 8)开始升高,并且在术后 12 h 达到峰值(0.809 3±0.076 2),然后降低,术后 7 d 恢复正常(0.600 0±0.083 0)。②与ICH组脑含水量(0.804 7±0.003 4)和神经功能评分(11.570 0±2.070 0)相比,RGS2 过表达慢病毒+ICH 组在 ICH 术后 3 d 脑水肿明显减轻(0.794 0±0.001 5),神经功能评分明显改善(7.429 0±0.976 0)。③RGS2 过表达慢病毒+ICH 组术后 3 d 血肿周边 TNF-α和 IL-1β的表达(1.407±0.055、0.762±0.046)明显低于 ICH 组(1.743±0.179、0.917±0.085),RGS2 过表达慢病毒+ICH 组 TNF-α和 IL-1β的 A 值(32.92±1.13、32.84±1.08)低于 ICH 组 A 值(40.38±0.99、39.29±1.39)。④RGS2 过表达慢病毒+ICH 组大鼠病灶周边 MPO 的表达明显低于 ICH 组和阴性对照慢病毒+ICH 组。结论:RGS2 抑制大鼠脑出血后血肿周边区TNF-α、IL-1β和 MPO 表达,能减轻脑水肿,改善神经功能,抑制脑出血后的炎症反应。 展开更多
关键词 脑出血 G 蛋白信号调节因子 2 炎症反应
Clinical characteristics of non-alcoholic fatty liver disease in Chinese adult hypopituitary patients 预览
9
作者 Xian-Xian Yuan Hui-Juan Zhu +7 位作者 Hui Pan Shi Chen Ze-Yu Liu Yue Li Lin-Jie Wang Lin Lu Hong-Bo Yang Feng-Ying Gong 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第14期1741-1752,共12页
BACKGROUND Patients with hypothalamic-pituitary disease have the feature of central obesity, insulin resistance, and dyslipidemia, and there is increased prevalence of liver dysfunction consistent with non-alcoholic f... BACKGROUND Patients with hypothalamic-pituitary disease have the feature of central obesity, insulin resistance, and dyslipidemia, and there is increased prevalence of liver dysfunction consistent with non-alcoholic fatty liver disease (NAFLD) in this population. The causes of hypopituitarism in the reported studies varied and combined pituitary hormone deficiency including central diabetes insipidus is much common in this population. This retrospective cross-sectional study was performed to analyze the clinical characteristics and related factors with NAFLD and cirrhosis in Chinese adult hypopituitary/panhypopituitary patients. AIM To analyze the clinical characteristics of and related risk factors for NAFLD in Chinese adult hypopituitary patients. METHODS Adult Chinese patients with hypopituitarism and/or panhypopituitarism were enrolled at the Pituitary Center of Peking Union Medical College Hospital between August 2012 and April 2018. According to abdominal ultrasonography, these patients were divided into an NAFLD (-) group and an NAFLD (+) group, and the latter was further divided into an NAFLD group and a cirrhotic group. The data, such as patient characteristics, diagnosis, and treatment, were extracted from medical records, and statistical analysis was performed. RESULTS A total of 36 male and 14 female adult Chinese patients with hypopituitarism were included in this retrospective study;43 (87.0%) of these patients exhibited growth hormone (GH) deficiency, and 39 (78.3%) had diabetes insipidus. A total of 27 (54.0%) patients were diagnosed with NAFLD, while seven patients were cirrhotic. No significant differences were noted in serum GH or insulin-like growth factor 1 among patients with cirrhosis, subjects with NAFLD, and those without NAFLD. However, plasma osmolality and serum sodium concentration of the cirrhotic patients were 314.9 mOsm/kgH2O and 151.0 mmol/L, respectively, which were significantly higher than those of the NAFLD patients (P = 0.036 and 0.042, respectively). Overweight/obesity and i 展开更多
关键词 HYPOPITUITARISM Non-alcoholic FATTY liver disease CIRRHOSIS Diabetes insipidus Plasma OSMOLALITY
在线阅读 免费下载
Effects of Niaoduqing Particles(尿毒清颗粒)on Delaying Progression of Renal Dysfunction:A Post-trial,Open-Label,Follow-up Study
10
作者 ZHENG Ying WANG Nian-song +24 位作者 LIU Yu-ning HE Li-qun JIAN Gui-hua LIU Xu-sheng NI Zhao-hui CHENG Xiao-hong LIN Hong-li ZHOU Wen-hua WANG Ya-ping FANG Jing-ai HE Ya-ni YANG Hong-tao ZHAO Li-juan DING Hart-lu WANG Li-hua YU Ren-huan LI Wen-ge YE Zhi-ming GUO Wang ZHAN Yong-li MAO Hui-juan HU Zhao YAO Chen CAI Guang-yan CHEN Xiang-mei 《中国结合医学杂志:英文版》 SCIE CAS CSCD 2019年第3期168-174,共7页
Objective:To follow up the participants of the randomized clinical trial "Efficacy and Safety of Niaoduqing Particles(尿毒清颗粒) for Delaying Moderate-to-Severe Renal Dysfunction", and assess the long-term ... Objective:To follow up the participants of the randomized clinical trial "Efficacy and Safety of Niaoduqing Particles(尿毒清颗粒) for Delaying Moderate-to-Severe Renal Dysfunction", and assess the long-term effects of Niaoduqing Particles on delaying the progression of renal dysfunction. Methods: Participants, who had previously been randomly assigned to receive Niaoduqing Particles or placebo for 24 weeks(146 cases in each group), were invited to follow-up and all were administered Niaoduqing Particles 5 g thrice daily and 10 g before bedtime for 24 weeks. The primary endpoints were changes in baseline serum creatinine(Scr) and estimated glomerular filtration rate(e GFR) after completion of the open-label treatment period. Results: After the double-blind period, the median(interquartile range) changes in Scr were 1.1(–13.0–24.1) and 11.7(–2.6–42.9) μmol/L for the Niaoduqing Particle and placebo groups, respectively(P=0.008), and the median changes in e GFRs were –0.2(–4.3–2.7) and –2.21(–5.7–0.8) mL·min^-1·1.73 m^-2, respectively(P=0.016). There were significant differences in the double-blind period changes in renal function between groups. After the open-label period, the median changes in Scr were 9.0(–10.0–41.9) and 17.5(–6.0–50.0) μmol/L for the Niaoduqing Particle and placebo groups according to baseline grouping, respectively(P=0.214), and the median changes in eGFRs were –2.3(–6.4–1.9) and –3.7(–7.5–1.1) mL·min^-1·1.73 m^-2, respectively(P=0.134). There were no statistical differences in the open-label period changes in renal function between groups. The eGFR reduction of participants who accepted Niaoduqing Particle treatment for 48 weeks was projected to 2.5 m L·min^-1·1.73 m^-2 per year. Conclusions: Niaoduqing Particles appear to have long-term efficacy for patients with moderate-to-severe renal dysfunction. Although there was no statistical difference, the early use of Niaoduqing Paticles seems to ameliorate the worsening of renal function. 展开更多
关键词 chronic kidney disease moderate-to-severe renal dysfunction NIAODUQING PARTICLES post-trial follow-up Chinese medicine
霍山石斛糖蛋白的提取方法优选及抗氧化活性分析 预览
11
作者 邓辉 崔绍进 +3 位作者 朱富成 韩邦兴 陈乃东 陈乃富 《生物学杂志》 CAS CSCD 北大核心 2019年第2期107-111,共5页
以道地药材——霍山石斛鲜茎为试验材料,分别按提取蛋白的盐溶液浸法和提取多糖的水提醇沉法,在4℃条件下提取糖蛋白,考察比对其提取效果,以及不同糖蛋白组分的抗氧化活性。由两种方法获得的提取液分别经硫酸铵分级沉淀和乙醇分级沉淀,... 以道地药材——霍山石斛鲜茎为试验材料,分别按提取蛋白的盐溶液浸法和提取多糖的水提醇沉法,在4℃条件下提取糖蛋白,考察比对其提取效果,以及不同糖蛋白组分的抗氧化活性。由两种方法获得的提取液分别经硫酸铵分级沉淀和乙醇分级沉淀,获得6组糖蛋白提取液,经SDS-PAGE电泳,蛋白染色和糖染色确定提取效果。结果表明:盐溶液浸提法获得的糖蛋白谱带在浓度、数量和分子量范围上都显著高于水提醇沉法。体外抗氧化试验表明,谱带多态性较高的糖蛋白提取液具有较高的清除超氧阴离子自由基和羟基自由基的能力。实验进一步通过单因素和正交试验确定了盐溶液浸提霍山石斛糖蛋白的最佳条件:NaCl溶液浓度0.3mol/L、液料比16∶1、提取时间12h。在此条件下,粗糖蛋白得率(12.56±0.35)%。 展开更多
关键词 霍山石斛 糖蛋白 提取方法 抗氧化活性
在线阅读 下载PDF
Application of next-generation sequencing technology to precision medicine in cancer: joint consensus of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology 预览
12
作者 Xuchao Zhang Zhiyong Liang +47 位作者 Shengyue Wang Shun Lu Yong Song Ying Cheng Jianming Ying Weiping Liu Yingyong Hou Yangqiu Li Yi Liu Jun Hou Xiufeng Liu Jianyong Shao Yanhong Tai Zheng Wang Li Fu Hui Li Xiaojun Zhou Hua Bai Mengzhao Wang You Lu Jinji Yang Wenzhao Zhong Qing Zhou Xuening Yang Jie Wang Cheng Huang Xiaoqing Liu Xiaoyan Zhou Shirong Zhang Hongxia Tian Yu Chen Ruibao Ren Ning Liao Chunyan Wu Zhongzheng Zhu Hongming Pan Yanhong Gu Liwei Wang Yunpeng Liu Suzhan Zhang Tianshu Liu Gong Chen Zhimin Shao Binghe Xu Qingyuan Zhang Ruihua Xu Lin Shen Yilong Wu 《癌症生物学与医学:英文版》 CAS CSCD 2019年第1期189-204,共16页
Next-generation sequencing(NGS) technology is capable of sequencing millions or billions of DNA molecules simultaneously.Therefore, it represents a promising tool for the analysis of molecular targets for the initial ... Next-generation sequencing(NGS) technology is capable of sequencing millions or billions of DNA molecules simultaneously.Therefore, it represents a promising tool for the analysis of molecular targets for the initial diagnosis of disease, monitoring of disease progression, and identifying the mechanism of drug resistance. On behalf of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology(CSCO) and the China Actionable Genome Consortium(CAGC), the present expert group hereby proposes advisory guidelines on clinical applications of NGS technology for the analysis of cancer driver genes for precision cancer therapy. This group comprises an assembly of laboratory cancer geneticists, clinical oncologists, bioinformaticians,pathologists, and other professionals. After multiple rounds of discussions and revisions, the expert group has reached a preliminary consensus on the need of NGS in clinical diagnosis, its regulation, and compliance standards in clinical sample collection. Moreover, it has prepared NGS criteria, the sequencing standard operation procedure(SOP), data analysis, report, and NGS platform certification and validation. 展开更多
关键词 Next-generation SEQUENCING TECHNOLOGY CANCER consensus
在线阅读 下载PDF
Low-Frequency Electroacupuncture Alleviates Chronic Constrictive Injury-Induced Mechanical Allodynia by Inhibiting NR2B Upregulation in Ipsilateral Spinal Dorsal Horn in Rats
13
作者 ZHAO Wen-sheng JIANG Zhen-ni +3 位作者 SHI Hui XU Lu-Lu YANG Yue WANG Ying-chao 《中国结合医学杂志:英文版》 SCIE CAS CSCD 2019年第6期462-467,共6页
Objective: To study the effects of electroacupuncture(EA) in chronic constrictive injury(CCI) rat model and the expression of N-methyl-D-aspartate receptor type 2B(NR2B) in ipsilateral spinal dorsal horn in rats to ex... Objective: To study the effects of electroacupuncture(EA) in chronic constrictive injury(CCI) rat model and the expression of N-methyl-D-aspartate receptor type 2B(NR2B) in ipsilateral spinal dorsal horn in rats to explore the analgesic mechanisms of EA. Methods: According to the random number table, totally 180 rats were evenly divided into a sham group, a CCI group, and an EA group. CCI model was conducted with four4–0 chromic gut ligatures loosely ligated around the left sciatic nerve 1 cm above the trifurcation. Rats in the EA group received 2 Hz EA therapy bilaterally at acupoints of Zusanli(ST 36) and Sanyinjiao(SP 6) once daily(30 min/d) for 30 days after surgery. Paw withdrawal thresholds(PWTs) were measured on 0(baseline), 1, 3, 7, 15,30 days after surgery. Rats were sacri?ced on 0, 1, 3, 7, 15 and 30 days after surgery, and the L4–5 segments of spinal cord were removed to detect the expression of NR2B by immunohistochemistry and quantitative polymerase chain reaction. Results: PWTs in the CCI group were signi?cantly lower than the sham group at Day1–30 after surgery, and reached its lowest at Day 1(P<0.01). After EA treatment, the PWTs recovered rapidly and were signi?cantly higher than those in the CCI group on 3, 7, 15 and 30 days after surgery(P<0.01). The numbers of NR2B-immunoreactive cells of the CCI group signi?cantly increased after CCI surgery compared with the sham group(P<0.01). Compared with the CCI group, stimulation of EA markedly decreased the numbers of NR2B-immunoreactive cells at Day 3, 7, 15 and 30(P<0.05). In the sham group, NR2B mRNA was expressed at a low level. It increased after CCI surgery, which increased rapidly at Day 7(P<0.01) and reached its peak value at Day 15(P<0.01). After EA stimulation, relative quantity of NR2B mRNA expression was less than that in the CCI group at Day 15 and 30(P<0.05). Conclusions: Low frequency of EA had antinociceptive effect in CCI rat model. The analgesic effects of EA might be through the inhibition of NR2B. 展开更多
关键词 SCIATICA ELECTROACUPUNCTURE CHRONIC CONSTRICTIVE INJURY spinal dorsal horn N?methyl-D-aspartate receptor type 2B Chinese medicine
亚急性乌头碱中毒对小鼠血液生理指标的影响
14
作者 刘彦兵 王辉 +6 位作者 路浩 杨琳 杨涵琪 郭蓉 姚茹 穆璐 赵宝玉 《中国兽医学报》 CAS CSCD 北大核心 2019年第2期307-312,共6页
为了探讨乌头碱对小鼠血液生理指标的影响,将120只小鼠随机分成4组,即对照组(生理盐水)、低剂量组(0.14μmol/L)、中剂量组(0.28μmol/L)、高剂量组(0.56μmol/L),连续灌胃30d。分别在10,20,30d每次每组随机采集10只小鼠血液,用全自动... 为了探讨乌头碱对小鼠血液生理指标的影响,将120只小鼠随机分成4组,即对照组(生理盐水)、低剂量组(0.14μmol/L)、中剂量组(0.28μmol/L)、高剂量组(0.56μmol/L),连续灌胃30d。分别在10,20,30d每次每组随机采集10只小鼠血液,用全自动血液细胞分析仪测定血液中白细胞(WBC)、淋巴细胞(LymPh)、单核细胞(Mon)、中心粒细胞(Gran)、红细胞(RBC)、血红蛋白(HGB)、红细胞压积(HCT)和血小板(PLT)的含量。结果显示,高剂量组小鼠肝脏、肺脏、大脑的脏器系数明显增加(P<0.05或P<0.01)。随毒物浓度的增加,各染毒组WBC、LymPh、Mon、Gran先升高后降低,与对照组相比,高剂量组变化最为明显,且差异显著(P<0.05或P<0.01)。RBC、HGB、HCT和PLT持续降低,在20和30d时,除低剂量组外,差异显著(P<0.05或P<0.01)。结果表明,不同浓度乌头碱对小鼠血液生理指标有显著影响,且存在明显时间和剂量效应关系,可引起动物贫血,降低机体免疫机能。 展开更多
关键词 乌头碱 亚急性中毒 小鼠 脏器系数 血液生理指标
DCZ0801,a novel compound,induces cell apoptosis and cell cycle arrest via MAPK pathway in multiple myeloma
15
作者 Ting Zhang Bo Li +15 位作者 Qilin Feng Zhijian Xu Cheng Huang Huiqun Wu Zhangbo Chen Liangning Hu Lu Gao Peng Liu Guang Yang Hui Zhang Kang Lu Tingye Li Yi Tao Xiaosong Wu Jumei Shi Weiliang Zhu 《生物化学与生物物理学报:英文版》 SCIE CAS CSCD 2019年第5期517-523,共7页
Multiple myeloma(MM)is a refractory malignant hematological malignancy,and many therapeutic strategies have been developed to cure patients with MM.DCZ0801 is a compound that consists of oxophenamide and pterostilbene... Multiple myeloma(MM)is a refractory malignant hematological malignancy,and many therapeutic strategies have been developed to cure patients with MM.DCZ0801 is a compound that consists of oxophenamide and pterostilbene.The role of these compounds in hematological cancers such as MM has yet to be studied.In this study,we explored the potential mechanism of DCZ0801 action,its anti-tumor activity both in vitro and in vivo on MM.This study was carried out via cell cycle proliferation assay,apoptotic analysis,western blot analysis,and examination of xenotransplantation model of tumors.The in vitro studies revealed that DCZ0801 could inhibit cell proliferation and induce apoptosis by regulating both caspase-dependent and mitogen-activated protein kinase signaling pathways,inducing S-phase arrest of the cell cycle related to downregulation of CDK2,cyclin-A2,and CDC25A protein expression.The in vivo studies showed that DCZ0801 could significantly reduce the size of the tumors in nude mice.Our results demonstrated that DCZ0801 may emerge as the new therapeutic option for the patient with MM. 展开更多
关键词 DCZ0801 multiple MYELOMA apoptosis cell CYCLE MAPK
异型雄性罗氏沼虾遗传多样性的微卫星分析 预览
16
作者 吕敏 黄光华 +8 位作者 李旻 杨琼 卢小花 甘晖 阮志德 黄立斌 杨彦豪 卢天和 马华威 《水产科学》 CAS CSCD 北大核心 2019年第3期355-360,共6页
利用微卫星DNA标记研究3种近亲交配形态型雄性罗氏沼虾遗传多样性,所用虾样均采自国家级(南宁)罗氏沼虾良种场,共150尾成年近亲交配的雄虾,其中蓝色长臂型、橙色长臂型、小个体型各50尾。研究结果表明,3组样品的生长发育差异显著,蓝色... 利用微卫星DNA标记研究3种近亲交配形态型雄性罗氏沼虾遗传多样性,所用虾样均采自国家级(南宁)罗氏沼虾良种场,共150尾成年近亲交配的雄虾,其中蓝色长臂型、橙色长臂型、小个体型各50尾。研究结果表明,3组样品的生长发育差异显著,蓝色长臂型、橙色长臂型、小个体型各组中微卫星位点的总等位基因分别为24、28 和22,3组样品所有位点的平均观察杂合度为0.474~0.556。其平均群体内近交系数为-0.0189,说明杂合子过剩,对比分析和群体间分化系数表明,3组雄虾遗传差异明显,最大遗传间距位于蓝色长臂型和小个体型,最小的遗传间距位于蓝色长臂型和橙色长臂型。研究结果将为罗氏沼虾优质父本选育和调控雄性遗传发育提供基础性遗传信息。 展开更多
关键词 微卫星分子标记 罗氏沼虾 形态型 基因多样性
在线阅读 下载PDF
经血浆置换治疗乙肝相关慢加急性肝衰竭患者短期预后模型的研究 预览
17
作者 甘琼萍 谭可平 +3 位作者 陈婵 陆晖 陆鹏 陈茂伟 《中国现代医生》 2019年第5期27-30,F0003共5页
目的建立经血浆置换(PE)治疗乙肝相关慢加急性肝衰竭(HBV-ACLF)患者预后判断模型。方法回顾性分析广西医科大学第一附属医院2012年1月~2017年10月收治的138例经PE治疗的HBV-ACLF患者资料,根据PE治疗后12周存活情况分为生存组与死亡组,采... 目的建立经血浆置换(PE)治疗乙肝相关慢加急性肝衰竭(HBV-ACLF)患者预后判断模型。方法回顾性分析广西医科大学第一附属医院2012年1月~2017年10月收治的138例经PE治疗的HBV-ACLF患者资料,根据PE治疗后12周存活情况分为生存组与死亡组,采用Logistic回归分析可能影响预后的因素并建立回归模型,使用ROC曲线下面积(AUC)评价模型的预测价值。结果生存组56例,死亡组82例。单因素分析显示年龄、总胆红素(TBIL)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、甘油三酯(TG)、肝性脑病(HE)、肝肾综合征(HRS)、MELD评分、Child-Turcotte-Pugh(CTP)评分9个因素在两组间差异有统计学意义(P<0.05)。经Logistic回归分析筛选出年龄、TBIL、AST、肝性脑病、肝肾综合征5个因素用于回归模型的拟合。模型的ROC曲线下面积为0.879(95%CI:0.822-0.936),灵敏度为75.61%,特异度为85.71%。模型与CTP评分、MELD评分的AUC对比差异无统计学意义(P>0.05)。结论研究中拟合的Logistic回归模型对预后评估有一定参考价值,与CTP评分、MELD评分有同等预后判断价值。 展开更多
关键词 血浆置换 慢性乙型肝炎 慢加急性肝衰竭 预后模型
在线阅读 下载PDF
低峰均比OFDM雷达信号的收发系统设计
18
作者 赵晖 陆珺 陆健鲲 《现代雷达》 CSCD 北大核心 2019年第1期73-79,共7页
射频性能和计算量的平衡是正交频分复用(OFDM)雷达信号得以应用的前提。文中使用一种子载波频谱幅度滚降的OFDM雷达信号加权系数生成方法,通过调整滚降系数来控制过渡带中的子载波功率,保证低功率峰均比的前提下减少直接数字式频率合成(... 射频性能和计算量的平衡是正交频分复用(OFDM)雷达信号得以应用的前提。文中使用一种子载波频谱幅度滚降的OFDM雷达信号加权系数生成方法,通过调整滚降系数来控制过渡带中的子载波功率,保证低功率峰均比的前提下减少直接数字式频率合成(DDS)中有限长单位冲激响应(FIR)滤波器所需阶数。通过设计低计算复杂度的产生与接收方案,分析了饱和放大器幅度非线性以及模拟滤波器相位非线性对OFDM雷达信号带来的影响,使其在现有雷达硬件平台上可实现。仿真和测试验证了文中提出方法的有效性。 展开更多
关键词 正交频分复用雷达信号 功率峰均比 相位调制 饱和放大 相位非线性 软件无线电
血清HER2-ECD水平在乳腺癌新辅助化疗中的应用价值分析 预览
19
作者 孙佳俊 卢仁泉 +2 位作者 郑慧 陆立 郭林 《中国癌症杂志》 CAS CSCD 北大核心 2019年第1期32-36,共5页
背景与目的:人类表皮生长因子受体-2(human epidermal growth factor receptor-2,HER2)是一种原癌基因所表达的蛋白,HER2阳性往往预示着肿瘤进展快、容易发生淋巴结或血道转移,对新辅助化疗不敏感,预后不佳。本研究通过对乳腺癌患者血清... 背景与目的:人类表皮生长因子受体-2(human epidermal growth factor receptor-2,HER2)是一种原癌基因所表达的蛋白,HER2阳性往往预示着肿瘤进展快、容易发生淋巴结或血道转移,对新辅助化疗不敏感,预后不佳。本研究通过对乳腺癌患者血清HER2胞外段(extracellular domain,ECD)水平与患者临床新辅助化疗的反应性的比较,来评估血清HER2-ECD在新辅助化疗评估中的价值。方法:收集507例乳腺癌患者的治疗前血清样本,利用χ2检验比较血清HER2-ECD与患者年龄、分期、组织雌激素受体、孕激素受体、Ki-67及HER2的关系,对其中48例HER2表达阳性的行新辅助化疗的乳腺癌患者予以随访,单因素分析治疗前后血清HER2-ECD水平变化与新辅助化疗疗效之间的关系。结果:通过对507例患者血清HER2-ECD和临床病理特征的比较发现,在年龄 >50岁、Ⅲ~Ⅳ期、组织雌激素受体(-)、孕激素受体(-)、Ki-67 >20%及HER2(+)的患者中血清HER2-ECD阳性率较高。对48例组织HER2阳性的患者随访发现,血清HER2-ECD在治疗2个周期后即出现大幅下降,从18.10 ng/mL(13.20~28.95 ng/mL)降低到11.20 ng/mL(9.80~12.75 ng/mL)(P<0.01)。对48例患者进行疗效评估发现,新辅助化疗2个周期后血清HER2-ECD阴性的患者其客观缓解率(94.7%,36/38)显著高于治疗后血清HER2-ECD阳性的患者(60.0%,6/10)(P<0.05)。结论:乳腺癌患者血清HER2-ECD升高与其年龄、分期、组织雌激素受体、孕激素受体及Ki-67相关,且新辅助化疗2个周期后的血清HER2-ECD水平对新辅助化疗的疗效有一定的评估作用。 展开更多
关键词 乳腺癌 新辅助化疗 疗效评估
在线阅读 下载PDF
乌鲁木齐县0~6岁儿童家长免疫接种知识知晓率及影响因素分析 预览
20
作者 王辉 陆璐 +1 位作者 马艳 宋秋楠 《新疆医学》 2019年第3期265-268,共4页
目的了解乌鲁木齐县儿童家长免疫接种知识知晓率及影响因素,为开展免疫接种工作提供参考。方法采用自编乌鲁木齐县(0~6)岁儿童家长免疫接种知识知晓情况调查问卷,分层随机抽取乌鲁木齐县(0~6)周岁儿童的家长进行问卷调查。结果乌鲁木齐... 目的了解乌鲁木齐县儿童家长免疫接种知识知晓率及影响因素,为开展免疫接种工作提供参考。方法采用自编乌鲁木齐县(0~6)岁儿童家长免疫接种知识知晓情况调查问卷,分层随机抽取乌鲁木齐县(0~6)周岁儿童的家长进行问卷调查。结果乌鲁木齐县儿童家长免疫接种知识得分M(P25,P75)为15(10,17);影响因素的单因素卡方检验及多因素Logistic回归分析结果显示儿童家长免疫接种知识知晓率的主要影响因素为与儿童的关系、受教育水平、职业,家庭年经济收入(P均<0.05);儿童家长免疫接种知识主要来源为接种门诊医生告知(85.06%)、阅读宣传材料(54.36%);97.93%的家长赞同免疫接种,90.87%的家长能按接种门诊医生告知的时间带孩子进行免疫接种。结论乌鲁木齐县(0~6)岁儿童家长免疫接种知识知晓情况一般,应根据文化程度、职业等,加强免疫接种知识宣传,提高家长认知,促进规划免疫工作的顺利开展。 展开更多
关键词 家长 预防接种 知晓率
在线阅读 下载PDF
上一页 1 2 250 下一页 到第
使用帮助 返回顶部 意见反馈